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upci scc 152  (ATCC)


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    Structured Review

    ATCC upci scc 152
    NSD2 silencing affects common and distinct pathways in HPV+ and HPV- HNSCC cell lines. A) RNA-seq was performed on 4 HPV- (UM-SCC-4, UM-SCC-6, UM-SCC-18, UM-SCC-19) and 3 HPV+ (UD-SCC-2, UM-SCC-104, UPCI: SCC-152) HNSCC cell lines transduced with shNSD2_A or scr control. Heatmap showing the DEGs in HPV- and HP+ HSNCC cell lines upon NSD2 silencing. K-means clustering has been applied with a number of clusters k = 9. The red-green scale represents the log2FC values, while the blue and white boxes indicate if the gene is statistically significant or not, respectively, for each of the two subgroups. Color legend indicates the number of each cluster. B) Gene Ontology (GO) analysis was performed on identified clusters. Bar plots show significant gene ontologies enriched in Cluster 2, 5 and 9. C) Total mRNA was extracted from the HPV+ HNSCC cell lines, UM-SCC-104 and <t>UPCI:SCC-152,</t> upon NSD2 silencing. Histograms showing the mRNA expression levels of a panel of differentiation markers analyzed by RT-qPCR and normalized on RPLP0. Values of at least three independent experiments are represented as fold changes on the scrambled control and expressed as means ± SD. One-sample t-test. D) Graph showing the ΔNp63α mRNA expression levels, analyzed by RT-qPCR and normalized on RPLP0. Values of at least two independent experiments, represented as fold changes on the scrambled control, are expressed as means ± SD. One-sample t-test. E) Immunoblot showing ΔNp63α protein levels in 4 HPV + HNSCC cell lines, upon NSD2 silencing. To optimize the visualization, both low exposure and high exposure acquisition are shown. Vinculin was used as loading control. The histogram on the right, shows the optical densitometric quantification of ΔNp63α bands normalized on Vinculin. F) Immunoblot showing NSD2 and ΔNp63α protein levels in 7 HPV- and 7 HPV+ HNSCC cell lines. Vinculin was used as loading control. G) Correlation between ΔNp63α and NSD2 protein levels measured by densitometric quantification of Western blot bands in HPV- (Spearman correlation coefficient r =−0,714; p-value = 0,08) and HPV+ HNSCC cell lines (Spearman correlation coefficient r = 0,924; p-value = 0,006). H) ALDH + cells were detected and quantified through the ALDEFLUOR assay in UPCI:SCC-152 cell line, upon NSD2-silencing, and acquired by FACS analysis. Treatment with DEAB inhibitor was used as negative control. The histogram shows the mean of four independent experiments ± SD. Paired t-test. I) Sphere formation assay was performed in UPC:SCC-152 cell line upon NSD2-silencing. Images were acquired 15 days post-plating; spheres > 70 μm in diameter were counted. The histogram shows the mean of two independent experiments ± SD. Unpaired t-test. *, p ≤ 0,05; **, p ≤ 0,01; ns, not significant (t-test).
    Upci Scc 152, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upci scc 152/product/ATCC
    Average 94 stars, based on 46 article reviews
    upci scc 152 - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "NSD2 upregulation is driven by high-risk HPV E6/E7 and disrupts epithelial differentiation in HPV-associated head and neck cancer"

    Article Title: NSD2 upregulation is driven by high-risk HPV E6/E7 and disrupts epithelial differentiation in HPV-associated head and neck cancer

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-025-03631-0

    NSD2 silencing affects common and distinct pathways in HPV+ and HPV- HNSCC cell lines. A) RNA-seq was performed on 4 HPV- (UM-SCC-4, UM-SCC-6, UM-SCC-18, UM-SCC-19) and 3 HPV+ (UD-SCC-2, UM-SCC-104, UPCI: SCC-152) HNSCC cell lines transduced with shNSD2_A or scr control. Heatmap showing the DEGs in HPV- and HP+ HSNCC cell lines upon NSD2 silencing. K-means clustering has been applied with a number of clusters k = 9. The red-green scale represents the log2FC values, while the blue and white boxes indicate if the gene is statistically significant or not, respectively, for each of the two subgroups. Color legend indicates the number of each cluster. B) Gene Ontology (GO) analysis was performed on identified clusters. Bar plots show significant gene ontologies enriched in Cluster 2, 5 and 9. C) Total mRNA was extracted from the HPV+ HNSCC cell lines, UM-SCC-104 and UPCI:SCC-152, upon NSD2 silencing. Histograms showing the mRNA expression levels of a panel of differentiation markers analyzed by RT-qPCR and normalized on RPLP0. Values of at least three independent experiments are represented as fold changes on the scrambled control and expressed as means ± SD. One-sample t-test. D) Graph showing the ΔNp63α mRNA expression levels, analyzed by RT-qPCR and normalized on RPLP0. Values of at least two independent experiments, represented as fold changes on the scrambled control, are expressed as means ± SD. One-sample t-test. E) Immunoblot showing ΔNp63α protein levels in 4 HPV + HNSCC cell lines, upon NSD2 silencing. To optimize the visualization, both low exposure and high exposure acquisition are shown. Vinculin was used as loading control. The histogram on the right, shows the optical densitometric quantification of ΔNp63α bands normalized on Vinculin. F) Immunoblot showing NSD2 and ΔNp63α protein levels in 7 HPV- and 7 HPV+ HNSCC cell lines. Vinculin was used as loading control. G) Correlation between ΔNp63α and NSD2 protein levels measured by densitometric quantification of Western blot bands in HPV- (Spearman correlation coefficient r =−0,714; p-value = 0,08) and HPV+ HNSCC cell lines (Spearman correlation coefficient r = 0,924; p-value = 0,006). H) ALDH + cells were detected and quantified through the ALDEFLUOR assay in UPCI:SCC-152 cell line, upon NSD2-silencing, and acquired by FACS analysis. Treatment with DEAB inhibitor was used as negative control. The histogram shows the mean of four independent experiments ± SD. Paired t-test. I) Sphere formation assay was performed in UPC:SCC-152 cell line upon NSD2-silencing. Images were acquired 15 days post-plating; spheres > 70 μm in diameter were counted. The histogram shows the mean of two independent experiments ± SD. Unpaired t-test. *, p ≤ 0,05; **, p ≤ 0,01; ns, not significant (t-test).
    Figure Legend Snippet: NSD2 silencing affects common and distinct pathways in HPV+ and HPV- HNSCC cell lines. A) RNA-seq was performed on 4 HPV- (UM-SCC-4, UM-SCC-6, UM-SCC-18, UM-SCC-19) and 3 HPV+ (UD-SCC-2, UM-SCC-104, UPCI: SCC-152) HNSCC cell lines transduced with shNSD2_A or scr control. Heatmap showing the DEGs in HPV- and HP+ HSNCC cell lines upon NSD2 silencing. K-means clustering has been applied with a number of clusters k = 9. The red-green scale represents the log2FC values, while the blue and white boxes indicate if the gene is statistically significant or not, respectively, for each of the two subgroups. Color legend indicates the number of each cluster. B) Gene Ontology (GO) analysis was performed on identified clusters. Bar plots show significant gene ontologies enriched in Cluster 2, 5 and 9. C) Total mRNA was extracted from the HPV+ HNSCC cell lines, UM-SCC-104 and UPCI:SCC-152, upon NSD2 silencing. Histograms showing the mRNA expression levels of a panel of differentiation markers analyzed by RT-qPCR and normalized on RPLP0. Values of at least three independent experiments are represented as fold changes on the scrambled control and expressed as means ± SD. One-sample t-test. D) Graph showing the ΔNp63α mRNA expression levels, analyzed by RT-qPCR and normalized on RPLP0. Values of at least two independent experiments, represented as fold changes on the scrambled control, are expressed as means ± SD. One-sample t-test. E) Immunoblot showing ΔNp63α protein levels in 4 HPV + HNSCC cell lines, upon NSD2 silencing. To optimize the visualization, both low exposure and high exposure acquisition are shown. Vinculin was used as loading control. The histogram on the right, shows the optical densitometric quantification of ΔNp63α bands normalized on Vinculin. F) Immunoblot showing NSD2 and ΔNp63α protein levels in 7 HPV- and 7 HPV+ HNSCC cell lines. Vinculin was used as loading control. G) Correlation between ΔNp63α and NSD2 protein levels measured by densitometric quantification of Western blot bands in HPV- (Spearman correlation coefficient r =−0,714; p-value = 0,08) and HPV+ HNSCC cell lines (Spearman correlation coefficient r = 0,924; p-value = 0,006). H) ALDH + cells were detected and quantified through the ALDEFLUOR assay in UPCI:SCC-152 cell line, upon NSD2-silencing, and acquired by FACS analysis. Treatment with DEAB inhibitor was used as negative control. The histogram shows the mean of four independent experiments ± SD. Paired t-test. I) Sphere formation assay was performed in UPC:SCC-152 cell line upon NSD2-silencing. Images were acquired 15 days post-plating; spheres > 70 μm in diameter were counted. The histogram shows the mean of two independent experiments ± SD. Unpaired t-test. *, p ≤ 0,05; **, p ≤ 0,01; ns, not significant (t-test).

    Techniques Used: RNA Sequencing, Transduction, Control, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Tube Formation Assay



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