Journal: Vaccines
Article Title: Feasibility of Using a Luminescence-Based Method to Determine Serum Bactericidal Activity against Neisseria gonorrhoeae
doi: 10.3390/vaccines7040191
Figure Lengend Snippet: Reduction of serum or SBA sensitivity or after sialylation with cytidine-5′-monophospho-N-acetylneuraminic acid (CMP-NANA). Mid-log broth subcultures of N. gonorrhoeae MS11 ( A ) or FA1090 ( B ) were incubated with/without the addition of 4 μg/mL CMP-NANA and samples taken to detect bacterial ATP. The P9-17 strain ( C ) was incubated under the same conditions with the addition of murine anti-P9-17 OMV anti-sera at a dilution of 1/64 to examine the effect of sialylation on SBA. Data are combined means + SD from three independent experiments; statistically significant differences in sensitivity were determined using multiple t -tests with the Holm–Šidák method applied ( A , B ) or a paired t -test ( C ). Values were reported as zero in experiments where bacteria numbers had increased in the presence of NHS. * p < 0.05; ** p < 0.01.
Article Snippet: N. gonorrhoeae strains FA1090 (ATCC 700825), MS11 (ATCC BAA-1833) and P9-17 [ ] were used in this study.
Techniques: Incubation, Bacteria