Journal: bioRxiv

Article Title: Sialidase variability in Gardnerella : genetics, taxonomy, function, and clinical presentation

doi: 10.64898/2026.01.10.698787

Figure Lengend Snippet:

Article Snippet: The type strain of G. vaginalis (DSM 4944) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) and -80 °C freezer stocks were prepared as described above.

Techniques:

Journal: Plant Biotechnology Journal

Article Title: MsWRKY49 Modulates Pollen Tube Elongation in Response to Boron Deficiency by Regulating Reactive Oxygen Species Homeostasis in Alfalfa

doi: 10.1111/pbi.70366

Figure Lengend Snippet: Low boron inhibits pollen tube elongation by increasing ROS levels in alfalfa. (a) Fluorescence microscopy of pollen tubes stained with the ROS sensor CM‐H 2 DCFDA under low boron conditions. BF refers to bright field. Scale bars = 10 μm. (b) Quantification of DCF fluorescence intensity in pollen tubes. Statistical significance is denoted by **, corresponding to p < 0.01. (c) DCF fluorescence intensity along the length of the pollen tube under low boron conditions. (d) Fluorescence microscopy of pollen tubes stained with the H 2 O 2 ‐specific dye PO1 under low boron conditions. BF refers to bright field. Scale bars = 10 μm. (e) Quantification of PO1 fluorescence intensity in pollen tubes. Mean ± SE values were determined from three biological replicates. n = 20–25. Statistical significance is denoted by **, corresponding to p < 0.01. (f) PO1 fluorescence intensity along the length of the pollen tube under low boron conditions. n = 15, and the shaded area represents the SE value from three independent experiments. (g) The effect of 1 mM H 2 O 2 treatment on pollen tube elongation under low boron conditions. Scale bars = 500 μm. (h) Statistical analysis of pollen tube length after 1 mM H 2 O 2 treatment. n = 300, mean ± SD from three independent experiments. Different letters represent significant differences at p < 0.05 (one‐way ANOVA). (i) Effect of 40 μM AsA treatment on pollen tube elongation under low boron conditions. Scale bars = 500 μm. (j) Statistical analysis of pollen tube length after 40 μM AsA treatment. n = 200, mean ± SD values were determined from three independent experiments. Different letters represent significant differences at p < 0.05 (one‐way ANOVA).

Article Snippet: Subsequently, the pollen tubes were stained with 10 μM 5‐(and 6)‐chloromethyl‐2′,7′‐dichlorodihydrofluorescein diacetate (CM‐H 2 DCFDA) (Thermo Fisher Scientific, USA) or 5 μM Peroxy Orange 1 (PO1) (Tocris Bioscience, UK) for 10 min at room temperature in the dark.

Techniques: Fluorescence, Microscopy, Staining

Journal: Plant Biotechnology Journal

Article Title: MsWRKY49 Modulates Pollen Tube Elongation in Response to Boron Deficiency by Regulating Reactive Oxygen Species Homeostasis in Alfalfa

doi: 10.1111/pbi.70366

Figure Lengend Snippet: The mswrky49 mutant promotes pollen tube elongation under low‐boron conditions by reducing ROS accumulation. (a) Pollen tube phenotypes of WT and mswrky49 mutants under different boron concentrations. Scale bars = 500 μm. (b) Length measurements of WT and mswrky49 mutant pollen tubes after 1 h of different boron concentrations treatment. The data represent mean ± SD of three independent experiments ( n = 200). Different letters represent significant differences at p < 0.05 (one‐way ANOVA). (c) Observation of H 2 O 2 levels in WT and mswrky49 mutant pollen tubes under different boron concentrations using PO1 fluorescence staining. Scale bars = 10 μm. (d) Quantification of fluorescence intensity of PO1 in WT and mswrky49 mutant pollen tubes under different boron concentrations. The data represent mean ± SE of three independent experiments ( n = 25–20). Different letters represent significant differences at p < 0.05 (one‐way ANOVA).

Article Snippet: Subsequently, the pollen tubes were stained with 10 μM 5‐(and 6)‐chloromethyl‐2′,7′‐dichlorodihydrofluorescein diacetate (CM‐H 2 DCFDA) (Thermo Fisher Scientific, USA) or 5 μM Peroxy Orange 1 (PO1) (Tocris Bioscience, UK) for 10 min at room temperature in the dark.

Techniques: Mutagenesis, Fluorescence, Staining

Journal: Plant Biotechnology Journal

Article Title: MsWRKY49 Modulates Pollen Tube Elongation in Response to Boron Deficiency by Regulating Reactive Oxygen Species Homeostasis in Alfalfa

doi: 10.1111/pbi.70366

Figure Lengend Snippet: Overexpression of MsWRKY49 in alfalfa reduces length of pollen tubes by increasing ROS accumulation. (a) Pollen tube phenotypes of WT and MsWRKY49‐OE under different boron concentrations. Scale bars = 500 μm. (b) Length measurements of WT and MsWRKY49‐OE pollen tubes after 1 h of different boron concentrations treatment. The data represent mean ± SD of three independent experiments ( n = 200). Different letters represent significant differences at p < 0.05 (one‐way ANOVA). (c) Observation of H 2 O 2 levels in WT and MsWRKY49‐OE pollen tubes under different boron concentrations using PO1 fluorescence staining. Scale bars = 10 μm. (d) Quantification of fluorescence intensity of PO1 in WT and MsWRKY49‐OE pollen tubes under different boron concentrations. The data represent mean ± SE of three independent experiments ( n = 15–20). Different letters represent significant differences at p < 0.05 (one‐way ANOVA).

Article Snippet: Subsequently, the pollen tubes were stained with 10 μM 5‐(and 6)‐chloromethyl‐2′,7′‐dichlorodihydrofluorescein diacetate (CM‐H 2 DCFDA) (Thermo Fisher Scientific, USA) or 5 μM Peroxy Orange 1 (PO1) (Tocris Bioscience, UK) for 10 min at room temperature in the dark.

Techniques: Over Expression, Fluorescence, Staining