Journal: Journal of Clinical Microbiology
Article Title: Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR
doi: 10.1128/JCM.02217-20
Figure Lengend Snippet: Results of the real-time PCR assay performed on Bartonella spp. and other bacterial species associated with culture-negative endocarditis
Article Snippet: Bartonella spp. were identified by PCR targeting various genes with amplicon sequencing ( ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial species/subsp. Strain designation(s) b Probe T m a (°C) or status Bartonella genus Bartonella henselae Bartonella quintana B. henselae BhTA-4, ATCC 49793 (87-66) 58–61 47–51 Negative B. quintana CIP 103739 (Toulouse), CIP 107027 (Fuller) 57–61 Negative 68–71 B. koehlerae ATCC 700693 57–59 Negative Negative B. vinsonii subsp. berkhoffii ATCC 51672 57 Negative Negative B. vinsonii subsp. arupensis ATCC BAA-2250 58 Negative Negative B. elizabethae ATCC 49927 43 Negative Negative B. washoensis ATCC BAA-2254 50 Negative Negative Coxiella burnetii ATCC VR-615 (Nine Mile phase I) Negative Negative Negative Tropheryma whipplei CNCM I-2202T (Twist-Marseille) Negative Negative Negative Legionella pneumophila subsp. pneumophila ATCC 33152 Negative Negative Negative Aggregatibacter aphrophilus ATCC 33389 Negative Negative Negative Aggregatibacter paraphrophilus NCTC 10557 Negative Negative Negative Aggregatibacter actinomycetemcomitans ATCC 700685 Negative Negative Negative Aggregatibacter segnis NCTC 10977 Negative Negative Negative Cardiobacterium hominis NCTC 10426 Negative Negative Negative Eikenella corrodens ATCC 23834 Negative Negative Negative Kingella kingae NCTC 10529 Negative Negative Negative Abiotrophia defectiva ATCC 49176 Negative Negative Negative Granulicatella adiacens ATCC 49175 Negative Negative Negative Granulicatella elegans ATCC 700633 Negative Negative Negative Gemella bergeriae ATCC 700627 Negative Negative Negative Gemella haemolysans ATCC 10379 Negative Negative Negative Gemella morbillorum NCTC 11323 Negative Negative Negative Gemella sanguinis ATCC 700632 Negative Negative Negative Open in a separate window a T m , melting temperature. b ATCC, American Type Culture Collection, USA; CIP, Collection of the Institute Pasteur, France; CNCM, Collection Nationale de Cultures de Microorganismes, Institut Pasteur, France; NCTC, National Collection of Type Cultures, Central Public Health Laboratory, United Kingdom.
Techniques: Real-time Polymerase Chain Reaction
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