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5637 cells  (ATCC)


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    ATCC 5637 cells
    rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in <t>5637</t> cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of <t>WT</t> <t>cultured</t> in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .
    5637 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 19481 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5637/RPMI-1640+Medium/pmc13276309-364-0-2
    Average 99 stars, based on 19481 article reviews
    5637 cells - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion"

    Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

    Journal: iScience

    doi: 10.1016/j.isci.2026.116333

    rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .
    Figure Legend Snippet: rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .

    Techniques Used: Infection, Cell Culture, Confocal Microscopy, Two Tailed Test, MANN-WHITNEY

    RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .
    Figure Legend Snippet: RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .

    Techniques Used: Expressing, Cell Culture, Infection, Control, Two Tailed Test

    Related Articles

    Cell Culture:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi

    Modification:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi

    Sequencing:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi

    Recombinant:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi

    Infection:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi

    Bacteria:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi

    Stable Transfection:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi

    RNA sequencing:

    Article Title: Inhibition of Cyclin-dependent Kinase Counteracts Neuron-promoted Bladder and Endometrial Tumorigenesis: A Systematic Analysis
    Article Snippet: ) and the endometrial cancer cell line RL95-2 were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan, ROC). The human neuroblastoma cell line SH-SY5Y was obtained from the American Type Culture Collection (Manassas, VA, USA). HTB-9 TM cells were cultured in RPMI medium (Procell, Houston, TX, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium/F12 medi



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    rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in <t>5637</t> cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of <t>WT</t> <t>cultured</t> in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .
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    rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .

    Journal: iScience

    Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

    doi: 10.1016/j.isci.2026.116333

    Figure Lengend Snippet: rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .

    Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

    Techniques: Infection, Cell Culture, Confocal Microscopy, Two Tailed Test, MANN-WHITNEY

    RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .

    Journal: iScience

    Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

    doi: 10.1016/j.isci.2026.116333

    Figure Lengend Snippet: RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .

    Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

    Techniques: Expressing, Cell Culture, Infection, Control, Two Tailed Test