Journal: Scientific Reports

Article Title: Genome-based reclassification of Micromonospora veneta Kaewkla et al. 2022 as a later heterotypic synonym of Micromonospora coerulea Jensen 1932 (Approved lists 1980)

doi: 10.1038/s41598-025-13676-y

Figure Lengend Snippet: The length of the 16S rRNA gene sequences of M. coerulea JCM 3175 T and M. veneta DSM 109713 T are 1,436 bp and 1,438 bp respectively. Maximum-likelihood tree based on 16S rRNA gene sequences, showing the phylogenetic positions of strains DSM 109713 T and JCM 3175 T and related members within the genus Micromonospora . Actinoplanes aksuensis TRM 88003 T (OM 112204) was used as an outgroup. Bootstrap values (expressed as percentages of 1000 replicates) above 50% are shown at the branch points. Bar, 0.01 substitutions per nucleotide position.

Article Snippet: At the time of writing, based on Parte the genus Micromonospora includes 130 species with validly published names ( https://lpsn.dsmz.de/genus/micromonospora ), widely distributed in various environments, including peat swamp forests root noduless hot spring soils and deep sea environments .

Techniques:

Journal: Scientific Reports

Article Title: Genome-based reclassification of Micromonospora veneta Kaewkla et al. 2022 as a later heterotypic synonym of Micromonospora coerulea Jensen 1932 (Approved lists 1980)

doi: 10.1038/s41598-025-13676-y

Figure Lengend Snippet: Pangenome analysis of the 16 Micromonospora type strains. (A) A pangenome map depicting the functional distribution of core gene clusters and unique genes in the selected Micromonospora genomes. (B) The accumulative curve showing the number of core gene clusters in relation to the number of genomes included in the pangenome analysis. The blue line represents the change in number of core gene clusters as the number of genomes included in the pan-genome analysis increases. The orange line typically indicates the number of non-core gene clusters (or gene clusters, variable gene clusters) as the number of genomes included changes. (C) UpSet plot illustrating the unique genes as well as the genes shared between the Micromonospora strains.

Article Snippet: At the time of writing, based on Parte the genus Micromonospora includes 130 species with validly published names ( https://lpsn.dsmz.de/genus/micromonospora ), widely distributed in various environments, including peat swamp forests root noduless hot spring soils and deep sea environments .

Techniques: Functional Assay

Journal: iScience

Article Title: Inhibition of cathepsin L ameliorates inflammation through the A20/NF-κB pathway in endotoxin-induced acute lung injury.

doi: 10.1016/j.isci.2024.111024

Figure Lengend Snippet: Figure 7. CTSL regulates the LPS-induced K63-linked polyUb binding of NEMO by degrading A20 (A) BMDM cells were treated with IAAP or SID26681509 for 6 h, then stimulated with LPS (100 ng/mL) for 5 min, the levels of p-IKKa/b, IkBa, p-IkBa, p65, p-p65, and ACTB were examined using Western blotting. (B) BMDM cells were treated with IAAP or SID26681509 for 6 h, then stimulated with LPS (100 ng/mL) for 5 min. BMDM was analyzed for the spatial approximation of IKKg with K63-linked polyUb components using PLA. Red, proximity ligation-positive signals. Scale bars: 5 mm. The quantification shown on the right represents the fluorescence, n = 15. (C) BMDM cells were treated with IAAP or SID26681509 for 6 h, then stimulated with LPS (100 ng/mL) for 5 min, the levels of A20, NEMO, IKKb, and ACTB were examined using Western blotting. (D) BMDM cells were analyzed for the spatial approximation of A20 with CTSL components using PLA. Red, proximity ligation-positive signals. Scale bars: 5 mm. Quantification shown on the right represents the fluorescence, n = 15. (E) BMDM cells were transfected with control-, A20-siRNA for 24 h. Then cells were treated with IAAP (5 mM) or SID26681509 (5 mM) for 6 h, and then stimulated with LPS (100 ng/mL) for 5 min. Cells were then harvested for analyzing the mRNA expression of IL-6 and CXCL1 using qRT-PCR, n = 3 in each group. DMSO was used as the vehicle control. All the quantitative data are presented as mean G SEM and differences were identified using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001; ns, p > 0.05.

Article Snippet: CTSB (human) Santa cruz Cat#sc-36462 CTSL (human) Santa cruz Catsc-44327 A20 (mouse) GenePharma Cat#A01001 Primers for recombinant DNA This paper see Table S3

Techniques: Binding Assay, Western Blot, Ligation, Transfection, Control, Expressing, Quantitative RT-PCR