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bd4  (Addgene inc)
93
Addgene inc bd4
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Bd4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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39103 03 biolock iba lifesciences cat  (IBA Lifesciences)
96
IBA Lifesciences 39103 03 biolock iba lifesciences cat
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
39103 03 Biolock Iba Lifesciences Cat, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacillus amyloliquefaciens subsp plantarum ucmb5036  (ATCC)
90
ATCC bacillus amyloliquefaciens subsp plantarum ucmb5036
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Bacillus Amyloliquefaciens Subsp Plantarum Ucmb5036, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pnic cthf vector  (Addgene inc)
93
Addgene inc pnic cthf vector
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Pnic Cthf Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid numbers 38999 39013 39027 39028 39030 39103  (Addgene inc)
93
Addgene inc plasmid numbers 38999 39013 39027 39028 39030 39103
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Plasmid Numbers 38999 39013 39027 39028 39030 39103, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbrm1 bd4  (Addgene inc)
93
Addgene inc pbrm1 bd4
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Pbrm1 Bd4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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salmonella abony ihe 103 39  (ATCC)
90
ATCC salmonella abony ihe 103 39
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Salmonella Abony Ihe 103 39, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jnk3 srebp2 sirna  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology jnk3 srebp2 sirna
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Jnk3 Srebp2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gpi  (ATCC)
90
ATCC gpi
(A) Coomassie gel of the purified recombinant proteins BD2, BD3, <t>BD4,</t> BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .
Gpi, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Coomassie gel of the purified recombinant proteins BD2, BD3, BD4, BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .

Journal: bioRxiv

Article Title: PBRM1-Dependent PBAF Targeting is Required for EMT and Metastasis in Breast Cancer

doi: 10.1101/2025.10.19.683137

Figure Lengend Snippet: (A) Coomassie gel of the purified recombinant proteins BD2, BD3, BD4, BD5 and the tandem BD2-5 used for peptide and nucleosome binding assays. (B) Schematic representation of EpiCypher’s Captify™ assay. (C) Table of EC 50 values (nM) of the different BDs for the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. (D) Binding curves of tandem BD2-5 with the indicated peptides obtained using the ALPHA/dCypher assay. EC 50 (nM) values are indicated next to the corresponding curves. (E) Table of the relative EC 50 values (nM) of the different BDs for nucleosomes bearing the indicated histone modifications obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 nucleosomes is used as a positive control in this assay. (F) Binding curves of tandem BD2-5 for nucleosomes bearing the indicated peptides obtained using the ALPHA/dCypher assay. HP1 binding to H3K9me3 peptide is used as a positive control in this assay. EC 50 values (nM) obtained for positive binders are indicated in the legend. (G and H) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac at Phf10 binding sites in untreated (H) and 48h TGFβ1-treated (I) sgCt cells. (I) Correlation matrix with r-values between Phf10 and H3K14ac, H3K18ac, H3K27ac, and H3K4me3 ChIP-seq enrichment in untreated and 48h TGFβ1-treated sgCt cells. (J) Metagene plots and heatmaps of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated and 48h TGFβ1-treated sgCt cells. The top heatmap is at Phf10 binding sites in untreated cells and the bottom is Phf10 binding sites only found in TGFβ1-treated cells. (K and L) Genomic tracks of ChIP-seq enrichment of Phf10, H3K14ac, H3K18ac, and H3K27ac in untreated (M) and 48h-TGFβ1 treated (M) sgCt cells at constitutive locus Cpne2 and an inducible locus Tnfsf13b .

Article Snippet: Constructs encoding codon-optimized ORFs for bacterial expression of human PBRM1 BD2 (addgene #39013), BD3 (addgene #39030), BD4 (addgene #39103), BD5 (addgene #38999) and tandem BD2-5 (SGC construct ID #PB1A-c080) were transformed in BL21(DE3) for BDs, and BL21 Rosetta2 (DE3) pLysS for tandem BD2-5.

Techniques: Purification, Recombinant, Binding Assay, Positive Control, ChIP-sequencing