Journal: Nature medicine

Article Title: Augmented endothelial exocytosis of angiopoietin-2 resulting from CCM3-deficiency contributes to the progression of cerebral cavernous malformation

doi: 10.1038/nm.4169

Figure Lengend Snippet:

Article Snippet: CCM3, mouse , Santa Cruz , SC365587 , 1:1000.

Techniques: Immunostaining

Journal: Cell Death & Disease

Article Title: SREBP1 site 1 protease inhibitor PF-429242 suppresses renal cell carcinoma cell growth

doi: 10.1038/s41419-021-03999-9

Figure Lengend Snippet: Primary RCC cells (“RCC1/RCC2/RCC3”) or A498 cells, bearing the lentiviral S1P expression construct (“OE-S1P”) or empty vector (“Vec”), were established, expression of listed genes in these cells and parental control cells (“Pare”) was shown ( A – C and G ). Cells were further cultured for applied time periods, CCK-8 OD ( H ), proliferation (by recording nuclear EdU ratio, D and I ), migration, and invasion (“Transwell” assay, E , F , and J ) were tested. RCC1 cells bearing SREBP1 shRNA (“sh-SREBP1”) or SREBP1-expressing construct (OE-SREBP1) were established, control cells were transduced with scrambled control shRNA plus empty vector (“Vec+shC”), expression of SREBP1 mRNA and protein was shown ( K ). Cells were further cultured for applied time periods, viable cell number ( L ), proliferation ( M ), and migration ( N ) were tested similarly. For each assay, n = 5. Data were expressed as the mean ± standard deviation (SD). * P < 0.05 vs. “Vec”/“Vec+shC” group. In this figure, experiments were repeated three times, and similar results were obtained each time.

Article Snippet: SREBP1 shRNA lentiviral particles (sc-44327-V, Santa Cruz Biotech, Santa Cruz, CA) or S1P shRNA lentiviral particles (sc-36496-V, Santa Cruz Biotech) were added to RCC cells for 48 h. Puromycin was added to the medium.

Techniques: Expressing, Construct, Plasmid Preparation, Control, Cell Culture, CCK-8 Assay, Migration, Transwell Assay, shRNA, Transduction, Standard Deviation

Journal: Cell Death & Disease

Article Title: SREBP1 site 1 protease inhibitor PF-429242 suppresses renal cell carcinoma cell growth

doi: 10.1038/s41419-021-03999-9

Figure Lengend Snippet: RCC1 xenograft-bearing SCID mice were subjected to i.v . injection of PF-429242 (“PF”, at 10 mg/kg body weight, daily, for 21days) or vehicle control (“Veh”), tumor volumes ( A ) and mice body weights ( D ) were recorded every seven days; The estimated daily tumor growth, in mm 3 per day, was calculated using the described formula ( B ). On Day-35 tumors of the two groups were separated through surgery and individually weighted ( C ). On Day-7 and Day-14, one tumor of each group was isolated and homogenized in tissue lysis buffer, expression of listed genes was shown ( E and F ). RCC1 xenograft-bearing SCID mice were subjected to intratumoral injection of SREBP1 shRNA lentivirus (“shSREBP1”), S1P shRNA lentivirus (“shS1P”) or scramble control shRNA lentivirus (“shC”), daily for five days, tumor volumes ( G ) and mice body weights ( H ) were recorded every seven days; At Day-14, one tumor of each group was isolated and homogenized in tissue lysis buffer, expression of listed proteins was shown ( I ). Data were expressed as the mean ± standard deviation (SD).* P < 0.05 vs. “Veh”/ “shC” group.

Article Snippet: SREBP1 shRNA lentiviral particles (sc-44327-V, Santa Cruz Biotech, Santa Cruz, CA) or S1P shRNA lentiviral particles (sc-36496-V, Santa Cruz Biotech) were added to RCC cells for 48 h. Puromycin was added to the medium.

Techniques: Injection, Control, Isolation, Lysis, Expressing, shRNA, Standard Deviation

Journal: Cell Death & Disease

Article Title: SREBP1 site 1 protease inhibitor PF-429242 suppresses renal cell carcinoma cell growth

doi: 10.1038/s41419-021-03999-9

Figure Lengend Snippet: Eight ( n = 8) pairs of human RCC tumor tissues (“T”) and surrounding normal renal tissues (“N”) were obtained, expression of listed genes was tested by RT-qPCR ( A - C ) and Western blotting ( D , E ) analyses, results were normalized and quantified. The TCGA cohort shows relative SREBP1 transcripts in 533 cases of ccRCC tissues (“Primary Tumor”) and 72 cases of normal renal tissues (“Normal”) ( F ). Kaplan–Meier Survival analyses of SREBP1 -low ( n = 398, in blue) and SREBP1 -high ( n = 133, in red) ccRCC patients were shown ( G ). Data were expressed as the mean ± standard deviation (SD). * P < 0.05 vs. “N” tissues.

Article Snippet: SREBP1 shRNA lentiviral particles (sc-44327-V, Santa Cruz Biotech, Santa Cruz, CA) or S1P shRNA lentiviral particles (sc-36496-V, Santa Cruz Biotech) were added to RCC cells for 48 h. Puromycin was added to the medium.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation