human bladder epithelial 5637 cells  (ATCC)

Journal: iScience

Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

doi: 10.1016/j.isci.2026.116333

Figure Lengend Snippet: rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .

Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

Techniques: Infection, Cell Culture, Confocal Microscopy, Two Tailed Test, MANN-WHITNEY

Journal: iScience

Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

doi: 10.1016/j.isci.2026.116333

Figure Lengend Snippet: RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .

Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

Techniques: Expressing, Cell Culture, Infection, Control, Two Tailed Test