akt activator sc79 (Tocris)

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Tocris akt activator sc79

Fig. 3 dis3l2 is essential for neural crest survival. A: Skeletal preparation and head measurements of dis3l2 depleted larvae showing smaller head area with respect to control. B: Cell death analysis in the dis3l2 morphants by acridine orange staining. Acridine orange positive cells (white asterisk) shown in the head. Scale bars, 200 μm. C: Relative expression of Bcl2 family members– bim, puma, foxo, and bcl2 in dis3l2 morphants. Data are shown as mean + SEM, *p < 0.05, **p < 0.01, ***p < 0.001. n = 3 for each experiment, with a minimum of 100 embryos per experiment. D: Western blot analysis to show Caspase3 levels in morphants embryos. Gapdh was used as loading control. E: Western blot analysis showing phosphorylated Akt with respect to total Akt levels and phosphorylated Gsk3β with respect to total Gsk3β levels in dis3l2 depleted embryos. Gapdh was used as loading control. F: Rescue experiments showing phenotype P1 and P2 upon treatment with Akt activator <t>SC79</t> in dis3l2 morpholino injected embryos. G: Cell death analysis by acridine orange staining in dis3l2 morpholino injected embryos treated with SC79 and WT embryos treated with SC79

Akt Activator Sc79, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4635/SC+79/pm40500755-87-22-25
Average 93 stars, based on 49 article reviews

akt activator sc79 - by Bioz Stars, 2026-11

93/100 stars

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1) Product Images from "Dis3l2 is essential for neural crest survival by modulating Akt signaling."

Article Title: Dis3l2 is essential for neural crest survival by modulating Akt signaling.

Journal: Cell communication and signaling : CCS

doi: 10.1186/s12964-025-02288-8

Figure Legend Snippet: Fig. 3 dis3l2 is essential for neural crest survival. A: Skeletal preparation and head measurements of dis3l2 depleted larvae showing smaller head area with respect to control. B: Cell death analysis in the dis3l2 morphants by acridine orange staining. Acridine orange positive cells (white asterisk) shown in the head. Scale bars, 200 μm. C: Relative expression of Bcl2 family members– bim, puma, foxo, and bcl2 in dis3l2 morphants. Data are shown as mean + SEM, *p < 0.05, **p < 0.01, ***p < 0.001. n = 3 for each experiment, with a minimum of 100 embryos per experiment. D: Western blot analysis to show Caspase3 levels in morphants embryos. Gapdh was used as loading control. E: Western blot analysis showing phosphorylated Akt with respect to total Akt levels and phosphorylated Gsk3β with respect to total Gsk3β levels in dis3l2 depleted embryos. Gapdh was used as loading control. F: Rescue experiments showing phenotype P1 and P2 upon treatment with Akt activator SC79 in dis3l2 morpholino injected embryos. G: Cell death analysis by acridine orange staining in dis3l2 morpholino injected embryos treated with SC79 and WT embryos treated with SC79

Techniques Used: Control, Staining, Expressing, Western Blot, Injection

Synthesized:

Article Title: Novel benzoylurea derivative decreases TRPM7 channel function and inhibits cancer cells migration
Article Snippet: Fetal bovine serum (FBS), Roswell Park Memorial Institute (RPMI) 1640 medium, penicillin, streptomycin, and other necessary substances were from Thermo Fisher Scientific Inc. (USA). .. SUD was synthesized in-house by the Department of Pharmaceutical Chemistry, Hebei Medical University; 2-Amino- 6-chloro- α- cyano-3- (ethoxycarbonyl)- 4 H–1- benzopyran-4-acetic Acid Ethyl Ester (SC79) was acquired from Tocris Biosciences (USA) and used at a final concentration of 4 μg/mL. ..

Concentration Assay:

Transduction:

Article Title: HN1-mediated activation of lipogenesis through Akt-SREBP signaling promotes hepatocellular carcinoma cell proliferation and metastasis.
Article Snippet: LY294002 was purchased from Sigma-Aldrich (St. Louis, MO, USA) and diluted in dimethyl sulfoxide (DMSO; Sigma, Saint Louis, MO, USA). .. SC79 was purchased from Tocris Bioscience (Bristol, United Kingdom) and diluted in DMSO (Sigma, Saint Louis, MO, USA). siRNA transduction HepG2 and SNU449 cells were seeded in 6-well plates. .. After overnight incubation, transfection was conducted with a 100 pmol concentration of siRNA in Opti-MEM (Gibco by Life Technologies, Thermo Fisher Scientific Inc., Waltham, MA, USA) and lipofectamine 3000 (Invitrogen, Thermo Fisher Scientific Inc., Waltham, MA, USA).

Injection:

Article Title: DIS3L2 is essential for neural crest survival by modulating Akt signaling
Article Snippet: Rabbit antibodies: anti-γ tubulin (1:2000) (Thermo#PA5-34815), Mouse antibodies: anti-α tubulin (1:100) (CST#3873). .. DIS3L2 morpholino injected embryos at the shield stage (6hpf) were exposed to 1uM of Akt activator SC79 (Tocris, # 4635). ..

other:

Article Title: Oct4 controls basement membrane development during human embryogenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins 3744270, Rac1-activator Key Organics Cat#9T-1505 b-Glycerophosphate disodium salt hydrate Merck Cat#G9422 Accutase cell detachment solution Merck Cat#SCR005 Aprotinin from bovine lung Merck Cat#A1153 Benzamidine hydrochloride hydrate Merck Cat#B6506 Bovine serum albumin Fraction V (pH 7.0) (BSA) AppliChem Cat#A1391 DAPI Merck Cat#D9542 DBPS without Calcium and Magnesium (PBS) Lonza Cat#BE17-512F Dimethyl sulfoxide (DMSO) Merck Cat#D2438 Dispase (5 U/mL) Stemcell Technologies Cat#07913 DMEM/F-12, HEPES Gibco Cat#11330057 DyLight 594 Phalloidin Cell Signaling Technology (CST) Cat#12877S EHT 1864, Rac1-inhibitor Selleckchem Cat#S7482 Ethylenediaminetetraacetic acid (EDTA) disodium salt solution Merck Cat#E7889 EZ-PCR Mycoplasma Detection Kit Biological Industries Cat#20-700-20 Geltrex LDEV-Free, hESC-Qualified RGF BM Matrix Gibco Cat#A1413302 Glutaraldehyde solution, 10% Electron Microscopy Sciences Cat#16120 HEPES Merck Cat#H3375 Intercept (TBS) Blocking Buffer LI-COR Biosciences Cat#927-60001 Intercept T20 (TBS) Antibody Diluent LI-COR Biosciences Cat#927-65001 Leupeptin trifluoroacetate salt Merck Cat#L2023 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778075 Matrigel GFR BM Matrix, LDEV-free Corning Cat#354230 mTeSR1 Stemcell Technologies Cat#85850 Paraformaldehyde solution, 16%, methanol-free Electron Microscopy Sciences Cat#E15710 Phenylmethanesulfonyl fluoride (PMSF) solution Merck Cat#93482 PLL(20 kDa) grafted with PEG(2 kDa) SuSoS Cat#PLL(20)-g[3.5]- PEG(2) Poly-L-lysine solution, 0.1% Merck Cat#P8920 SC79, pan-Akt activator Tocris Cat#4635 Sodium chloride Merck Cat#1.06404 Sodium fluoride Merck Cat#S7920 Triton X-100 Merck Cat#X-100 Trypsin inhibitor from Glycine max (soybean) Merck Cat#T9003 Versene (EDTA), 0.02% Lonza Cat#BE17-711E Wheat Germ Agglutinin, Alexa Fluor 488 Invitrogen Cat#W11261 Y-27632 dihydrochloride Tocris Cat#1254 Critical commercial assays Qubit RNA Broad Range (BR) Assay Kit Thermo Fisher Scientific/ Invitrogen Cat#Q10211 Qubit RNA IQ Assay Kit Thermo Fisher Scientific/ Invitrogen Cat#Q33222 Rac1 G-LISA Activation Assay (Luminescence Format) Cytoskeleton Cat#BK126 RNeasy Plus Mini Kit Qiagen Cat#74134 RNA ScreenTape Agilent Cat#5067-5576 RNA ScreenTape Ladder Agilent Cat#5067-5578 RNA ScreenTape Sample Buffer Agilent Cat#5067-5577 Deposited data RNA-seq data This paper GEO: GSE255320 (Continued on next page) e2 Developmental Cell 59, 1439–1456.e1–e7, June 3, 2024

Staining:

Article Title: Dis3l2 is essential for neural crest survival by modulating Akt signaling.
Article Snippet: Rabbit antibodies: anti-γ tubulin (1:2000) (Thermo#PA5-34815), Mouse antibodies: anti-α tubulin (1:100) (CST#3873). .. Akt activator assay and acridine orange staining dis3l2 morpholino-injected embryos at the shield stage (6 hpf) were exposed to 1 μm of Akt activator SC79 (Tocris, # 4635). ..

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