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j winton  (ATCC)


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    ATCC j winton
    J Winton, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/61307/pm34028055-62-125-141?v=ATCC
    Average 90 stars, based on 2 article reviews
    j winton - by Bioz Stars, 2026-08
    90/100 stars

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    Formation of polyubiquitinated proteins in FA-treated H460 human lung cells. A: Representative images of cells stained for DNA (DAPI), S-phase cells (EdU), and polyubiquitinated proteins (FK2 antibody). Cells were treated with FA for 3 hours in complete medium. B: Immunostaining for K48-Ub in FA-treated (3 hours) and heat-shocked cells. C: Cell viability and colony formation after FA treatments. Viability was measured at 72 hours after FA exposure. D: Formation of K48-Ub by FA and MG132 (5 μmol/L). Tubulin is used as a loading control. E: Western blot analysis for K63-specific polyubiquitination of soluble proteins in cells treated with FA and the deubiquitinase inhibitor G5. F: Western blot analysis for K63-Ub in the insoluble cellular proteins. Fibrillarin was used as a loading control. Data are expressed as means ± SD. n = 2 independent experiments, each including three dishes (clonogenic survival) or four wells (viability assay) per dose (C). EdU, 5-ethynyl-2′-deoxyuridine; FA, formaldehyde; K48-Ub, K48-linked polyubiquitin; K63-Ub, K63-polyubiquitination.

    Journal: The American Journal of Pathology

    Article Title: Formaldehyde Is a Potent Proteotoxic Stressor Causing Rapid Heat Shock Transcription Factor 1 Activation and Lys48-Linked Polyubiquitination of Proteins

    doi: 10.1016/j.ajpath.2016.06.022

    Figure Lengend Snippet: Formation of polyubiquitinated proteins in FA-treated H460 human lung cells. A: Representative images of cells stained for DNA (DAPI), S-phase cells (EdU), and polyubiquitinated proteins (FK2 antibody). Cells were treated with FA for 3 hours in complete medium. B: Immunostaining for K48-Ub in FA-treated (3 hours) and heat-shocked cells. C: Cell viability and colony formation after FA treatments. Viability was measured at 72 hours after FA exposure. D: Formation of K48-Ub by FA and MG132 (5 μmol/L). Tubulin is used as a loading control. E: Western blot analysis for K63-specific polyubiquitination of soluble proteins in cells treated with FA and the deubiquitinase inhibitor G5. F: Western blot analysis for K63-Ub in the insoluble cellular proteins. Fibrillarin was used as a loading control. Data are expressed as means ± SD. n = 2 independent experiments, each including three dishes (clonogenic survival) or four wells (viability assay) per dose (C). EdU, 5-ethynyl-2′-deoxyuridine; FA, formaldehyde; K48-Ub, K48-linked polyubiquitin; K63-Ub, K63-polyubiquitination.

    Article Snippet: Primary antibodies for K48-linked polyubiquitin (05-61307; Millipore), K63-linked polyubiquitin (BML-PW0600; Enzo, Farmingdale, NY), phospho-S326-HSF1 (ab76076; Abcam), HSF1 (4356; Cell Signaling Technology), FK2 (BML-PW8810; Enzo), HSP72/73 (HSP01, Millipore) were all used at 1:200 dilution.

    Techniques: Staining, Immunostaining, Western Blot, Viability Assay

    Polyubiquitination of proteins in response to FA in primary human cells. A: Epifluorescence images of IMR90 and HBE cells stained for DNA (DAPI) and polyubiquitinated proteins (FK2 antibody). B: Viability of IMR90 cells treated with 300 μmol/L FA for 0 to 3 hours and HBE cells treated with 0 to 300 μmol/L FA for 1 hour. Cell viability was measured at 72 hours after FA removal. C: Western blots for linkage-specific polyubiquitination in the soluble (top panel) and insoluble (bottom panel) proteins. Tubulin and fibrillarin were used as loading controls. D: Confocal images of IMR90 and HBE cells immunostained for polyubiquitinated proteins with FK2 antibody. Data are expressed as means ± SD. n = 3 (IMR90) or 2 experiments (HBE), each including four wells per dose (B). FA, formaldehyde; HBE, human bronchial epithelial; K48-Ub, K48-linked polyubiquitin; K63-Ub, K63-polyubiquitin.

    Journal: The American Journal of Pathology

    Article Title: Formaldehyde Is a Potent Proteotoxic Stressor Causing Rapid Heat Shock Transcription Factor 1 Activation and Lys48-Linked Polyubiquitination of Proteins

    doi: 10.1016/j.ajpath.2016.06.022

    Figure Lengend Snippet: Polyubiquitination of proteins in response to FA in primary human cells. A: Epifluorescence images of IMR90 and HBE cells stained for DNA (DAPI) and polyubiquitinated proteins (FK2 antibody). B: Viability of IMR90 cells treated with 300 μmol/L FA for 0 to 3 hours and HBE cells treated with 0 to 300 μmol/L FA for 1 hour. Cell viability was measured at 72 hours after FA removal. C: Western blots for linkage-specific polyubiquitination in the soluble (top panel) and insoluble (bottom panel) proteins. Tubulin and fibrillarin were used as loading controls. D: Confocal images of IMR90 and HBE cells immunostained for polyubiquitinated proteins with FK2 antibody. Data are expressed as means ± SD. n = 3 (IMR90) or 2 experiments (HBE), each including four wells per dose (B). FA, formaldehyde; HBE, human bronchial epithelial; K48-Ub, K48-linked polyubiquitin; K63-Ub, K63-polyubiquitin.

    Article Snippet: Primary antibodies for K48-linked polyubiquitin (05-61307; Millipore), K63-linked polyubiquitin (BML-PW0600; Enzo, Farmingdale, NY), phospho-S326-HSF1 (ab76076; Abcam), HSF1 (4356; Cell Signaling Technology), FK2 (BML-PW8810; Enzo), HSP72/73 (HSP01, Millipore) were all used at 1:200 dilution.

    Techniques: Staining, Western Blot