atcc baa 3252  (ATCC)

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Reduction in planktonic cells growth of Acinetobacter baumannii reference strains ATCC 19606 and ATCC BAA-3252 treated with different concentrations of the Arnica montana L. ethanolic extract after 24 h of incubation at a temperature of 37 °C in aerobic conditions. Explanatory notes: mean OD 600 ± SD values for both strains control without extract (100% growth of the non-treated bacterial strains) were 1.0206 ± 0.019 and 1.0295 ± 0.028 for A. baumannii ATCC 19606 and A. baumannii ATCC BAA-3252. Statistical data were analyzed using the Mann–Whitney U nonparametric test (with continuity correction). The results marked with an asterisk (*) are statistically significant at p < 0.05; (**) are significant at p < 0.01. Data were based on ten replicates for each bacterial strain.

Article Snippet: The OD 600 values for A. baumannii ATCC 19606 ranged from 1.032 ± 0.040 to 0.962 ± 0.063, and for A. baumannii ATCC BAA-3252 ranged from 1.019 ± 0.016 to 0.943 ± 0.038 ( ).

Techniques: Incubation, Control, MANN-WHITNEY

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Light inverted microscopy observation of the subinhibitory MIC antibiofilm effects of the Arnica montana ethanolic extract on Acinetobacter baumannii reference strains (ATCC 19606 and ATCC BAA-3252 under stationary conditions (2000× magnification, 20 µm scale bar, Olympus DP22 automated inverted light microscope, CellSens Dimensions 2.3 software, Olympus Corporation, Tokio, Japan). Explanatory notes: OD 570 ± SD—median values of OD 570 of four replicates with standard deviation; MAOB—measured area occupied by biofilm (%) calculated via ImageJ 1.54g software (US National Institutes of Health, Bethesda, Maryland, USA); strain control without extract means the 100% growth of the non-treated bacterial strain.

Article Snippet: The OD 600 values for A. baumannii ATCC 19606 ranged from 1.032 ± 0.040 to 0.962 ± 0.063, and for A. baumannii ATCC BAA-3252 ranged from 1.019 ± 0.016 to 0.943 ± 0.038 ( ).

Techniques: Inverted Microscopy, Light Microscopy, Software, Standard Deviation, Control

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: The inhibition of biofilm-forming cell growth depends on the Acinetobacter baumannii strain and the extract and/or alcohol used. Explanatory notes: biofilm inhibition rated on a scale of 0–100%; values < 0% indicated bacterial biofilm growth enhancement; values of 0–50% indicated weak anti-biofilm activity; values > 50% indicated good biofilm inhibition; Statistical data were analyzed using the Mann–Whitney U test between bacterial strains treated with plant extract or between bacterial strains treated with ethanol. The asterisks mean statistically significant differences as follows: (*) are statistically significant at p < 0.05; (**) are significant at p < 0.01. Data were based on four replicates.

Article Snippet: The OD 600 values for A. baumannii ATCC 19606 ranged from 1.032 ± 0.040 to 0.962 ± 0.063, and for A. baumannii ATCC BAA-3252 ranged from 1.019 ± 0.016 to 0.943 ± 0.038 ( ).

Techniques: Inhibition, Activity Assay, MANN-WHITNEY, Plant Extract

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Relative expression of target genes in Acinetobacter baumannii ATCC 19606 subjected to ½ MIC (½ MIC of alcoholic A. montana flower extract); ¼ MIC (¼ MIC of alcoholic A. montana flower extract), EtOH ½ MIC (½ MIC of ethanol), EtOH ¼ MIC (¼ MIC of ethanol). The data represent the means ± SDs ( n = 3). Means concerning the same gene, followed by different letters, are significantly different according to Tukey’s test at p < 0.05.

Article Snippet: The OD 600 values for A. baumannii ATCC 19606 ranged from 1.032 ± 0.040 to 0.962 ± 0.063, and for A. baumannii ATCC BAA-3252 ranged from 1.019 ± 0.016 to 0.943 ± 0.038 ( ).

Techniques: Expressing

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Melting-curve analysis of all selected biofilm-related genes in Acinetobacter baumannii ATCC 19606.

Article Snippet: The OD 600 values for A. baumannii ATCC 19606 ranged from 1.032 ± 0.040 to 0.962 ± 0.063, and for A. baumannii ATCC BAA-3252 ranged from 1.019 ± 0.016 to 0.943 ± 0.038 ( ).

Techniques:

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Relative expression of target genes in Acinetobacter baumannii ATCC BAA-3252 subjected to ½ MIC (½ MIC of alcoholic A. montana flower extract), ¼ MIC (¼ MIC of alcoholic A. montana flower extract), EtOH ½ MIC (½ MIC of ethanol), EtOH ¼ MIC (¼ MIC of ethanol). The data represent the means ± SDs ( n = 3). Means concerning the same gene, followed by different letters, are significantly different according to Tukey’s test at p < 0.05.

Article Snippet: The OD 600 values for A. baumannii ATCC 19606 ranged from 1.032 ± 0.040 to 0.962 ± 0.063, and for A. baumannii ATCC BAA-3252 ranged from 1.019 ± 0.016 to 0.943 ± 0.038 ( ).

Techniques: Expressing

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Reduction in planktonic cells growth of Acinetobacter baumannii reference strains ATCC 19606 and ATCC BAA-3252 treated with different concentrations of the Arnica montana L. ethanolic extract after 24 h of incubation at a temperature of 37 °C in aerobic conditions. Explanatory notes: mean OD 600 ± SD values for both strains control without extract (100% growth of the non-treated bacterial strains) were 1.0206 ± 0.019 and 1.0295 ± 0.028 for A. baumannii ATCC 19606 and A. baumannii ATCC BAA-3252. Statistical data were analyzed using the Mann–Whitney U nonparametric test (with continuity correction). The results marked with an asterisk (*) are statistically significant at p < 0.05; (**) are significant at p < 0.01. Data were based on ten replicates for each bacterial strain.

Article Snippet: Moderate inhibition of the growth of planktonic cells of these bacteria was caused by the presence of >468.8 μg/mL of the extract, with growth inhibition ranging from 87.4 ± 0.0046% to 96.6 ± 0.0136% for A. baumannii ATCC 19606 and from 95.4 ± 0.0005% to 96.9 ± 0.0042% for A. baumannii ATCC BAA-3252 (statistically significant difference was noted at every extract concentrations except 30,000 μg/mL; p < 0.0001).

Techniques: Incubation, Control, MANN-WHITNEY

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Light inverted microscopy observation of the subinhibitory MIC antibiofilm effects of the Arnica montana ethanolic extract on Acinetobacter baumannii reference strains (ATCC 19606 and ATCC BAA-3252 under stationary conditions (2000× magnification, 20 µm scale bar, Olympus DP22 automated inverted light microscope, CellSens Dimensions 2.3 software, Olympus Corporation, Tokio, Japan). Explanatory notes: OD 570 ± SD—median values of OD 570 of four replicates with standard deviation; MAOB—measured area occupied by biofilm (%) calculated via ImageJ 1.54g software (US National Institutes of Health, Bethesda, Maryland, USA); strain control without extract means the 100% growth of the non-treated bacterial strain.

Article Snippet: Moderate inhibition of the growth of planktonic cells of these bacteria was caused by the presence of >468.8 μg/mL of the extract, with growth inhibition ranging from 87.4 ± 0.0046% to 96.6 ± 0.0136% for A. baumannii ATCC 19606 and from 95.4 ± 0.0005% to 96.9 ± 0.0042% for A. baumannii ATCC BAA-3252 (statistically significant difference was noted at every extract concentrations except 30,000 μg/mL; p < 0.0001).

Techniques: Inverted Microscopy, Light Microscopy, Software, Standard Deviation, Control

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: The inhibition of biofilm-forming cell growth depends on the Acinetobacter baumannii strain and the extract and/or alcohol used. Explanatory notes: biofilm inhibition rated on a scale of 0–100%; values < 0% indicated bacterial biofilm growth enhancement; values of 0–50% indicated weak anti-biofilm activity; values > 50% indicated good biofilm inhibition; Statistical data were analyzed using the Mann–Whitney U test between bacterial strains treated with plant extract or between bacterial strains treated with ethanol. The asterisks mean statistically significant differences as follows: (*) are statistically significant at p < 0.05; (**) are significant at p < 0.01. Data were based on four replicates.

Article Snippet: Moderate inhibition of the growth of planktonic cells of these bacteria was caused by the presence of >468.8 μg/mL of the extract, with growth inhibition ranging from 87.4 ± 0.0046% to 96.6 ± 0.0136% for A. baumannii ATCC 19606 and from 95.4 ± 0.0005% to 96.9 ± 0.0042% for A. baumannii ATCC BAA-3252 (statistically significant difference was noted at every extract concentrations except 30,000 μg/mL; p < 0.0001).

Techniques: Inhibition, Activity Assay, MANN-WHITNEY, Plant Extract

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Relative expression of target genes in Acinetobacter baumannii ATCC 19606 subjected to ½ MIC (½ MIC of alcoholic A. montana flower extract); ¼ MIC (¼ MIC of alcoholic A. montana flower extract), EtOH ½ MIC (½ MIC of ethanol), EtOH ¼ MIC (¼ MIC of ethanol). The data represent the means ± SDs ( n = 3). Means concerning the same gene, followed by different letters, are significantly different according to Tukey’s test at p < 0.05.

Article Snippet: Moderate inhibition of the growth of planktonic cells of these bacteria was caused by the presence of >468.8 μg/mL of the extract, with growth inhibition ranging from 87.4 ± 0.0046% to 96.6 ± 0.0136% for A. baumannii ATCC 19606 and from 95.4 ± 0.0005% to 96.9 ± 0.0042% for A. baumannii ATCC BAA-3252 (statistically significant difference was noted at every extract concentrations except 30,000 μg/mL; p < 0.0001).

Techniques: Expressing

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Melting-curve analysis of all selected biofilm-related genes in Acinetobacter baumannii ATCC 19606.

Article Snippet: Moderate inhibition of the growth of planktonic cells of these bacteria was caused by the presence of >468.8 μg/mL of the extract, with growth inhibition ranging from 87.4 ± 0.0046% to 96.6 ± 0.0136% for A. baumannii ATCC 19606 and from 95.4 ± 0.0005% to 96.9 ± 0.0042% for A. baumannii ATCC BAA-3252 (statistically significant difference was noted at every extract concentrations except 30,000 μg/mL; p < 0.0001).

Techniques:

Journal: Genes

Article Title: Preliminary Assessment of Arnica montana L. Extract: Antimicrobial Activity Against Acinetobacter baumannii and Biofilm-Related Gene Expression Profiling

doi: 10.3390/genes16121473

Figure Lengend Snippet: Relative expression of target genes in Acinetobacter baumannii ATCC BAA-3252 subjected to ½ MIC (½ MIC of alcoholic A. montana flower extract), ¼ MIC (¼ MIC of alcoholic A. montana flower extract), EtOH ½ MIC (½ MIC of ethanol), EtOH ¼ MIC (¼ MIC of ethanol). The data represent the means ± SDs ( n = 3). Means concerning the same gene, followed by different letters, are significantly different according to Tukey’s test at p < 0.05.

Article Snippet: Moderate inhibition of the growth of planktonic cells of these bacteria was caused by the presence of >468.8 μg/mL of the extract, with growth inhibition ranging from 87.4 ± 0.0046% to 96.6 ± 0.0136% for A. baumannii ATCC 19606 and from 95.4 ± 0.0005% to 96.9 ± 0.0042% for A. baumannii ATCC BAA-3252 (statistically significant difference was noted at every extract concentrations except 30,000 μg/mL; p < 0.0001).

Techniques: Expressing