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antibodies for phospho-eif2a (ser51, #af1237)  (Beyotime)


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    Structured Review

    Beyotime antibodies for phospho-eif2a (ser51, #af1237)
    Antibodies For Phospho Eif2a (Ser51, #Af1237), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AF1237/eif2%CE%B1+antibody/pm40460939-48-2-14
    Average 90 stars, based on 1 article reviews
    antibodies for phospho-eif2a (ser51, #af1237) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Nitrovin (difurazone), an antibacterial growth promoter, induces ROS-mediated paraptosis-like cell death by targeting thioredoxin reductase 1 (TrxR1).
    Article Snippet: Glioblastoma multiforme (GBM) is one of the most lethal malignant tumors in the human brain, with only a few chemotherapeutic drugs available after surgery.. Nitrovin (difurazone) is widely used as an antibacterial growth promoter in livestock.. Here, we reported that nitrovin might be a potential anticancer lead.

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: ISR inhibition reverses pancreatic β-cell failure in Wolfram syndrome models
    Article Snippet: Primary antibodies were listed as below: β-actin (Beyotime, AF5001, 1:5000), PERK (CellSignalingTechnology, 3192S, 1:1000), eIF2α (Beyotime, AF6771, 1:1000), P-eIF2α (Beyotime, AF1237, 1:1000), ATF4 (Proteintech, 10835-1-AP, 1:500).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice
    Article Snippet: College of Medicine, Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China; Innovation Center of Nursing Research, Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu 610041, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China

    Western Blot:

    Article Title: Nitrovin (difurazone), an antibacterial growth promoter, induces ROS-mediated paraptosis-like cell death by targeting thioredoxin reductase 1 (TrxR1).
    Article Snippet: Glioblastoma multiforme (GBM) is one of the most lethal malignant tumors in the human brain, with only a few chemotherapeutic drugs available after surgery.. Nitrovin (difurazone) is widely used as an antibacterial growth promoter in livestock.. Here, we reported that nitrovin might be a potential anticancer lead.

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: ISR inhibition reverses pancreatic β-cell failure in Wolfram syndrome models
    Article Snippet: Primary antibodies were listed as below: β-actin (Beyotime, AF5001, 1:5000), PERK (CellSignalingTechnology, 3192S, 1:1000), eIF2α (Beyotime, AF6771, 1:1000), P-eIF2α (Beyotime, AF1237, 1:1000), ATF4 (Proteintech, 10835-1-AP, 1:500).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice
    Article Snippet: College of Medicine, Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China; Innovation Center of Nursing Research, Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu 610041, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China

    Expressing:

    Article Title: Nitrovin (difurazone), an antibacterial growth promoter, induces ROS-mediated paraptosis-like cell death by targeting thioredoxin reductase 1 (TrxR1).
    Article Snippet: Glioblastoma multiforme (GBM) is one of the most lethal malignant tumors in the human brain, with only a few chemotherapeutic drugs available after surgery.. Nitrovin (difurazone) is widely used as an antibacterial growth promoter in livestock.. Here, we reported that nitrovin might be a potential anticancer lead.

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: ISR inhibition reverses pancreatic β-cell failure in Wolfram syndrome models
    Article Snippet: Primary antibodies were listed as below: β-actin (Beyotime, AF5001, 1:5000), PERK (CellSignalingTechnology, 3192S, 1:1000), eIF2α (Beyotime, AF6771, 1:1000), P-eIF2α (Beyotime, AF1237, 1:1000), ATF4 (Proteintech, 10835-1-AP, 1:500).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice
    Article Snippet: College of Medicine, Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China; Innovation Center of Nursing Research, Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu 610041, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China

    Labeling:

    Article Title: Nitrovin (difurazone), an antibacterial growth promoter, induces ROS-mediated paraptosis-like cell death by targeting thioredoxin reductase 1 (TrxR1).
    Article Snippet: Glioblastoma multiforme (GBM) is one of the most lethal malignant tumors in the human brain, with only a few chemotherapeutic drugs available after surgery.. Nitrovin (difurazone) is widely used as an antibacterial growth promoter in livestock.. Here, we reported that nitrovin might be a potential anticancer lead.

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: ISR inhibition reverses pancreatic β-cell failure in Wolfram syndrome models
    Article Snippet: Primary antibodies were listed as below: β-actin (Beyotime, AF5001, 1:5000), PERK (CellSignalingTechnology, 3192S, 1:1000), eIF2α (Beyotime, AF6771, 1:1000), P-eIF2α (Beyotime, AF1237, 1:1000), ATF4 (Proteintech, 10835-1-AP, 1:500).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice
    Article Snippet: College of Medicine, Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China; Innovation Center of Nursing Research, Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu 610041, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China

    Immunostaining:

    Article Title: Nitrovin (difurazone), an antibacterial growth promoter, induces ROS-mediated paraptosis-like cell death by targeting thioredoxin reductase 1 (TrxR1).
    Article Snippet: Glioblastoma multiforme (GBM) is one of the most lethal malignant tumors in the human brain, with only a few chemotherapeutic drugs available after surgery.. Nitrovin (difurazone) is widely used as an antibacterial growth promoter in livestock.. Here, we reported that nitrovin might be a potential anticancer lead.

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: ISR inhibition reverses pancreatic β-cell failure in Wolfram syndrome models
    Article Snippet: Primary antibodies were listed as below: β-actin (Beyotime, AF5001, 1:5000), PERK (CellSignalingTechnology, 3192S, 1:1000), eIF2α (Beyotime, AF6771, 1:1000), P-eIF2α (Beyotime, AF1237, 1:1000), ATF4 (Proteintech, 10835-1-AP, 1:500).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice
    Article Snippet: College of Medicine, Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China; Innovation Center of Nursing Research, Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu 610041, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China

    Two Tailed Test:

    Article Title: Nitrovin (difurazone), an antibacterial growth promoter, induces ROS-mediated paraptosis-like cell death by targeting thioredoxin reductase 1 (TrxR1).
    Article Snippet: Glioblastoma multiforme (GBM) is one of the most lethal malignant tumors in the human brain, with only a few chemotherapeutic drugs available after surgery.. Nitrovin (difurazone) is widely used as an antibacterial growth promoter in livestock.. Here, we reported that nitrovin might be a potential anticancer lead.

    Article Title: Squalene epoxidase promotes paraquat-induced pulmonary toxicity through endoplasmic reticulum-mediated ferroptosis.
    Article Snippet: Antibodies for phospho-eIF2a (Ser51, #AF1237), eIF2a (#AG1813), and phospho-PERK (Thr982, #AF5902) were obtained from Beyotime (Shanghai, China).

    Article Title: Blueberry extract attenuates DSS-induced inflammatory bowel disease in mice through inhibiting ER stress-mediated colonic apoptosis in mice.
    Article Snippet: Inflammatory bowel disease (IBD) is a chronic, debilitating condition with limited therapeutic options.. Dietary components like blueberries have emerged as potential modulators of inflammation and tissue repair in gastrointestinal diseases.. This study investigated endoplasmic reticulum (ER) stress-mediated apoptosis mediated protective effects of blueberries in ameliorating dextran sulfate sodium (DSS)induced IBD.

    Article Title: Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression
    Article Snippet: Mouse monoclonal anti-PEDV-N and anti-S antibodies were previously generated in the lab. Rabbit polyclonal anti-phospho-PERK (AF5902), rabbit polyclonal anti-eIF2α (AF6771), rabbit monoclonal anti-phospho-eIF2α (AF1237), rabbit monoclonal anti-PKR (AF2125) and rabbit monoclonal anti-phospho-PKR (AF1474) antibodies were purchased from Beyotime, Inc. (Shanghai, China).

    Article Title: ISR inhibition reverses pancreatic β-cell failure in Wolfram syndrome models
    Article Snippet: Primary antibodies were listed as below: β-actin (Beyotime, AF5001, 1:5000), PERK (CellSignalingTechnology, 3192S, 1:1000), eIF2α (Beyotime, AF6771, 1:1000), P-eIF2α (Beyotime, AF1237, 1:1000), ATF4 (Proteintech, 10835-1-AP, 1:500).

    Article Title: Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release.. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR).. However, how UPR is directly regulated by PEDV E protein remains elusive.

    Article Title: Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice
    Article Snippet: College of Medicine, Henan Engineering Research Center of Funiu Mountain’s Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China; Innovation Center of Nursing Research, Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu 610041, China; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China



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    Long-time exposure to cereulide triggers ER stress and induces apoptosis of liver and kidney cells in mice. ( A , B ) The mRNA levels of XBP1 , XBP1s , IRE1α , ATF4 and BiP were detected in liver and kidney tissues by RT-qPCR. Data are shown as the mean ± SD ( n = 5). ( C , D ) Western blot analysis for IRE1α, p-IRE1α, XBP1s, PERK, p-PERK, <t>p-eIF2α,</t> and CHOP in the liver and kidney tissues of mice exposed to cereulide (50 and 200 μg/kg). ( E , F ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (200 μg/kg). Arrows indicated apoptotic cells. Image magnification: ×400; scale bar = 50 µm. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group.
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    Long-time exposure to cereulide triggers ER stress and induces apoptosis of liver and kidney cells in mice. ( A , B ) The mRNA levels of XBP1 , XBP1s , IRE1α , ATF4 and BiP were detected in liver and kidney tissues by RT-qPCR. Data are shown as the mean ± SD ( n = 5). ( C , D ) Western blot analysis for IRE1α, p-IRE1α, XBP1s, PERK, p-PERK, <t>p-eIF2α,</t> and CHOP in the liver and kidney tissues of mice exposed to cereulide (50 and 200 μg/kg). ( E , F ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (200 μg/kg). Arrows indicated apoptotic cells. Image magnification: ×400; scale bar = 50 µm. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group.
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    Long-time exposure to cereulide triggers ER stress and induces apoptosis of liver and kidney cells in mice. ( A , B ) The mRNA levels of XBP1 , XBP1s , IRE1α , ATF4 and BiP were detected in liver and kidney tissues by RT-qPCR. Data are shown as the mean ± SD ( n = 5). ( C , D ) Western blot analysis for IRE1α, p-IRE1α, XBP1s, PERK, p-PERK, p-eIF2α, and CHOP in the liver and kidney tissues of mice exposed to cereulide (50 and 200 μg/kg). ( E , F ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (200 μg/kg). Arrows indicated apoptotic cells. Image magnification: ×400; scale bar = 50 µm. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group.

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: Long-time exposure to cereulide triggers ER stress and induces apoptosis of liver and kidney cells in mice. ( A , B ) The mRNA levels of XBP1 , XBP1s , IRE1α , ATF4 and BiP were detected in liver and kidney tissues by RT-qPCR. Data are shown as the mean ± SD ( n = 5). ( C , D ) Western blot analysis for IRE1α, p-IRE1α, XBP1s, PERK, p-PERK, p-eIF2α, and CHOP in the liver and kidney tissues of mice exposed to cereulide (50 and 200 μg/kg). ( E , F ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (200 μg/kg). Arrows indicated apoptotic cells. Image magnification: ×400; scale bar = 50 µm. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the control group.

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: Quantitative RT-PCR, Western Blot, TUNEL Assay

    Cereulide triggers apoptosis by mediating ER stress in HEK293T cells and HepaRG cells. ( A , B ) Cytotoxicity of cereulide in HepaRG and HEK293T cells. HepaRG and HEK293T cells were treated with different concentrations of cereulide for 24 h. Cell viability was then determined with CCK-8 ( n = 5). ( C ) Cell apoptosis resulting from cereulide treatment in HepaRG and HEK293T cells. HepaRG and HEK293T cells were evaluated with 0.3 and 1 ng/mL cereulide by flow cytometry. The data were analyzed using FlowJo software ( n = 3). ( D , E ) Representative transmission electron microscopy images of HepaRG and HEK293T cells treated with cereulide toxin. Cereulide treated HepaRG and HEK293T cells for 24 h, and observed by transmission electron microscope. M indicates mitochondria. Scale bars: left panel, 2 µm; right panel, 500 nm. ( F , G ) ER labeling. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h. Image magnification: ×200; scale bar = 100 µm. The cells were stained with ER-Tracker and observed under a fluorescence microscope at 587 nm excitation and 615 nm emission. ( H , I ) ER stress-related protein levels during cereulide exposure were evaluated in HepaRG and HEK293T cells. HepaRG and HEK293T cells were exposed to cereulide toxin (0, 0.3, 1 ng/mL) for 3, 6 and 24 h and Western blot analysis for BiP, ATF6, IRE1α, p-IRE1α, XBP1(s), PERK, p-PERK, p-eIF2α, CHOP. ( J ) IRE1α knock-down blocked cereulide to down-regulate XBP1s. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then exposed to cereulide (1 ng/mL) for 6 h. ( K ) IRE1α knock-down blocked cereulide to increase in apoptosis. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then evaluated with 1 ng/mL cereulide for 24 h by flow cytometry ( n = 3).

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: Cereulide triggers apoptosis by mediating ER stress in HEK293T cells and HepaRG cells. ( A , B ) Cytotoxicity of cereulide in HepaRG and HEK293T cells. HepaRG and HEK293T cells were treated with different concentrations of cereulide for 24 h. Cell viability was then determined with CCK-8 ( n = 5). ( C ) Cell apoptosis resulting from cereulide treatment in HepaRG and HEK293T cells. HepaRG and HEK293T cells were evaluated with 0.3 and 1 ng/mL cereulide by flow cytometry. The data were analyzed using FlowJo software ( n = 3). ( D , E ) Representative transmission electron microscopy images of HepaRG and HEK293T cells treated with cereulide toxin. Cereulide treated HepaRG and HEK293T cells for 24 h, and observed by transmission electron microscope. M indicates mitochondria. Scale bars: left panel, 2 µm; right panel, 500 nm. ( F , G ) ER labeling. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h. Image magnification: ×200; scale bar = 100 µm. The cells were stained with ER-Tracker and observed under a fluorescence microscope at 587 nm excitation and 615 nm emission. ( H , I ) ER stress-related protein levels during cereulide exposure were evaluated in HepaRG and HEK293T cells. HepaRG and HEK293T cells were exposed to cereulide toxin (0, 0.3, 1 ng/mL) for 3, 6 and 24 h and Western blot analysis for BiP, ATF6, IRE1α, p-IRE1α, XBP1(s), PERK, p-PERK, p-eIF2α, CHOP. ( J ) IRE1α knock-down blocked cereulide to down-regulate XBP1s. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then exposed to cereulide (1 ng/mL) for 6 h. ( K ) IRE1α knock-down blocked cereulide to increase in apoptosis. HepaRG cells were transfected with scramble siRNA as negative control (NC) or IRE1α siRNA for 24 h and then evaluated with 1 ng/mL cereulide for 24 h by flow cytometry ( n = 3).

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: CCK-8 Assay, Flow Cytometry, Software, Transmission Assay, Electron Microscopy, Microscopy, Labeling, Staining, Fluorescence, Western Blot, Transfection, Negative Control

    Cereulide induces ER Stress response by ROS production. ( A , B ) Cereulide causes an increase in ROS production. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h, the fluorescence of dichlorofluorescindiacetate (DCF) were detected at 488 nm excitation and 525 nm emission; n = 5. ( C , D ) Effects of ROS inhibitor on ROS production after cereulide treatment. HEK293T cells were pretreated with 5 mM NAC followed by treatment of 0.5 ng/mL cereulide for 2, 4, 24 h. Cytosolic ROS production measured by DHE labeling, n = 5. ( E , F ) HepaRG cells were pretreated with 7 mM NAC followed by treatment of 1 ng/mL cereulide for 2, 4, 24 h; n = 5. For C and E panels, image magnification: ×400; scale bar = 50 µm. ( G , H ) ROS inhibitor relieves ER stress caused by cereulide. According to the dosage described above, HepaRG and HEK293T cells were treated for 4 h and Western blot analysis for p-eIF2α, CHOP. ( I ) NAC treatment reduce the apoptosis caused by cereulide. HepaRG and HEK293T cells were treated as described above and then evaluated by flow cytometry; n = 3. For all panels, * p < 0.05 and ** p < 0.01 compared to the control cells; # p < 0.05 and ## p < 0.01 compared to NAC treated cells.

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: Cereulide induces ER Stress response by ROS production. ( A , B ) Cereulide causes an increase in ROS production. HepaRG and HEK293T cells were treated with 0, 0.3, 1 ng/mL cereulide for 24 h, the fluorescence of dichlorofluorescindiacetate (DCF) were detected at 488 nm excitation and 525 nm emission; n = 5. ( C , D ) Effects of ROS inhibitor on ROS production after cereulide treatment. HEK293T cells were pretreated with 5 mM NAC followed by treatment of 0.5 ng/mL cereulide for 2, 4, 24 h. Cytosolic ROS production measured by DHE labeling, n = 5. ( E , F ) HepaRG cells were pretreated with 7 mM NAC followed by treatment of 1 ng/mL cereulide for 2, 4, 24 h; n = 5. For C and E panels, image magnification: ×400; scale bar = 50 µm. ( G , H ) ROS inhibitor relieves ER stress caused by cereulide. According to the dosage described above, HepaRG and HEK293T cells were treated for 4 h and Western blot analysis for p-eIF2α, CHOP. ( I ) NAC treatment reduce the apoptosis caused by cereulide. HepaRG and HEK293T cells were treated as described above and then evaluated by flow cytometry; n = 3. For all panels, * p < 0.05 and ** p < 0.01 compared to the control cells; # p < 0.05 and ## p < 0.01 compared to NAC treated cells.

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: Fluorescence, Labeling, Western Blot, Flow Cytometry

    NaB relieves cereulide-induced liver and kidney damage by inhibiting ER stress. ( A ) Representative images of H&E-stained liver and kidney tissues from cereulide + NaB-treated mice and cereulide-treated mice (×200 magnification). ( B , C ) The mRNA levels of TNF-α, IL-6 and IL-10 were detected in liver and kidney tissues by RT-qPCR, n = 5. ( D , E ) Western blot analysis for XBP1(s), p-eIF2α, CHOP and Caspase3 in the liver and kidney tissues of mice that exposed to cereulide (50 μg/kg) were treated with NaB (100 mg/kg) for 28 days. ( F , G ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (50 μg/kg) and cereulide + NaB (100 mg/kg) (×400 magnification). Arrows indicated apoptotic cells. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05 and ** p < 0.01 compared to the control group; # p < 0.05 and ## p < 0.01 compared to cereulide + NaB group.

    Journal: International Journal of Molecular Sciences

    Article Title: Cereulide Exposure Caused Cytopathogenic Damages of Liver and Kidney in Mice

    doi: 10.3390/ijms22179148

    Figure Lengend Snippet: NaB relieves cereulide-induced liver and kidney damage by inhibiting ER stress. ( A ) Representative images of H&E-stained liver and kidney tissues from cereulide + NaB-treated mice and cereulide-treated mice (×200 magnification). ( B , C ) The mRNA levels of TNF-α, IL-6 and IL-10 were detected in liver and kidney tissues by RT-qPCR, n = 5. ( D , E ) Western blot analysis for XBP1(s), p-eIF2α, CHOP and Caspase3 in the liver and kidney tissues of mice that exposed to cereulide (50 μg/kg) were treated with NaB (100 mg/kg) for 28 days. ( F , G ) TUNEL analysis of apoptosis level in liver and kidney tissue of mice exposed to cereulide (50 μg/kg) and cereulide + NaB (100 mg/kg) (×400 magnification). Arrows indicated apoptotic cells. Data are presented as the mean ± SD ( n = 6). For all panels, * p < 0.05 and ** p < 0.01 compared to the control group; # p < 0.05 and ## p < 0.01 compared to cereulide + NaB group.

    Article Snippet: Antibodies against CHOP (AC532-L63F7), p-eIF2α (AF1237), XBP1s (AF8366), Phospho-PERK (AF5902) was purchased from Beyotime Biotech (Shanghai, China), anti-ATF6 (D262665) were purchased from BBI (Shanghai, China).

    Techniques: Staining, Quantitative RT-PCR, Western Blot, TUNEL Assay