x 105 mecc staphylococcus aureus atcc baa  (ATCC)

Journal: Vaccines

Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens

doi: 10.3390/vaccines12010070

Figure Lengend Snippet: Survival percentages of ALPHA JECT vaccinated farmed Atlantic salmon after being challenged with M. viscosa . The experimental fish were ip and bath challenged with 10 6 CFU/dose and 10 6 CFU/mL of M. viscosa , respectively. The control group was not infected.

Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.

Techniques: Control, Infection

Journal: Vaccines

Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens

doi: 10.3390/vaccines12010070

Figure Lengend Snippet: Atlantic salmon blood cell populations identified by flow cytometry at 3, 7, and 14 dpc with M. viscosa . Differences were identified using one-way ANOVA (non-parametric) followed by Kruskal–Wallis post hoc tests at each sampling point. Asterisks (*) demonstrate a significant difference between groups ( p ≤ 0.05).

Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.

Techniques: Flow Cytometry, Sampling

Journal: Vaccines

Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens

doi: 10.3390/vaccines12010070

Figure Lengend Snippet: Spleen IgM + (B cells) detected by flow cytometry in ALPHA JECT-vaccinated Atlantic salmon at 3, 7, and 14 dpc with M. viscosa . Differences were identified by performing one-way ANOVA (non-parametric) followed by Kruskal–Wallis post hoc tests. At each time point, challenged groups are compared versus control (non-infected) fish. Asterisk (*) demonstrates a significant difference ( p ≤ 0.05) between groups.

Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.

Techniques: Flow Cytometry, Control, Infection

Journal: Vaccines

Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens

doi: 10.3390/vaccines12010070

Figure Lengend Snippet: IgM serum titers in Atlantic salmon at 3, 7, and 14 dpc with M. viscosa determined by indirect ELISA. Significant differences were identified using two-way ANOVA followed by Dunnett’s multiple comparisons test. The asterisk (*) indicates a significant difference ( p ≤ 0.0001) between the treatment and the control group at a particular sampling point.

Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.

Techniques: Indirect ELISA, Control, Sampling

Journal: Vaccines

Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens

doi: 10.3390/vaccines12010070

Figure Lengend Snippet: Blood cell populations in boosted Atlantic salmon analyzed by flow cytometry at 3, 7 and 14 days post bath challenge with M. viscosa . Significant differences were identified by performing one-way ANOVA (non-parametric) followed by Kruskal–Wallis post hoc tests. Asterisks (*) reveal a meaningful difference ( p ≤ 0.05) between experimental (i.e., boosted) and control fish (i.e., given a PBS injection with adjuvant).

Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.

Techniques: Flow Cytometry, Control, Injection, Adjuvant

Journal: Vaccines

Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens

doi: 10.3390/vaccines12010070

Figure Lengend Snippet: Blood cell populations in boosted Atlantic salmon identified by flow cytometry at 3, 7, and 14 days post ip challenge with M. viscosa . Significant differences were identified using one-way ANOVA (non-parametric) followed by Kruskal–Wallis post hoc tests. Asterisks (*) indicate a significant difference ( p ≤ 0.05) between experimental (i.e., boosted) and control fish (i.e., given a PBS injection with adjuvant).

Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.

Techniques: Flow Cytometry, Control, Injection, Adjuvant

Journal: Vaccines

Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens

doi: 10.3390/vaccines12010070

Figure Lengend Snippet: IgM + (B cells) in the spleen of boosted Atlantic salmon 3, 7 and 14 days after ip and bath challenges with M. viscosa. No significant differences ( p ≤ 0.05) were identified between the non-boosted and boosted treatments after performing one-way ANOVA (non-parametric) followed by Kruskal–Wallis post hoc tests.

Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.

Techniques: