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brachyspira culture density  (ATCC)


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    ATCC brachyspira culture density
    Brachyspira Culture Density, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brachyspira culture density/product/ATCC
    Average 92 stars, based on 10 article reviews
    brachyspira culture density - by Bioz Stars, 2026-02
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    18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

    Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

    Techniques: Multiplex Assay

    Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

    Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

    Techniques: Amplification, Control

    Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

    Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

    Techniques: Intra Assay, Inter Assay

    18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

    Article Snippet: B. hampsonii , ATCC BAA2463 , 50 , 32.88 , 33.21 , 0.29 , 0.87 , 32.38 , 33.01 , 0.63 , 1.89 , 32.74 , , , , , , , 32.67 , 0.64 , 1.95 , 32.96 , 0.27 , 0.83.

    Techniques: Multiplex Assay

    Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

    Article Snippet: B. hampsonii , ATCC BAA2463 , 50 , 32.88 , 33.21 , 0.29 , 0.87 , 32.38 , 33.01 , 0.63 , 1.89 , 32.74 , , , , , , , 32.67 , 0.64 , 1.95 , 32.96 , 0.27 , 0.83.

    Techniques: Amplification, Control

    Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

    Article Snippet: B. hampsonii , ATCC BAA2463 , 50 , 32.88 , 33.21 , 0.29 , 0.87 , 32.38 , 33.01 , 0.63 , 1.89 , 32.74 , , , , , , , 32.67 , 0.64 , 1.95 , 32.96 , 0.27 , 0.83.

    Techniques: Intra Assay, Inter Assay

    18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

    Article Snippet: B. hampsonii , ATCC BAA2463 , 10^6 , 17.69 , 17.91 , 0.30 , 1.67 , 17.80 , 18.08 , 0.26 , 1.44 , 18.05 , , , , , , , 18.38 , 0.40 , 2.16 , 18.12 , 0.24 , 1.31.

    Techniques: Multiplex Assay

    Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

    Article Snippet: B. hampsonii , ATCC BAA2463 , 10^6 , 17.69 , 17.91 , 0.30 , 1.67 , 17.80 , 18.08 , 0.26 , 1.44 , 18.05 , , , , , , , 18.38 , 0.40 , 2.16 , 18.12 , 0.24 , 1.31.

    Techniques: Amplification

    Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

    Journal: MicrobiologyOpen

    Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

    doi: 10.1002/mbo3.1169

    Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

    Article Snippet: B. hampsonii , ATCC BAA2463 , 10^6 , 17.69 , 17.91 , 0.30 , 1.67 , 17.80 , 18.08 , 0.26 , 1.44 , 18.05 , , , , , , , 18.38 , 0.40 , 2.16 , 18.12 , 0.24 , 1.31.

    Techniques: Intra Assay, Inter Assay