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e coli  (ATCC)


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    Structured Review

    ATCC e coli
    E Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/49426/Sulfolobus+acidocaldarius+Brock+et+al/pm41179137-169-48-57
    Average 93 stars, based on 43 article reviews
    e coli - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Pseudomonas mendocina Isolated from Anopheles Midguts has a Greater Potential to Build Thick Biofilms than Serratia marcescens .
    Article Snippet: The pooled midgut macerate was added to 5 mL of MHB (BD Difco, Franklin Lakes, New Jersey, USA) and incubated at 37 °C with 180 rpm agitation for 18−20 h. 10-fold serial dilutions of the overnight culture were prepared in PBS, and 100 μL aliquots of the 10−6, 10−7, and 10−8 dilutions were plated on: MHA (BD Difco); PIA (BD Difco); MacConkey II agar (BD BBL); CHROMagar Pseudomonas (CHROMagar, Kanto Chemical Co, Tokyo, Japan); and blood agar base (Sheep Blood 7%) (LiofilChem, Roseto degli Abruzzi (TE), Italy) (three replicates per dilution per medium).

    Article Title: Microorganisms and methods for the production of butadiene using acetyl-coA
    Article Snippet: M2, Pyrococcus abyssi, Pyrococcus furiosus, Pyrococcus horikoshii OT3, Pyrobaculum islandicum DSM 4184, Ralstonia eutropha, Ralstonia eutropha H16, Ralstonia eutropha JMP134, Ralstonia metallidurans, Ralstonia pickettii, Rattus norvegicus, Rhizobium leguminosarum, Rhodobacter capsulatus, Rhodobacter sphaeroides, Rhodobacter sphaeroides ATCC 17025, Rhodococcus ruber, Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009, Rhodospirillum rubrum, Roseiflexus castenholzii, Saccharomyces cerevisae, Saccharomyces cerevisiae S288c, Salinispora arenicola, Salmonella enterica, Salmonella typhimurium, Salmonella typhimurium LT2, Salmonella enterica subsp. enterica serovar Typhimurium str.

    Polymerase Chain Reaction:

    Article Title: Method for constructing trehalose multi-enzyme complex in vitro mediated by artificial scaffold protein
    Article Snippet: Source of Biological Materials: Bacillus subtilis WB800N was purchased from Hangzhou BIO SCI Biotechnology Co., Ltd.; yeast episomal plasmid pHT01 was purchased from UNIBIO Biotechnology Co., Ltd.; Sulfolobus acidocaldarius was deposit in the laboratory with the accession number of ATCC 33909; Sase2-Ccdoc bacterial culture was purchased from Shanghai Sangon Biotech Company; E. coli strain P43-phoD-MTHase is constructed according to conventional techniques. .. In one method, the construction of P43-phoD-MTHase comprises the following steps: designing primers and amplifying a gene fragment of P43 promoter and a gene fragment of phoD signal peptide through PCR, using a gene of Bacillus subtilis WB800n as a template; and the primers are: P43-F (SEQ ID NO: 6): 5′-AGTGAATTCGAGCTCAGCTTCGTGCATGCAGGCCGG-3′; P43-R (SEQ ID NO: 7): 5′-TCAAAACGACTGTCGTATGCCATAAGCTTCTGTTATTA ATTCTTGTCT-3′ phoD-Hase-F (SEQ ID NO: 17): 5′-GAATTAATAACAGAAGCTTATGGCATACGACAGTCGTTT TGATG-3′; phoD-Hase-R (SEQ ID NO: 18): 5′-TGCCCGGAACTTTATACGTTTCTAATTGATATACCCCA ACACCT-3′; amplifying a gene fragment treZ of malto-oligosaccharyl trehalose hydrolase (MTHase) through PCR, using the genome of Sulfolobus acidocaldarius having a accession number of ATCC 33909 as a template, and the primers are: treZ-F (SEQ ID NO: 25): 5′-GCAAATGGGTCGCGGATCCATGTTTTCGTTCGGTGGAAAT-3′ treZ-R (SEQ ID NO: 26): 5′-GTCGACTCTAGATCATTCTAATTGATATAC-3′; ligating these fragments by using a multi-fragment seamless cloning technology and transforming the ligated fragments into E. coli DH5α competent cells, and verifying for successful transformation of the fragments to obtain an E. coli strain P43-phoD-MTHase. .. Hase2-Ctdoc bacterial culture was purchased from Shanghai Sangon Biotech Company; CGTase-rfdoc bacterial culture was purchased from Shanghai Sangon Biotech Company.

    Article Title: Method for constructing trehalose multi-enzyme complex in vitro mediated by artificial scaffold protein
    Article Snippet: .. The gene fragment treY of malto-oligosaccharyl trehalose synthase (MTSase) was subject to PCR amplification PCR amplification, using Sulfolobus acidocaldarius having an accession number of ATCC 33909 genome as a template. .. Primers were designed to amplify the gene fragment Ccdoc of dockerin, using Sase2-Ccdoc bacterial culture synthesized by Shanghai Sangon Biotech Company as a template.

    Cloning:

    Article Title: Method for constructing trehalose multi-enzyme complex in vitro mediated by artificial scaffold protein
    Article Snippet: Source of Biological Materials: Bacillus subtilis WB800N was purchased from Hangzhou BIO SCI Biotechnology Co., Ltd.; yeast episomal plasmid pHT01 was purchased from UNIBIO Biotechnology Co., Ltd.; Sulfolobus acidocaldarius was deposit in the laboratory with the accession number of ATCC 33909; Sase2-Ccdoc bacterial culture was purchased from Shanghai Sangon Biotech Company; E. coli strain P43-phoD-MTHase is constructed according to conventional techniques. .. In one method, the construction of P43-phoD-MTHase comprises the following steps: designing primers and amplifying a gene fragment of P43 promoter and a gene fragment of phoD signal peptide through PCR, using a gene of Bacillus subtilis WB800n as a template; and the primers are: P43-F (SEQ ID NO: 6): 5′-AGTGAATTCGAGCTCAGCTTCGTGCATGCAGGCCGG-3′; P43-R (SEQ ID NO: 7): 5′-TCAAAACGACTGTCGTATGCCATAAGCTTCTGTTATTA ATTCTTGTCT-3′ phoD-Hase-F (SEQ ID NO: 17): 5′-GAATTAATAACAGAAGCTTATGGCATACGACAGTCGTTT TGATG-3′; phoD-Hase-R (SEQ ID NO: 18): 5′-TGCCCGGAACTTTATACGTTTCTAATTGATATACCCCA ACACCT-3′; amplifying a gene fragment treZ of malto-oligosaccharyl trehalose hydrolase (MTHase) through PCR, using the genome of Sulfolobus acidocaldarius having a accession number of ATCC 33909 as a template, and the primers are: treZ-F (SEQ ID NO: 25): 5′-GCAAATGGGTCGCGGATCCATGTTTTCGTTCGGTGGAAAT-3′ treZ-R (SEQ ID NO: 26): 5′-GTCGACTCTAGATCATTCTAATTGATATAC-3′; ligating these fragments by using a multi-fragment seamless cloning technology and transforming the ligated fragments into E. coli DH5α competent cells, and verifying for successful transformation of the fragments to obtain an E. coli strain P43-phoD-MTHase. .. Hase2-Ctdoc bacterial culture was purchased from Shanghai Sangon Biotech Company; CGTase-rfdoc bacterial culture was purchased from Shanghai Sangon Biotech Company.

    Transformation Assay:

    Article Title: Method for constructing trehalose multi-enzyme complex in vitro mediated by artificial scaffold protein
    Article Snippet: Source of Biological Materials: Bacillus subtilis WB800N was purchased from Hangzhou BIO SCI Biotechnology Co., Ltd.; yeast episomal plasmid pHT01 was purchased from UNIBIO Biotechnology Co., Ltd.; Sulfolobus acidocaldarius was deposit in the laboratory with the accession number of ATCC 33909; Sase2-Ccdoc bacterial culture was purchased from Shanghai Sangon Biotech Company; E. coli strain P43-phoD-MTHase is constructed according to conventional techniques. .. In one method, the construction of P43-phoD-MTHase comprises the following steps: designing primers and amplifying a gene fragment of P43 promoter and a gene fragment of phoD signal peptide through PCR, using a gene of Bacillus subtilis WB800n as a template; and the primers are: P43-F (SEQ ID NO: 6): 5′-AGTGAATTCGAGCTCAGCTTCGTGCATGCAGGCCGG-3′; P43-R (SEQ ID NO: 7): 5′-TCAAAACGACTGTCGTATGCCATAAGCTTCTGTTATTA ATTCTTGTCT-3′ phoD-Hase-F (SEQ ID NO: 17): 5′-GAATTAATAACAGAAGCTTATGGCATACGACAGTCGTTT TGATG-3′; phoD-Hase-R (SEQ ID NO: 18): 5′-TGCCCGGAACTTTATACGTTTCTAATTGATATACCCCA ACACCT-3′; amplifying a gene fragment treZ of malto-oligosaccharyl trehalose hydrolase (MTHase) through PCR, using the genome of Sulfolobus acidocaldarius having a accession number of ATCC 33909 as a template, and the primers are: treZ-F (SEQ ID NO: 25): 5′-GCAAATGGGTCGCGGATCCATGTTTTCGTTCGGTGGAAAT-3′ treZ-R (SEQ ID NO: 26): 5′-GTCGACTCTAGATCATTCTAATTGATATAC-3′; ligating these fragments by using a multi-fragment seamless cloning technology and transforming the ligated fragments into E. coli DH5α competent cells, and verifying for successful transformation of the fragments to obtain an E. coli strain P43-phoD-MTHase. .. Hase2-Ctdoc bacterial culture was purchased from Shanghai Sangon Biotech Company; CGTase-rfdoc bacterial culture was purchased from Shanghai Sangon Biotech Company.

    Amplification:

    Article Title: Method for constructing trehalose multi-enzyme complex in vitro mediated by artificial scaffold protein
    Article Snippet: .. The gene fragment treY of malto-oligosaccharyl trehalose synthase (MTSase) was subject to PCR amplification PCR amplification, using Sulfolobus acidocaldarius having an accession number of ATCC 33909 genome as a template. .. Primers were designed to amplify the gene fragment Ccdoc of dockerin, using Sase2-Ccdoc bacterial culture synthesized by Shanghai Sangon Biotech Company as a template.



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