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B16f10, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell viability assay. <t>B16F10</t> cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.
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Cell viability assay. <t>B16F10</t> cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.
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Cell viability assay. <t>B16F10</t> cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.
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Cell viability assay. <t>B16F10</t> cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.
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Cell viability assay. <t>B16F10</t> cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.
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Cell viability assay. <t>B16F10</t> cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.
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Cell viability assay. B16F10 cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.

Journal: International Journal of Molecular Sciences

Article Title: In Vitro Characterization of Centella asiatica Extracellular Vesicles and Their Skin Repair Effects in a UVB-Irradiated Mouse Model

doi: 10.3390/ijms26188982

Figure Lengend Snippet: Cell viability assay. B16F10 cells were treated with different concentrations of C. asiatica EVs. After 24 h of treatment, cell viability was measured by the MTT assay method. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group.

Article Snippet: The following cell lines were used in the study: - B16F10 (mouse melanoma, BCRC #60031, ATCC CRL6475) was maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (Penicillin and streptomycin, Pen-Strep, Cat. #15070063, Gibco, Thermo Fisher Scientific, Vantaa, Finland).

Techniques: Viability Assay, MTT Assay, Control

Intracellular ROS level. B16F10 cells were pretreated with various concentrations of C. asiatica EVs, Trolox (0.5 mg/mL, positive control), or left untreated as a control for 24 h. The cells were then incubated with 24 mM H 2 O 2 and DCFH-DA. The fluorescence intensities of DCF were measured to calculate the ROS levels. Data are presented as mean ± SD ( n = 3). Statistical analysis was calculated by one-way ANOVA with Dunnett’s multiple comparisons test, compared to the control group. **** p < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: In Vitro Characterization of Centella asiatica Extracellular Vesicles and Their Skin Repair Effects in a UVB-Irradiated Mouse Model

doi: 10.3390/ijms26188982

Figure Lengend Snippet: Intracellular ROS level. B16F10 cells were pretreated with various concentrations of C. asiatica EVs, Trolox (0.5 mg/mL, positive control), or left untreated as a control for 24 h. The cells were then incubated with 24 mM H 2 O 2 and DCFH-DA. The fluorescence intensities of DCF were measured to calculate the ROS levels. Data are presented as mean ± SD ( n = 3). Statistical analysis was calculated by one-way ANOVA with Dunnett’s multiple comparisons test, compared to the control group. **** p < 0.0001.

Article Snippet: The following cell lines were used in the study: - B16F10 (mouse melanoma, BCRC #60031, ATCC CRL6475) was maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (Penicillin and streptomycin, Pen-Strep, Cat. #15070063, Gibco, Thermo Fisher Scientific, Vantaa, Finland).

Techniques: Positive Control, Control, Incubation, Fluorescence

Effect of C. asiatica EVs on melanin production. Inhibitory effects of different concentrations of C. asiatica EVs on ( A ) melanin content and ( B ) intracellular tyrosinase activity in B16F10 cells. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: In Vitro Characterization of Centella asiatica Extracellular Vesicles and Their Skin Repair Effects in a UVB-Irradiated Mouse Model

doi: 10.3390/ijms26188982

Figure Lengend Snippet: Effect of C. asiatica EVs on melanin production. Inhibitory effects of different concentrations of C. asiatica EVs on ( A ) melanin content and ( B ) intracellular tyrosinase activity in B16F10 cells. Data are presented as mean ± SD ( n = 3). Statistical results were calculated by one-way ANOVA with Dunnett’s multiple comparisons test compared to the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The following cell lines were used in the study: - B16F10 (mouse melanoma, BCRC #60031, ATCC CRL6475) was maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (Penicillin and streptomycin, Pen-Strep, Cat. #15070063, Gibco, Thermo Fisher Scientific, Vantaa, Finland).

Techniques: Activity Assay, Control