baa 2340  (ATCC)

Journal: Pharmaceutics

Article Title: Serendipitous Hinge Modulation Hypothetically Reprograms Caerin 1.1-LC Antibacterial Mechanism and Gram-Negative Selectivity

doi: 10.3390/pharmaceutics17111500

Figure Lengend Snippet: Peptide toxicity and in vivo antibacterial activity in E. coli -infected waxworms. For toxicity testing, both peptides were evaluated at concentrations of 1× MIC, 2× MIC, and 4× MIC. The green line (Caerin 1.1-LC 4× MIC) completely overlaps with the red line (Caerin 1.1-LC 2× MIC). An equivalent injection volume of PBS served as the growth control group. For the antibacterial study, an equal volume of bacterial suspension without peptide treatment was used as the untreated control group.

Article Snippet: E. coli ATCC BAA-2340 were grown in 25 mL of tryptic soy broth (TSB) for 2.5 h to the mid-log phase, then washed three times with a solution containing 5% TSB in 0.85% NaCl by centrifugation (3000× g , 10 min).

Techniques: In Vivo, Activity Assay, Infection, Injection, Control, Suspension

Journal: Pharmaceutics

Article Title: Serendipitous Hinge Modulation Hypothetically Reprograms Caerin 1.1-LC Antibacterial Mechanism and Gram-Negative Selectivity

doi: 10.3390/pharmaceutics17111500

Figure Lengend Snippet: Time–kill curves of Caerin 1.1-LC, Caerin 1.1-LC 7.11.19K, and Caerin 1.1-LC 7.11.19K.V(D)V(D) against E. coli (ATCC BAA-2340). The test duration was 120 min, with peptide concentrations ranging from 1× MIC to 4× MIC. Bacteria without peptide treatment were used as the growth control.

Article Snippet: E. coli ATCC BAA-2340 were grown in 25 mL of tryptic soy broth (TSB) for 2.5 h to the mid-log phase, then washed three times with a solution containing 5% TSB in 0.85% NaCl by centrifugation (3000× g , 10 min).

Techniques: Bacteria, Control

Journal: Pharmaceutics

Article Title: Serendipitous Hinge Modulation Hypothetically Reprograms Caerin 1.1-LC Antibacterial Mechanism and Gram-Negative Selectivity

doi: 10.3390/pharmaceutics17111500

Figure Lengend Snippet: Peptide influence on E. coli (ATCC BAA-2340) intracellular membrane permeability. Same volume of PBS treatment was set as growth control. Melittin (4× MIC, 16 μM) treatment was set as positive control. Error bars represent SD of nine independent replications.

Article Snippet: E. coli ATCC BAA-2340 were grown in 25 mL of tryptic soy broth (TSB) for 2.5 h to the mid-log phase, then washed three times with a solution containing 5% TSB in 0.85% NaCl by centrifugation (3000× g , 10 min).

Techniques: Membrane, Permeability, Control, Positive Control

Journal: Pharmaceutics

Article Title: Serendipitous Hinge Modulation Hypothetically Reprograms Caerin 1.1-LC Antibacterial Mechanism and Gram-Negative Selectivity

doi: 10.3390/pharmaceutics17111500

Figure Lengend Snippet: Fluorescence images of Caerin 1.1-LC 7.11.19K.V(D)V(D)-treated E. coli (ATCC BAA-2340). FM 4-64 (red) labels the plasma membrane, HOECHST 33,342 (blue) stains the cell nucleus, and FITC (green) is conjugated to our peptides to track their localisation. The scale bar is 25 µm.

Article Snippet: E. coli ATCC BAA-2340 were grown in 25 mL of tryptic soy broth (TSB) for 2.5 h to the mid-log phase, then washed three times with a solution containing 5% TSB in 0.85% NaCl by centrifugation (3000× g , 10 min).

Techniques: Fluorescence, Clinical Proteomics, Membrane

Journal: Pharmaceutics

Article Title: Serendipitous Hinge Modulation Hypothetically Reprograms Caerin 1.1-LC Antibacterial Mechanism and Gram-Negative Selectivity

doi: 10.3390/pharmaceutics17111500

Figure Lengend Snippet: Effect of Caerin 1.1-LC 7.11.19K.V(D)V(D) on cytoplasmic membrane depolarisation against E. coli (ATCC BAA-2340).

Article Snippet: E. coli ATCC BAA-2340 were grown in 25 mL of tryptic soy broth (TSB) for 2.5 h to the mid-log phase, then washed three times with a solution containing 5% TSB in 0.85% NaCl by centrifugation (3000× g , 10 min).

Techniques: Membrane

Journal: Pharmaceutics

Article Title: Serendipitous Hinge Modulation Hypothetically Reprograms Caerin 1.1-LC Antibacterial Mechanism and Gram-Negative Selectivity

doi: 10.3390/pharmaceutics17111500

Figure Lengend Snippet: Intracellular ATP level in E. coli (ATCC BAA-2340) with/without the treatment of Caerin 1.1-LC 7.11.19K.V(D)V(D).

Article Snippet: E. coli ATCC BAA-2340 were grown in 25 mL of tryptic soy broth (TSB) for 2.5 h to the mid-log phase, then washed three times with a solution containing 5% TSB in 0.85% NaCl by centrifugation (3000× g , 10 min).

Techniques: