lrse st5  (ATCC)

Journal:

Article Title: RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria

doi:

Figure Lengend Snippet: Models of potential secondary structure of helix 9 in the 23S rRNA primary transcript of the following strains (asterisks indicate sequences obtained from the data bank, the other sequences were determined in our laboratory, and EMBL accession numbers are in parentheses): A, R. rubrum DSM 107 (AJ251267); B, R. sphaeroides WS8 (AJ251261); C, R. sphaeroides 17023 (AJ251260); D, R. capsulatus B10 (AJ251256); E, R. capsulatus 37b4 (AJ251255); F, R. capsulatus DSM 938* (reference 11); G, R. palustris 5D (AJ251262); H, B. japonicum 110* (reference 17); I, B. bacilliformis KC 584* (reference 22); J, B. henselae ATCC 49882 (AJ251257); K, S. fredii MSDJ 1536 (AJ251258); L, R. giardinii H152 (AJ251263); M, R. etli CFN 42 (AJ251265); R. etli Viking I (AJ251266); O, R. gallicum R602 (AJ251259), R. leguminosarum ATCC 10004 (AJ251264). (B to F) Rhodobacter group of helices. Boxes with highly conservative base pair occupation, specific for this group, are indicated. The differences between the sequences shown in panels E and F are in boldface letters. (G to P) Rhizobium-Bradyrhizobium group of helices. Boxes with highly conservative base pair occupation, specific for this group, are indicated. In panels G and H, sequences of high similarity around the putative deletion and/or insertion site are underlined. Arrows indicate the approximate positions of the RNase III processing sites as determined by RNA fragment length estimation (Table ​(Table4).4). Arrows on the left side of the helices indicate 5′-processing sites; arrows on the right side of the helices indicate 3′-processing sites. Filled arrowheads indicate primary processing sites; empty arrowheads indicate secondary processing sites.

Article Snippet: The results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain (lane nos. in Fig. ) % Transcript cleaved in vitro using: RNase III Ec with: RNase III Rc with: 130 mM KCl 250 mM KCl 130 mM KCl 250 mM KCl R. sphaeroides WS8 (21–25) 0 0 98 43 R. sphaeroides 17023 (56–58) 0 0 90 10 R. capsulatus B10 (46–50) 0 0 97 12 R. capsulatus 37b4 (6–10) 0 0 94 14 R. palustris 5D (1–5) 96 75 100 99 B. henselae ATCC 49882 (11–15) 97 70 99 96 S. fredii MSDJ 1536 (31–35) 2 1.5 100 100 R. giardinii H152 (26–30) 25 0 100 80 R. etli CFN42 (16–20) 10 0 99 99 R. etli Viking I (41–45) 80 0 98 99 R. gallicum R602 (36–40) 60 0 100 86 R. leguminosarum ATCC 10004 (51–55) 88 2 99 48 Open in a separate window a The reported values are the average of two experiments; the values did not deviate by more than 15%.

Techniques:

Journal:

Article Title: RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria

doi:

Figure Lengend Snippet: Summary of the approximate lengths of the helix 9 containing transcripts from the strains studied here and the approximate lengths of the fragments arising after in vitro cleavage of these transcripts by RNases III from R. capsulatus and E. coli a

Article Snippet: The results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain (lane nos. in Fig. ) % Transcript cleaved in vitro using: RNase III Ec with: RNase III Rc with: 130 mM KCl 250 mM KCl 130 mM KCl 250 mM KCl R. sphaeroides WS8 (21–25) 0 0 98 43 R. sphaeroides 17023 (56–58) 0 0 90 10 R. capsulatus B10 (46–50) 0 0 97 12 R. capsulatus 37b4 (6–10) 0 0 94 14 R. palustris 5D (1–5) 96 75 100 99 B. henselae ATCC 49882 (11–15) 97 70 99 96 S. fredii MSDJ 1536 (31–35) 2 1.5 100 100 R. giardinii H152 (26–30) 25 0 100 80 R. etli CFN42 (16–20) 10 0 99 99 R. etli Viking I (41–45) 80 0 98 99 R. gallicum R602 (36–40) 60 0 100 86 R. leguminosarum ATCC 10004 (51–55) 88 2 99 48 Open in a separate window a The reported values are the average of two experiments; the values did not deviate by more than 15%.

Techniques: In Vitro

Journal:

Article Title: RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria

doi:

Figure Lengend Snippet: Percentage of sequence identity found by comparison of the first 30 bp of the helix 9 of 23S rRNA of various bacterial strains a

Article Snippet: The results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain (lane nos. in Fig. ) % Transcript cleaved in vitro using: RNase III Ec with: RNase III Rc with: 130 mM KCl 250 mM KCl 130 mM KCl 250 mM KCl R. sphaeroides WS8 (21–25) 0 0 98 43 R. sphaeroides 17023 (56–58) 0 0 90 10 R. capsulatus B10 (46–50) 0 0 97 12 R. capsulatus 37b4 (6–10) 0 0 94 14 R. palustris 5D (1–5) 96 75 100 99 B. henselae ATCC 49882 (11–15) 97 70 99 96 S. fredii MSDJ 1536 (31–35) 2 1.5 100 100 R. giardinii H152 (26–30) 25 0 100 80 R. etli CFN42 (16–20) 10 0 99 99 R. etli Viking I (41–45) 80 0 98 99 R. gallicum R602 (36–40) 60 0 100 86 R. leguminosarum ATCC 10004 (51–55) 88 2 99 48 Open in a separate window a The reported values are the average of two experiments; the values did not deviate by more than 15%.

Techniques: Sequencing

Journal:

Article Title: RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria

doi:

Figure Lengend Snippet: GC content of helix 9 of 23S rRNA in comparison with the overall GC content of complete rrn operons or 23S rRNA

Article Snippet: The results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain (lane nos. in Fig. ) % Transcript cleaved in vitro using: RNase III Ec with: RNase III Rc with: 130 mM KCl 250 mM KCl 130 mM KCl 250 mM KCl R. sphaeroides WS8 (21–25) 0 0 98 43 R. sphaeroides 17023 (56–58) 0 0 90 10 R. capsulatus B10 (46–50) 0 0 97 12 R. capsulatus 37b4 (6–10) 0 0 94 14 R. palustris 5D (1–5) 96 75 100 99 B. henselae ATCC 49882 (11–15) 97 70 99 96 S. fredii MSDJ 1536 (31–35) 2 1.5 100 100 R. giardinii H152 (26–30) 25 0 100 80 R. etli CFN42 (16–20) 10 0 99 99 R. etli Viking I (41–45) 80 0 98 99 R. gallicum R602 (36–40) 60 0 100 86 R. leguminosarum ATCC 10004 (51–55) 88 2 99 48 Open in a separate window a The reported values are the average of two experiments; the values did not deviate by more than 15%.

Techniques:

Journal:

Article Title: RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria

doi:

Figure Lengend Snippet: Percentage of in vitro-cleaved transcripts containing helix 9 of 23S rRNA using E. coli and R. capsulatus RNases III at 130 mM and 250 mM KCl a

Article Snippet: The results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain (lane nos. in Fig. ) % Transcript cleaved in vitro using: RNase III Ec with: RNase III Rc with: 130 mM KCl 250 mM KCl 130 mM KCl 250 mM KCl R. sphaeroides WS8 (21–25) 0 0 98 43 R. sphaeroides 17023 (56–58) 0 0 90 10 R. capsulatus B10 (46–50) 0 0 97 12 R. capsulatus 37b4 (6–10) 0 0 94 14 R. palustris 5D (1–5) 96 75 100 99 B. henselae ATCC 49882 (11–15) 97 70 99 96 S. fredii MSDJ 1536 (31–35) 2 1.5 100 100 R. giardinii H152 (26–30) 25 0 100 80 R. etli CFN42 (16–20) 10 0 99 99 R. etli Viking I (41–45) 80 0 98 99 R. gallicum R602 (36–40) 60 0 100 86 R. leguminosarum ATCC 10004 (51–55) 88 2 99 48 Open in a separate window a The reported values are the average of two experiments; the values did not deviate by more than 15%.

Techniques: In Vitro

Journal:

Article Title: RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria

doi:

Figure Lengend Snippet: In vitro processing of transcripts containing helix 9 of 23S rRNA by R. capsulatus (Rc) and E. coli (Ec) RNase III at low and high monovalent ion concentrations. L, 130 mM KCl; H, 250 mM KCl; C, uncleaved substrate. Rp, R. palustris; Rc, R. capsulatus; Bh, B. henselae; Rhe, R. etli; Rs, R. sphaeroides; Rhgi, R. giardinii; Sf, S. fredi; Rhga, R. gallicum; Rhl, R. leguminosarum.

Article Snippet: The results are shown in Table . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain (lane nos. in Fig. ) % Transcript cleaved in vitro using: RNase III Ec with: RNase III Rc with: 130 mM KCl 250 mM KCl 130 mM KCl 250 mM KCl R. sphaeroides WS8 (21–25) 0 0 98 43 R. sphaeroides 17023 (56–58) 0 0 90 10 R. capsulatus B10 (46–50) 0 0 97 12 R. capsulatus 37b4 (6–10) 0 0 94 14 R. palustris 5D (1–5) 96 75 100 99 B. henselae ATCC 49882 (11–15) 97 70 99 96 S. fredii MSDJ 1536 (31–35) 2 1.5 100 100 R. giardinii H152 (26–30) 25 0 100 80 R. etli CFN42 (16–20) 10 0 99 99 R. etli Viking I (41–45) 80 0 98 99 R. gallicum R602 (36–40) 60 0 100 86 R. leguminosarum ATCC 10004 (51–55) 88 2 99 48 Open in a separate window a The reported values are the average of two experiments; the values did not deviate by more than 15%.

Techniques: In Vitro