Journal: Inflammatory bowel diseases

Article Title: Knockdown of Smad7 With a Specific Antisense Oligonucleotide Attenuates Colitis and Colitis-Driven Colonic Fibrosis in Mice.

doi: 10.1093/ibd/izy062

Figure Lengend Snippet: FIGURE 4. Treatment of colitic mice with Smad7 antisense oligonucleotide reduces collagen deposition and fibrosis. A, Representative photomicro- graphs of colon sections taken from TNBS-treated mice given Smad7 AS or control (sense) oligonucleotide and stained with Masson’s trichrome (left panels), collagen type I alpha 1 antibody (right panels), or relative isotype IgG (middle panels). B, Fibrosis microscopic score of colonic sections taken from TNBS-treated mice given Smad7 AS or control (sense) oligonucleotide. Data indicate mean ± SEM of 3 independent experiments in which 18 mice per group were analyzed.

Article Snippet: The slides were then incubated with a rabbit polyclonal antibody directed against collagen type I alpha 1 (final dilution 1:20 000; Novus biologicals, Littleton, CO, USA) or with a rabbit polyclonal Smad7 antibody (final dilution 1:300; Biorbyt, Cambridgeshire, UK) at room temperature for 30 minutes.

Techniques: Control, Staining

Journal: Inflammatory bowel diseases

Article Title: Knockdown of Smad7 With a Specific Antisense Oligonucleotide Attenuates Colitis and Colitis-Driven Colonic Fibrosis in Mice.

doi: 10.1093/ibd/izy062

Figure Lengend Snippet: FIGURE 6. Smad7 antisense oligonucleotide reduces colonic fibrosis in mice receiving long-term treatment with TNBS. A, Representative immunob- lots showing Smad7 and ERK1/2 in proteins extracted from mice receiving rectal administration of TNBS for 12 weeks and given Smad7 antisense oligonucleotide (AS) or control (sense) oligonucleotide starting from week 9 until the end of the study (week 12). B, Representative photomicro- graphs of H&E-stained colon sections of mice treated as indicated above. C, Representative photomicrographs of colon sections taken from TNBS- treated mice given Smad7 AS or control (sense) oligonucleotide and stained with Masson’s trichrome (left panels), collagen type I alpha 1 antibody (right panels), or relative isotype IgG (middle panels). D, Fibrosis microscopic score of colonic sections taken from TNBS-treated mice given Smad7 AS or control (sense) oligonucleotide. Each point represents the value of the fibrosis score in a single mouse, and the horizontal bar indicates the median value. E, Soluble collagen in colon samples was analyzed by Sircol assay. Data are shown as mean ±SD.

Article Snippet: The slides were then incubated with a rabbit polyclonal antibody directed against collagen type I alpha 1 (final dilution 1:20 000; Novus biologicals, Littleton, CO, USA) or with a rabbit polyclonal Smad7 antibody (final dilution 1:300; Biorbyt, Cambridgeshire, UK) at room temperature for 30 minutes.

Techniques: Control, Staining

Journal: Inflammatory bowel diseases

Article Title: Knockdown of Smad7 With a Specific Antisense Oligonucleotide Attenuates Colitis and Colitis-Driven Colonic Fibrosis in Mice.

doi: 10.1093/ibd/izy062

Figure Lengend Snippet: FIGURE 7. Smad7 AS oligonucleotide reduces DSS-induced colitis-driven colonic fibrosis. A, Representative immunoblots showing Smad7 and ERK1/2 in total proteins extracted from the colons of control mice, DSS-treated mice, and DSS-treated mice given Smad7 AS or control (sense) oligo- nucleotide. Right panel, Quantitative analysis of Smad7/ERK1/2 protein ratio in extracts of colon specimens measured by densitometry scanning of immunoblots. Values are expressed in arbitrary units (a.u.). Each point represents the value of the Smad7/ERK1/2 protein ratio in a single mouse, and the horizontal bar indicates the median value. B, Representative photomicrographs of colon sections taken from control and DSS-treated mice given Smad7 AS or control (sense) oligonucleotide and stained with collagen type I alpha 1 antibody (lower panels) or relative isotype IgG (upper panels).

Article Snippet: The slides were then incubated with a rabbit polyclonal antibody directed against collagen type I alpha 1 (final dilution 1:20 000; Novus biologicals, Littleton, CO, USA) or with a rabbit polyclonal Smad7 antibody (final dilution 1:300; Biorbyt, Cambridgeshire, UK) at room temperature for 30 minutes.

Techniques: Western Blot, Control, Staining