Journal: Clinical Mass Spectrometry
Article Title: Direct detection of intact Klebsiella pneumoniae carbapenemase variants from cell lysates: Identification, characterization and clinical implications
doi: 10.1016/j.clinms.2020.07.001
Figure Lengend Snippet: Comparison of strains with and without KPC-4. Extracted ion chromatogram (EIC) centered on the y7+ fragment of KPC-4 from (a) DH5α carbapenem-susceptible E. coli lysate, (c) lysate from DH5α E. coli cells following transformation of a KPC + plasmid, and (e) cell lysate from KPC-4-producing K. pneumoniae, #104 from the CDC and FDA AR isolate Bank, from which the KPC + plasmid was isolated. The corresponding MS/MS spectra presenting (b) no evidence for KPC from the DH5α lysate, and fragment ions of KPC-4 from lysates of (d) DH5α KPC + and (f) the original KPC-producing isolate. MS spectra of the intact KPC-4 proteins from both (d) and (f) were also identified and are provided as supplemental information (Fig. S6).
Article Snippet: BAA-1899 , Klebsiella pneumoniae , ATCC , KPC-2 , KPC-2 , 3.2792.
Techniques: Comparison, Transformation Assay, Plasmid Preparation, Isolation, Tandem Mass Spectroscopy