atcc baa2464  (ATCC)

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Multiplex Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Intra Assay, Inter Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Multiplex Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Sequence alignments of amplicons generated in the 5‐plex qPCR assay. Primer sequences are indicated as red arrows. Variable nucleotide positions are highlighted in red, whereas conserved nucleotides are shown in blue. Accession numbers of GenBank of shown sequences are indicated if available. (a) cpn60 ‐amplicon generated using primers cpn60_for and cpn60_rev. The following colored arrows illustrate probe sequences of Brachyspira species: pink for B . suanatina , green for B. hyodysenteriae , and yellow for B . pilosicoli (b) nox‐amplicon generated using primers nox_for and nox_rev. The B . hampsonii ‐specific probe is indicated on the gene nox in orange

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Sequencing, Generated, Amplification

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Sequences of probes and primers used for the TaqMan multiplex qPCR assay. Channels for measuring the different fluorophores calculated PCR efficiencies measured in the linear range, and r 2 values are indicated. Borgström et al. ( 2017 ) represent locked nucleic acid bases (LNA) and MGB stands for minor groove binding probe

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Multiplex Assay, Binding Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: A concentration gradient of cpn60 primer is illustrated for B . pilosicoli , B . hyodysenteriae , B . hampsonii , and B . suanatina . A concentration of 400 nM cpn60 primer is optimal for detection of B . pilosicoli , B . hyodysenteriae , and B . suanatina , while detection of B . hampsonii with a nox ‐specific probe is not influenced by a gradient of cpn60 primer

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Concentration Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: A concentration gradient of nox primer is illustrated for B . pilosicoli , B . hyodysenteriae , B . hampsonii , and B . suanatina . A concentration of 400 nM nox primer is optimal for detection of B . hampsonii , while cpn60 ‐specific detection of B . pilosicoli , B . hyodysenteriae , and B . suanatina is not influenced by a gradient of nox primer

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Concentration Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Amplification, Control

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Limit of detection (LOD). Graphs illustrate the fraction of replicate samples with positive reads of dilution series at different concentrations (100 genome equivalents (GE), 50 GE, 20 GE, 10 GE, 5 GE, 1GE) in log scale. LOD is calculated at the relevant confidence level of 95% (green line). Data analysis was performed with GenEx ( http://www.multid.se ). The cut‐off threshold cycle value was 37. (a) LOD of B . hyodysenteriae : 17 genome equivalents (GE), (b) LOD of B . pilosicoli : 14 GE, (c) LOD of B . hampsonii : 16 GE, and (d) LOD of B . suanatina : 19 GE

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques:

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Intra Assay, Inter Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Standard curves of dilution series acquired by the qPCR in the dynamic range of 10 7 – 100 genome equivalents. (A) B . hyodysenteriae and B . suanatina have PCR efficiencies of 99% for both probes. (B) B . pilosicoli and B . hampsonii have PCR efficiencies of 97% and 93%, respectively

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques:

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Comparison of results of testing DNA samples from 26 different Brachyspira isolates and 477 clinical samples with four different PCR assays including a conventional PCR targeting at nox and 16S rDNA, a high resolution melting (HRM) assay targeting at nox , a multiplex qPCR targeting at 23S rDNA, and the newly developed 5‐plex qPCR targeting at cpn60 and nox . The 23S rDNA qPCR revealed false‐positive results for B . hampsonii (cross‐reaction either with B . hyodysenteriae or apathogenic probe) and B . suanatina . The novel 5‐plex qPCR can detect all four pathogenic Brachyspira species correctly

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Comparison, HRM Assay, Multiplex Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Multiplex Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

Article Snippet: B . hampsonii clade II , ATCC BAA2464 , positive in Channel Orange.

Techniques: Intra Assay, Inter Assay