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fibcon proteins  (Sino Biological)


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    Structured Review

    Sino Biological fibcon proteins
    Fibcon Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/50737/Mouse+SELP+selectin+P+P-selectin+Protein/10__1038_slash_s41467___026___78095___7-336-11-19
    Average 93 stars, based on 5 article reviews
    fibcon proteins - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    SPR Assay:

    Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation
    Article Snippet: .. For SPR binding assays, binding affinity (KD) and kinetic parameters ((Ka and Kd) were determined using a recombinant mouse P-selectin ligand (SinoBiological) printed onto a bare gold-coated (47 nm) PlexArray Nanocapture Sensor Chip (Plexera Bioscience). .. Analyses were performed by Creative Peptides (Shirley, NY) with real-time binding signals recorded and analyzed by Data Analysis Module (DAM, Plexera, Bioscience, Seattle, WA), and kinetic analysis performed using BIAevaluation 4.1 software (Biacore, Inc.).

    Binding Assay:

    Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation
    Article Snippet: .. For SPR binding assays, binding affinity (KD) and kinetic parameters ((Ka and Kd) were determined using a recombinant mouse P-selectin ligand (SinoBiological) printed onto a bare gold-coated (47 nm) PlexArray Nanocapture Sensor Chip (Plexera Bioscience). .. Analyses were performed by Creative Peptides (Shirley, NY) with real-time binding signals recorded and analyzed by Data Analysis Module (DAM, Plexera, Bioscience, Seattle, WA), and kinetic analysis performed using BIAevaluation 4.1 software (Biacore, Inc.).

    Recombinant:

    Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation
    Article Snippet: .. For SPR binding assays, binding affinity (KD) and kinetic parameters ((Ka and Kd) were determined using a recombinant mouse P-selectin ligand (SinoBiological) printed onto a bare gold-coated (47 nm) PlexArray Nanocapture Sensor Chip (Plexera Bioscience). .. Analyses were performed by Creative Peptides (Shirley, NY) with real-time binding signals recorded and analyzed by Data Analysis Module (DAM, Plexera, Bioscience, Seattle, WA), and kinetic analysis performed using BIAevaluation 4.1 software (Biacore, Inc.).

    Article Title: A pyroptosis-enhanced leucocyte-hitchhiking liposomal nanoplatform for potentiated immunotherapy of hepatocellular carcinoma
    Article Snippet: Hepatocellular carcinoma (HCC) is a highly malignant tumor with unsatisfactory response to immunotherapy.. Pyroptosis, a recently discovered form of regulated cell death (RCD), possesses a huge potential to enhance the immunotherapy efficiency against HCC.. To achieve efficient drug delivery and ideal activation of antitumor immunity, an E-selectin modified liposomal nanoplatform co-loading gemcitabine elaidate and BMS-202 (a small molecule programmed cell death protein 1 (PD-1)/programmed death ligand 1 (PD-L1) inhibitor) was designed.

    Article Title: Real-time cancer monitoring via leukocyte adhesion in a biomimetic microfluidic assay.
    Article Snippet: Cancer progression involves cell adhesion molecules (CAMs), which facilitate the recruitment of leukocytes and metastatic tumor cells to distant organs by mediating adhesion with endothelial cells.. While CAM-mediated tumor dissemination is well studied, the diagnostic potential of CAM ligand-expressing activated leukocytes as biomarkers reflecting the host’s inflammatory response to cancer remains relatively unexplored.. This study presents a microfluidic device that assesses cancer-driven leukocyte activation in a 4T1 breast cancer mouse model by quantifying leukocyte adhesion to CAM-coated microchannels under physiological flow conditions.

    other:

    Article Title: NIR-II scattering gold superclusters for intravascular optical coherence tomography molecular imaging.
    Article Snippet: Currently, intravascular optical coherence tomography (IV-OCT) is limited to anatomical imaging, providing structural information about atherosclerotic plaque morphology, thrombus and dissection.. Earlier detection and risk stratification would be possible through molecular characterization of endothelium but necessitates a purpose-engineered IV-OCT contrast agent.. Here we developed gold superclusters (AuSCs) tailored to clinical instrumentation and integrated into clinically relevant workflows.

    Sterility:

    Article Title: Real-time cancer monitoring via leukocyte adhesion in a biomimetic microfluidic assay.
    Article Snippet: Cancer progression involves cell adhesion molecules (CAMs), which facilitate the recruitment of leukocytes and metastatic tumor cells to distant organs by mediating adhesion with endothelial cells.. While CAM-mediated tumor dissemination is well studied, the diagnostic potential of CAM ligand-expressing activated leukocytes as biomarkers reflecting the host’s inflammatory response to cancer remains relatively unexplored.. This study presents a microfluidic device that assesses cancer-driven leukocyte activation in a 4T1 breast cancer mouse model by quantifying leukocyte adhesion to CAM-coated microchannels under physiological flow conditions.

    Protein Concentration:

    Article Title: Tall adhesions overcome cell surface steric interference and rescue antibody-dependent phagocytosis in macrophages
    Article Snippet: .. For experiments in which P-selectin was displayed on target surfaces alongside Fibcon proteins and anti-biotin IgG, His-tagged mouse P-selectin (Sino Biological, 50737-M08H) and Fibcon were added at different ratios, while keeping the total protein concentration fixed at 50nM. .. Measuring protein densities on target particles Fib3L proteins were labeled with Alexa Fluor 647 at a single C-terminal cysteine labeling site using Alexa Fluor 647 C2 Maleimide (Thermo Fisher Scientific, Invitrogen, A20347).



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    Image Search Results


    DUB-IN-3 inhibits ATC progression by activating ferroptosis. (A-C) The CCK8 assay (A), colony formation assay (B), and transwell assay (C) was applied to present the effect of DUB-IN-3 on the proliferation, colony formation, and migration capability of BHT101 cells. (D) The CCK8 assay was used to assess the responses of BHT101 cells with DUB-IN-3 treatment to RSL-3 ± Fer-1. (E, F) Lipid ROS (E) and GSH (F) levels in DUB-IN-3 treating ATC cells. (G) The parental BHT101 cells were injected into the right underarm of nude mouse, then DUB-IN-3 was intraperitoneally injected every 3 days ( n = 3 mouse per group). The tumors were removed and weighted after 17 days. (H) Representative images of IHC staining for Ki-67. (I) GPR34 stabilized by deubiquitinase USP8 promotes ATC progression via inhibiting ferroptosis. Ns, nonsignificant. p > 0.05, ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, ⁣ ∗∗∗ p < 0.001.

    Journal: Mediators of Inflammation

    Article Title: GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC

    doi: 10.1155/mi/5576056

    Figure Lengend Snippet: DUB-IN-3 inhibits ATC progression by activating ferroptosis. (A-C) The CCK8 assay (A), colony formation assay (B), and transwell assay (C) was applied to present the effect of DUB-IN-3 on the proliferation, colony formation, and migration capability of BHT101 cells. (D) The CCK8 assay was used to assess the responses of BHT101 cells with DUB-IN-3 treatment to RSL-3 ± Fer-1. (E, F) Lipid ROS (E) and GSH (F) levels in DUB-IN-3 treating ATC cells. (G) The parental BHT101 cells were injected into the right underarm of nude mouse, then DUB-IN-3 was intraperitoneally injected every 3 days ( n = 3 mouse per group). The tumors were removed and weighted after 17 days. (H) Representative images of IHC staining for Ki-67. (I) GPR34 stabilized by deubiquitinase USP8 promotes ATC progression via inhibiting ferroptosis. Ns, nonsignificant. p > 0.05, ⁣ ∗ p < 0.05, ⁣ ∗∗ p < 0.01, ⁣ ∗∗∗ p < 0.001.

    Article Snippet: DUB-IN-3 (HY-50737-11866) were obtained from MedChemExpress.

    Techniques: CCK-8 Assay, Colony Assay, Transwell Assay, Migration, Injection, Immunohistochemistry