60031 Search Results


melanoma b16 f10 cells  (ATCC)
92
ATCC melanoma b16 f10 cells
Melanoma B16 F10 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/melanoma b16 f10 cells/product/ATCC
Average 92 stars, based on 1 article reviews
melanoma b16 f10 cells - by Bioz Stars, 2026-02
92/100 stars
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c 6 nbd sm  (Biotium)
91
Biotium c 6 nbd sm
C 6 Nbd Sm, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c 6 nbd sm/product/Biotium
Average 91 stars, based on 1 article reviews
c 6 nbd sm - by Bioz Stars, 2026-02
91/100 stars
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pde3b  (BPS Bioscience)
93
BPS Bioscience pde3b
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Pde3b, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
pde3b - by Bioz Stars, 2026-02
93/100 stars
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glass microelectrodes filled with ion-selective cocktail k+ 60031  (Fluka Chemical)
90
Fluka Chemical glass microelectrodes filled with ion-selective cocktail k+ 60031
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Glass Microelectrodes Filled With Ion Selective Cocktail K+ 60031, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glass microelectrodes filled with ion-selective cocktail k+ 60031/product/Fluka Chemical
Average 90 stars, based on 1 article reviews
glass microelectrodes filled with ion-selective cocktail k+ 60031 - by Bioz Stars, 2026-02
90/100 stars
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potassium ion exchanger resin fluka 60031 potassium ionophore i - cocktail a  (Fluka Chemical)
90
Fluka Chemical potassium ion exchanger resin fluka 60031 potassium ionophore i - cocktail a
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Potassium Ion Exchanger Resin Fluka 60031 Potassium Ionophore I Cocktail A, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/potassium ion exchanger resin fluka 60031 potassium ionophore i - cocktail a/product/Fluka Chemical
Average 90 stars, based on 1 article reviews
potassium ion exchanger resin fluka 60031 potassium ionophore i - cocktail a - by Bioz Stars, 2026-02
90/100 stars
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ly-6 g stemcell cat# 60031 antibody  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc ly-6 g stemcell cat# 60031 antibody
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Ly 6 G Stemcell Cat# 60031 Antibody, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ly-6 g stemcell cat# 60031 antibody/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
ly-6 g stemcell cat# 60031 antibody - by Bioz Stars, 2026-02
90/100 stars
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ionophore fluka 60031  (Fluka Chemical)
90
Fluka Chemical ionophore fluka 60031
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Ionophore Fluka 60031, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionophore fluka 60031/product/Fluka Chemical
Average 90 stars, based on 1 article reviews
ionophore fluka 60031 - by Bioz Stars, 2026-02
90/100 stars
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fluka 60031 ionophore  (Fluka Chemie)
90
Fluka Chemie fluka 60031 ionophore
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Fluka 60031 Ionophore, supplied by Fluka Chemie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluka 60031 ionophore/product/Fluka Chemie
Average 90 stars, based on 1 article reviews
fluka 60031 ionophore - by Bioz Stars, 2026-02
90/100 stars
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r-60031 silicone-alkyd copolymer  (Dow Corning)
90
Dow Corning r-60031 silicone-alkyd copolymer
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
R 60031 Silicone Alkyd Copolymer, supplied by Dow Corning, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r-60031 silicone-alkyd copolymer/product/Dow Corning
Average 90 stars, based on 1 article reviews
r-60031 silicone-alkyd copolymer - by Bioz Stars, 2026-02
90/100 stars
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valinomycin-based cocktail fluka 60031  (Fluka Chemical)
90
Fluka Chemical valinomycin-based cocktail fluka 60031
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Valinomycin Based Cocktail Fluka 60031, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/valinomycin-based cocktail fluka 60031/product/Fluka Chemical
Average 90 stars, based on 1 article reviews
valinomycin-based cocktail fluka 60031 - by Bioz Stars, 2026-02
90/100 stars
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valinomycinbased k1 ion neutral carrier potassium ionophore i –cocktail a fluka 60031  (Fluka Chemical)
90
Fluka Chemical valinomycinbased k1 ion neutral carrier potassium ionophore i –cocktail a fluka 60031
a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
Valinomycinbased K1 Ion Neutral Carrier Potassium Ionophore I –Cocktail A Fluka 60031, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by <t>PDE3B</t> incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.
K Ionophore Resin Fluka 60031, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k ionophore resin fluka 60031/product/Fluka Chemical
Average 90 stars, based on 1 article reviews
k ionophore resin fluka 60031 - by Bioz Stars, 2026-02
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a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by PDE3B incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Adipocyte lysoplasmalogenase TMEM86A regulates plasmalogen homeostasis and protein kinase A-dependent energy metabolism

doi: 10.1038/s41467-022-31805-3

Figure Lengend Snippet: a , b Immunoblot analysis of C3H10T1/2 adipocytes treated with vehicle control (VC), LPC P-18:0 (LPC-P, 10 μM) or LPE P-18:0 (LPE-P, 10 μM) for 24 h or isoproterenol (ISO, 10 μM) for 4 h. n = 4. c , d Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, ISO (10 μM), LPC-P (10 μM), LPE-P (10 μM), ISO (10 μM) + H89 (PKA inhibitor, 50 μM), LPC-P (10 μM) + H89 (50 μM), or LPE-P (10 μM)+H89 (50 μM). n = 3. (p-HSL/HSL VC vs. ISO: p < 0.000001; VC vs. LPC-P: p = 0.000128; VC vs. LPE-P: p = 0.000009; ISO vs. ISO + H89: p < 0.000001; LPC-P vs. LPC-P + H89: p = 0.000056; LPE-P vs. LPE-P + H89: p = 0.000006)(p-CREB/CREB VC vs. ISO: p = 0.000035; VC vs. LPC-P: p = 0.000018; VC vs. LPE-P: p = 0.000019; ISO vs. ISO + H89: p = 0.000172; LPC-P vs. LPC-P + H89: p = 0.000143; LPE-P vs. LPE-P + H89: p = 0.000109). e Intracellular cAMP levels in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM), or LPE-P (10 μM) for 24 h or ISO (10 μM) for 4 h. n = 3. f Levels of 5' AMP released by PDE3B incubated with VC, IBMX (40 μM), or LPE-P (10 μM) for 90 min. n = 3. g , h Immunoblot analysis of C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 48 h or isoproterenol (ISO, 10 μM) for 24 h. n = 4. i , j Oxygen consumption rate measurement in C3H10T1/2 adipocytes treated with VC, LPC-P (10 μM) or LPE-P (10 μM) for 24 h. n = 5. Each point represents a biological replicate. Data are presented as mean ± SEM. Statistical significance was determined by the unpaired, two-tailed t -test in b , d , e , f , h and j . * represents statistical analysis between C3H10T1/2 adipocytes treated with VC and C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P. # represents statistical analysis between C3H10T1/2 adipocytes treated with either ISO, LPC-P, or LPE-P and C3H10T1/2 adipocytes treated with either ISO + H89, LPC-P + H89, or LPE-P + H89. Source data are provided as a Source Data file.

Article Snippet: Briefly, the mixtures of cAMP substrate (200 nM), assay buffer, 5'-nucleotidase (1 kU/μL ) , and PDE3B (40 pg/reaction, BPS Bioscience, cat#60031) with LPE P-18:0 (10 μM) or IBMX (40 μM) were incubated in a microtiter plate for 0, 45, or 90 min at 30 °C.

Techniques: Western Blot, Incubation, Two Tailed Test