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Sino Biological
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation
doi: 10.3389/fimmu.2021.785229
Figure Lengend Snippet: B.PSel-Crry and NB.PSel-Crry reduce inflammation markers after hindlimb ischemia and reperfusion. A murine hindlimb IRI model was used to evaluate the effect of B.PSel and NB.PSel administered i.v. upon reperfusion after 2 h ischemia. (A) Representative images of sections stained for MPO. At the higher dose of 0.5 mg, there were lower numbers of infiltrating myeloid cells in mice treated with B.PSel-Crry compared to NB.PSel-Crry. Scale bars, 50μm. (B) Quantification of infiltrating myeloid cells performed by assessing the number of MPO-positive cells per field. Parametric One-way ANOVA with Tukey’s correction. (C) Representative images of sections stained for P-selectin. Scale bars, 50μm. (D) Quantification of P-selectin expression, which was significantly lower in B.PSel-Crry treated mice compared to NB.PSel-Crry treated mice at both concentrations. Parametric One-Way ANOVA with Tukey’s correction. Quantification data expressed as Mean ± SD, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. n = 5 for all groups.
Article Snippet: For SPR binding assays, binding affinity (KD) and kinetic parameters ((Ka and Kd) were determined using a
Techniques: Staining, Expressing