Journal: Frontiers in Pharmacology
Article Title: Pinelliae rhizoma alleviated acute lung injury induced by lipopolysaccharide via suppressing endoplasmic reticulum stress-mediated NLRP3 inflammasome
doi: 10.3389/fphar.2022.883865
Figure Lengend Snippet: Validation of the effects of DHC, chrysin, and 7,8-DHF on inflammation-related proteins. RAW264.7 cells were exposed to LPS and treated with DHC, chrysin, or 7,8-DHF for 12 h. (A , B) The effects of DHC on the expressions of TRPV1, NLRP3, Caspase-1, p-NF-κB, Bip, and ATF4 were examined by Western blot ( n = 3). (C , D) The effects of chrysin on the expressions of ALOX12, p-IκBα, IκBα, p-NF-κB, NLRP3, Caspase-1, Bip, and ATF4 were detected by Western blot. ( n = 3). (E , F) Western blot was employed to detect the effects of 7,8-DHF on the expressions of IKKB, p-IκBα, IκBα, p-NF-κB, NLRP3 and Caspase-1 ( n = 3). *p < 0.05 , **p < 0.01 , ***p < 0.001 , ****p < 0.0001 vs. Control groups ; #p < 0.05 , ##p < 0.01 , ###p < 0.001 , ####p < 0.0001 vs. LPS-treated groups.
Article Snippet: Anti- ALOX12 (A02275) was purchased from Boster Biological Technology Co. Ltd. (Wuhan, China).
Techniques: Biomarker Discovery, Western Blot, Control