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( A ) Outline of metabolic inhibitors for screening. ( B ) Human hTERT-immortalized foreskin fibroblast <t>(BJ-5ta,</t> left) and mouse fibroblast (L929, right) cells were treated with inhibitors from A followed by 0.5 μg/mL HT-DNA transfection for 5 hours. Cells were harvested for qPCR analysis of human IFNB or mouse Cxcl10 . ( C ) BJ-5ta cells were treated with 1 mM AOA for 1 hour followed by HT-DNA (0.5 μg/mL) transfection for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( D and E ) L929 ( D ) and MC38 ( E ) were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) or HT-DNA (0.5 μg/mL) stimulation for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( F – H ) Western blot detected phosphorylated (p-) p-IRF3 and p-STAT1 levels in BJ-5ta, L929, and MC38 cells treated as in C – E . ( I ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection or not. Scale bars, 5 μm. ( J ) Schematic of the main targets of AOA. ( K ) Western blot detected p-IRF3, p-STAT1, GOT2, and GPT2 levels in BJ-5ta cells treated as indicated. ( L ) The relative IFNB and CXCL10 mRNA expression in the control BJ-5ta cells versus GOT2 and GPT2 siRNA-silenced BJ-5ta cells after indicated treatment. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by unpaired t test ( C – E , I , and L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Bj 5ta, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4001/BJ-5ta/pmc13221234-237-29-36
Average 96 stars, based on 473 article reviews

bj 5ta - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity"

Article Title: Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity

Journal: The Journal of Clinical Investigation

doi: 10.1172/JCI199716

( A ) Outline of metabolic inhibitors for screening. ( B ) Human hTERT-immortalized foreskin fibroblast (BJ-5ta, left) and mouse fibroblast (L929, right) cells were treated with inhibitors from A followed by 0.5 μg/mL HT-DNA transfection for 5 hours. Cells were harvested for qPCR analysis of human IFNB or mouse Cxcl10 . ( C ) BJ-5ta cells were treated with 1 mM AOA for 1 hour followed by HT-DNA (0.5 μg/mL) transfection for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( D and E ) L929 ( D ) and MC38 ( E ) were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) or HT-DNA (0.5 μg/mL) stimulation for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( F – H ) Western blot detected phosphorylated (p-) p-IRF3 and p-STAT1 levels in BJ-5ta, L929, and MC38 cells treated as in C – E . ( I ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection or not. Scale bars, 5 μm. ( J ) Schematic of the main targets of AOA. ( K ) Western blot detected p-IRF3, p-STAT1, GOT2, and GPT2 levels in BJ-5ta cells treated as indicated. ( L ) The relative IFNB and CXCL10 mRNA expression in the control BJ-5ta cells versus GOT2 and GPT2 siRNA-silenced BJ-5ta cells after indicated treatment. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by unpaired t test ( C – E , I , and L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Figure Legend Snippet: ( A ) Outline of metabolic inhibitors for screening. ( B ) Human hTERT-immortalized foreskin fibroblast (BJ-5ta, left) and mouse fibroblast (L929, right) cells were treated with inhibitors from A followed by 0.5 μg/mL HT-DNA transfection for 5 hours. Cells were harvested for qPCR analysis of human IFNB or mouse Cxcl10 . ( C ) BJ-5ta cells were treated with 1 mM AOA for 1 hour followed by HT-DNA (0.5 μg/mL) transfection for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( D and E ) L929 ( D ) and MC38 ( E ) were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) or HT-DNA (0.5 μg/mL) stimulation for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( F – H ) Western blot detected phosphorylated (p-) p-IRF3 and p-STAT1 levels in BJ-5ta, L929, and MC38 cells treated as in C – E . ( I ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection or not. Scale bars, 5 μm. ( J ) Schematic of the main targets of AOA. ( K ) Western blot detected p-IRF3, p-STAT1, GOT2, and GPT2 levels in BJ-5ta cells treated as indicated. ( L ) The relative IFNB and CXCL10 mRNA expression in the control BJ-5ta cells versus GOT2 and GPT2 siRNA-silenced BJ-5ta cells after indicated treatment. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by unpaired t test ( C – E , I , and L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Techniques Used: Transfection, Gene Expression, Western Blot, Immunofluorescence, Expressing, Control

( A ) Heatmap of metabolite changes in mock- and AOA-treated L929 cells followed with HT-DNA transfection or without HT-DNA transfection. The fold-change of metabolites abundances was normalized to the mock group. Each square represents the mean of 3 replicates ( n = 3 independent cultures). ( B ) L929 cells were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) stimulation for 6 hours in the absence or presence of 20 mM aspartate or asparagine, and then cells were harvested for qPCR analysis of IFN response gene expression. ( C ) Heatmap from RNA-seq revealing the expression of ISGs in L929 cells with indicated treatment compared with mock group [ z -score–normalized log 2 (fold per million reads) values, n = 3 independent cultures]. ( D ) GSEA of interferon-beta response gene. ( E ) Western blot detected p-IRF3 and p-STAT1 levels in L929 cells treated as indicated. ( F ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection in the absence or presence of 20 mM aspartate. Scale bars, 5 μm. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( B ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Figure Legend Snippet: ( A ) Heatmap of metabolite changes in mock- and AOA-treated L929 cells followed with HT-DNA transfection or without HT-DNA transfection. The fold-change of metabolites abundances was normalized to the mock group. Each square represents the mean of 3 replicates ( n = 3 independent cultures). ( B ) L929 cells were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) stimulation for 6 hours in the absence or presence of 20 mM aspartate or asparagine, and then cells were harvested for qPCR analysis of IFN response gene expression. ( C ) Heatmap from RNA-seq revealing the expression of ISGs in L929 cells with indicated treatment compared with mock group [ z -score–normalized log 2 (fold per million reads) values, n = 3 independent cultures]. ( D ) GSEA of interferon-beta response gene. ( E ) Western blot detected p-IRF3 and p-STAT1 levels in L929 cells treated as indicated. ( F ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection in the absence or presence of 20 mM aspartate. Scale bars, 5 μm. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( B ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Techniques Used: Transfection, Gene Expression, RNA Sequencing, Expressing, Western Blot, Immunofluorescence

$( A ) Schematic showing that aspartate-mediated pyrimidine nucleotide synthesis is essential for intracellular DNA homeostasis. ( B ) Heatmap from RNA-seq revealing the expression of DNA repair enzymes in L929 cells with indicated treatment. The fold-change of expression was normalized to the mock group ( n = 3 independent cultures). ( C ) Genome browser screenshots of END-seq on mitochondria from L929 cells with indicated treatment. The expanded view of the indicated region shows more detailed genomic features. ( D ) The proportion of END-seq reads in mitochondrial and nuclear chromosomal regions. ( E ) Representative immunofluorescence images of mitochondrial (TOM20, red) or dsDNA (green) in BJ-5ta cells treated with AOA in the absence or presence of 20 mM aspartate for 6 hours. Scale bars, 10 μm. Lower panel: quantification of cytosolic dsDNA in BJ-5ta cells with indicated treatment ( n = 39–51 fields per group from 3 biological replicates). ( F ) Representative immunofluorescence images of BJ-5ta cells treated with ddC (100 μM) for 72 hours, followed by 6 hours of AOA treatment in the absence or presence of 20 mM aspartate. ( G ) 2D–structured illumination microscopy imaging of mtDNA externalization under the indicated conditions. ( H ) Western blot detected α-tubulin (cytosol), TOM20 (OMM), PDH (Matrix), COX IV (IMM), and histone H3 (nuclei) to validate protocol from in L929 cells treated as indicated. wcl, whole cell lysate; pel, pellet; cyt, cytosolic fraction. ( I ) L929 cells were treated with AOA in the absence or presence of 20 mM aspartate followed by HT-DNA transfection, and then cells were harvested for qPCR analysis of mtDNA or nDNA levels in cytosolic fractions. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( E and I ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. DSBs, double-strand breaks.$
Figure Legend Snippet: ( A ) Schematic showing that aspartate-mediated pyrimidine nucleotide synthesis is essential for intracellular DNA homeostasis. ( B ) Heatmap from RNA-seq revealing the expression of DNA repair enzymes in L929 cells with indicated treatment. The fold-change of expression was normalized to the mock group ( n = 3 independent cultures). ( C ) Genome browser screenshots of END-seq on mitochondria from L929 cells with indicated treatment. The expanded view of the indicated region shows more detailed genomic features. ( D ) The proportion of END-seq reads in mitochondrial and nuclear chromosomal regions. ( E ) Representative immunofluorescence images of mitochondrial (TOM20, red) or dsDNA (green) in BJ-5ta cells treated with AOA in the absence or presence of 20 mM aspartate for 6 hours. Scale bars, 10 μm. Lower panel: quantification of cytosolic dsDNA in BJ-5ta cells with indicated treatment ( n = 39–51 fields per group from 3 biological replicates). ( F ) Representative immunofluorescence images of BJ-5ta cells treated with ddC (100 μM) for 72 hours, followed by 6 hours of AOA treatment in the absence or presence of 20 mM aspartate. ( G ) 2D–structured illumination microscopy imaging of mtDNA externalization under the indicated conditions. ( H ) Western blot detected α-tubulin (cytosol), TOM20 (OMM), PDH (Matrix), COX IV (IMM), and histone H3 (nuclei) to validate protocol from in L929 cells treated as indicated. wcl, whole cell lysate; pel, pellet; cyt, cytosolic fraction. ( I ) L929 cells were treated with AOA in the absence or presence of 20 mM aspartate followed by HT-DNA transfection, and then cells were harvested for qPCR analysis of mtDNA or nDNA levels in cytosolic fractions. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( E and I ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. DSBs, double-strand breaks.

Techniques Used: RNA Sequencing, Expressing, Immunofluorescence, Microscopy, Imaging, Western Blot, Transfection

Cell Culture:

Article Title: From phytochemical complexity to biological function: A comparative study of Capparis spinosa fruit extracts.
Article Snippet: Capparis spinosa L. is a Mediterranean species traditionally used for the treatment of inflammatory and skinrelated disorders and increasingly explored as a source of multifunctional ingredients for dermocosmetic applications.. In this study, three commercially available C. spinosa fruit extracts obtained through different extraction strategies (two aqueous extracts, CAP1 and CAP2, and one hydroalcoholic extract, CAP3) were comparatively investigated using an integrated phytochemical and biological approach.. Phytochemical profiling

Article Title: Genome-wide mapping of Cas9-induced sister chromatid exchange across single and over 200 genomic targets
Article Snippet: .. BJ-5ta cells (CRL-4001, ATCC) were cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS) and 1× penicillin-streptomycin at 37 °C in 5% CO 2 . .. Cas9 ribonucleoprotein (RNP) complexes were assembled using Alt-R S. pyogenes Cas9 Nuclease V3 (1081058; IDT) or corresponding nickase variants (Alt-R Cas9 D10A Nickase V3; Alt-R Cas9 H840A Nickase V3) together with Alt-R tracrRNA (1072532; IDT) and Alt-R crRNA.

Article Title: Lesions initiating spontaneous mitotic crossover are minimally subject to non-homologous end joining
Article Snippet: .. BJ-5ta (CRL-4001, ATCC) cells were cultured in DMEM/F12 medium supplemented with 10% FBS and 1x Pen-Strep. ..

Article Title: Constant cGAS activation blunts STING signaling in DU145 prostate cancer cell line.
Article Snippet: .. Cell culture. hTERT-BJ-5ta cells (human skin fibroblast cells, CRL-4001; American Type Culture Collection [ATCC]), MCF7 cells (human breast adenocarcinoma cells, HTB-22; ATCC), U2OS cells (human osteosarcoma cells; gift from T. Hirota), A549 cells (human lung adenocarcinoma cells, CCL-185; ATCC), DU145 cells (human prostate carcinoma cells, HTB-81; ATCC), HCT116 cells (human colorectal carcinoma cells, CCL-247; ATCC), HCT15 cells and DLD-1 cells (human colorectal carcinoma cells, TKG 0504 and TKG0379; Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Miyagi, Japan) were grown at 37°C in a 5% CO2 atmosphere in DMEM (Nacalai Tescue), supplemented with 10% fetal bovine serum (FBS, Serana) and penicillin/streptomycin Mixed Solution (Nacalai Tescue). ..

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Journal: The Journal of Clinical Investigation

Article Title: Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity

doi: 10.1172/JCI199716

Figure Lengend Snippet: ( A ) Outline of metabolic inhibitors for screening. ( B ) Human hTERT-immortalized foreskin fibroblast (BJ-5ta, left) and mouse fibroblast (L929, right) cells were treated with inhibitors from A followed by 0.5 μg/mL HT-DNA transfection for 5 hours. Cells were harvested for qPCR analysis of human IFNB or mouse Cxcl10 . ( C ) BJ-5ta cells were treated with 1 mM AOA for 1 hour followed by HT-DNA (0.5 μg/mL) transfection for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( D and E ) L929 ( D ) and MC38 ( E ) were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) or HT-DNA (0.5 μg/mL) stimulation for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( F – H ) Western blot detected phosphorylated (p-) p-IRF3 and p-STAT1 levels in BJ-5ta, L929, and MC38 cells treated as in C – E . ( I ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection or not. Scale bars, 5 μm. ( J ) Schematic of the main targets of AOA. ( K ) Western blot detected p-IRF3, p-STAT1, GOT2, and GPT2 levels in BJ-5ta cells treated as indicated. ( L ) The relative IFNB and CXCL10 mRNA expression in the control BJ-5ta cells versus GOT2 and GPT2 siRNA-silenced BJ-5ta cells after indicated treatment. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by unpaired t test ( C – E , I , and L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Six- to 8-week-old C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd, and age-matched Ifnar1 -KO C57BL/6J mice were obtained from Cyagen Biosciences (Suzhou) Inc. BJ-5ta and L929 cells were obtained from ATCC, while B16-F10 and MC38 cells were purchased from the National Collection of Authenticated Cell Cultures.

Techniques: Transfection, Gene Expression, Western Blot, Immunofluorescence, Expressing, Control

Journal: The Journal of Clinical Investigation

Article Title: Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity

doi: 10.1172/JCI199716

Figure Lengend Snippet: ( A ) Heatmap of metabolite changes in mock- and AOA-treated L929 cells followed with HT-DNA transfection or without HT-DNA transfection. The fold-change of metabolites abundances was normalized to the mock group. Each square represents the mean of 3 replicates ( n = 3 independent cultures). ( B ) L929 cells were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) stimulation for 6 hours in the absence or presence of 20 mM aspartate or asparagine, and then cells were harvested for qPCR analysis of IFN response gene expression. ( C ) Heatmap from RNA-seq revealing the expression of ISGs in L929 cells with indicated treatment compared with mock group [ z -score–normalized log 2 (fold per million reads) values, n = 3 independent cultures]. ( D ) GSEA of interferon-beta response gene. ( E ) Western blot detected p-IRF3 and p-STAT1 levels in L929 cells treated as indicated. ( F ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection in the absence or presence of 20 mM aspartate. Scale bars, 5 μm. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( B ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Techniques: Transfection, Gene Expression, RNA Sequencing, Expressing, Western Blot, Immunofluorescence

Journal: The Journal of Clinical Investigation

Article Title: Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity

doi: 10.1172/JCI199716

Figure Lengend Snippet: ( A ) Schematic showing that aspartate-mediated pyrimidine nucleotide synthesis is essential for intracellular DNA homeostasis. ( B ) Heatmap from RNA-seq revealing the expression of DNA repair enzymes in L929 cells with indicated treatment. The fold-change of expression was normalized to the mock group ( n = 3 independent cultures). ( C ) Genome browser screenshots of END-seq on mitochondria from L929 cells with indicated treatment. The expanded view of the indicated region shows more detailed genomic features. ( D ) The proportion of END-seq reads in mitochondrial and nuclear chromosomal regions. ( E ) Representative immunofluorescence images of mitochondrial (TOM20, red) or dsDNA (green) in BJ-5ta cells treated with AOA in the absence or presence of 20 mM aspartate for 6 hours. Scale bars, 10 μm. Lower panel: quantification of cytosolic dsDNA in BJ-5ta cells with indicated treatment ( n = 39–51 fields per group from 3 biological replicates). ( F ) Representative immunofluorescence images of BJ-5ta cells treated with ddC (100 μM) for 72 hours, followed by 6 hours of AOA treatment in the absence or presence of 20 mM aspartate. ( G ) 2D–structured illumination microscopy imaging of mtDNA externalization under the indicated conditions. ( H ) Western blot detected α-tubulin (cytosol), TOM20 (OMM), PDH (Matrix), COX IV (IMM), and histone H3 (nuclei) to validate protocol from in L929 cells treated as indicated. wcl, whole cell lysate; pel, pellet; cyt, cytosolic fraction. ( I ) L929 cells were treated with AOA in the absence or presence of 20 mM aspartate followed by HT-DNA transfection, and then cells were harvested for qPCR analysis of mtDNA or nDNA levels in cytosolic fractions. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( E and I ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. DSBs, double-strand breaks.

Techniques: RNA Sequencing, Expressing, Immunofluorescence, Microscopy, Imaging, Western Blot, Transfection

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bj 5ta (ATCC)

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