39103 Search Results


recombinant pbrm1  (Addgene inc)
93
Addgene inc recombinant pbrm1
Recombinant Pbrm1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/39103/pmc05314159-663-0-11?v=Addgene+inc
Average 93 stars, based on 1 article reviews
recombinant pbrm1 - by Bioz Stars, 2026-08
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sirna for jnk  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology sirna for jnk
Figure 6. PI3K/Akt and mTOR signaling in R-Ras–transformed cells. A, cell populations were analyzed for levels of activated and total Akt and mTOR by Western blot analysis. Serum-starved cells were stimulated with TGF-h (5 ng/mL) for the times indicated, and immunoblots were performed on 20 Ag of total cell lysate using the antibodies indicated. A representative of three experiments performed is shown. B, gene silencing was performed on cell populations using <t>siRNA.</t> Shown are cell populations either nontransfected (NT), transfected with siRNA targeting either PI3Kh, Akt, or mTOR (Target), or transfected with a scrambled control (Scram). Immunoblots were performed on 20 Ag of total cell lysate to determine levels of target gene expression using the antibodies indicated. C, soft agar colony formation assays were performed using knockdown R-Ras/EpH4 cells and colony formation was scored after 9 d. Knockdown of PI3K, Akt, or mTOR expression significantly reduced colony formation in R-Ras/EpH4 cells. *, P < 0.001. D, 72-h cell proliferation assays were performed using knockdown cells. Silencing PI3K, Akt, or mTOR expression significantly reduced R-Ras/EpH4 cell proliferation. *, P < 0.01. Knockdown of PI3K or Akt significantly reduced proliferation in control cells. **, P < 0.05. Columns, mean values from transformation and proliferation assays from triplicate wells of a representative experiment; bars, SD.
Sirna For Jnk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/39103/10__1158_slash_0008___5472__can___08___0513-54-1-32?v=Santa+Cruz+Biotechnology
Average 91 stars, based on 1 article reviews
sirna for jnk - by Bioz Stars, 2026-08
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coculture  (ATCC)
90
ATCC coculture
Figure 6. PI3K/Akt and mTOR signaling in R-Ras–transformed cells. A, cell populations were analyzed for levels of activated and total Akt and mTOR by Western blot analysis. Serum-starved cells were stimulated with TGF-h (5 ng/mL) for the times indicated, and immunoblots were performed on 20 Ag of total cell lysate using the antibodies indicated. A representative of three experiments performed is shown. B, gene silencing was performed on cell populations using <t>siRNA.</t> Shown are cell populations either nontransfected (NT), transfected with siRNA targeting either PI3Kh, Akt, or mTOR (Target), or transfected with a scrambled control (Scram). Immunoblots were performed on 20 Ag of total cell lysate to determine levels of target gene expression using the antibodies indicated. C, soft agar colony formation assays were performed using knockdown R-Ras/EpH4 cells and colony formation was scored after 9 d. Knockdown of PI3K, Akt, or mTOR expression significantly reduced colony formation in R-Ras/EpH4 cells. *, P < 0.001. D, 72-h cell proliferation assays were performed using knockdown cells. Silencing PI3K, Akt, or mTOR expression significantly reduced R-Ras/EpH4 cell proliferation. *, P < 0.01. Knockdown of PI3K or Akt significantly reduced proliferation in control cells. **, P < 0.05. Columns, mean values from transformation and proliferation assays from triplicate wells of a representative experiment; bars, SD.
Coculture, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/39103/10__1128_slash_cvi__00648___14-103-1-36?v=ATCC
Average 90 stars, based on 1 article reviews
coculture - by Bioz Stars, 2026-08
90/100 stars
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a protophormiae  (ATCC)
90
ATCC a protophormiae
Figure 6. PI3K/Akt and mTOR signaling in R-Ras–transformed cells. A, cell populations were analyzed for levels of activated and total Akt and mTOR by Western blot analysis. Serum-starved cells were stimulated with TGF-h (5 ng/mL) for the times indicated, and immunoblots were performed on 20 Ag of total cell lysate using the antibodies indicated. A representative of three experiments performed is shown. B, gene silencing was performed on cell populations using <t>siRNA.</t> Shown are cell populations either nontransfected (NT), transfected with siRNA targeting either PI3Kh, Akt, or mTOR (Target), or transfected with a scrambled control (Scram). Immunoblots were performed on 20 Ag of total cell lysate to determine levels of target gene expression using the antibodies indicated. C, soft agar colony formation assays were performed using knockdown R-Ras/EpH4 cells and colony formation was scored after 9 d. Knockdown of PI3K, Akt, or mTOR expression significantly reduced colony formation in R-Ras/EpH4 cells. *, P < 0.001. D, 72-h cell proliferation assays were performed using knockdown cells. Silencing PI3K, Akt, or mTOR expression significantly reduced R-Ras/EpH4 cell proliferation. *, P < 0.01. Knockdown of PI3K or Akt significantly reduced proliferation in control cells. **, P < 0.05. Columns, mean values from transformation and proliferation assays from triplicate wells of a representative experiment; bars, SD.
A Protophormiae, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/39103/pm11807567-138-85-90?v=ATCC
Average 90 stars, based on 1 article reviews
a protophormiae - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Figure 6. PI3K/Akt and mTOR signaling in R-Ras–transformed cells. A, cell populations were analyzed for levels of activated and total Akt and mTOR by Western blot analysis. Serum-starved cells were stimulated with TGF-h (5 ng/mL) for the times indicated, and immunoblots were performed on 20 Ag of total cell lysate using the antibodies indicated. A representative of three experiments performed is shown. B, gene silencing was performed on cell populations using siRNA. Shown are cell populations either nontransfected (NT), transfected with siRNA targeting either PI3Kh, Akt, or mTOR (Target), or transfected with a scrambled control (Scram). Immunoblots were performed on 20 Ag of total cell lysate to determine levels of target gene expression using the antibodies indicated. C, soft agar colony formation assays were performed using knockdown R-Ras/EpH4 cells and colony formation was scored after 9 d. Knockdown of PI3K, Akt, or mTOR expression significantly reduced colony formation in R-Ras/EpH4 cells. *, P < 0.001. D, 72-h cell proliferation assays were performed using knockdown cells. Silencing PI3K, Akt, or mTOR expression significantly reduced R-Ras/EpH4 cell proliferation. *, P < 0.01. Knockdown of PI3K or Akt significantly reduced proliferation in control cells. **, P < 0.05. Columns, mean values from transformation and proliferation assays from triplicate wells of a representative experiment; bars, SD.

Journal: Cancer Research

Article Title: Transforming Growth Factor-β (TGF-β) and TGF-β–Associated Kinase 1 Are Required for R-Ras–Mediated Transformation of Mammary Epithelial Cells

doi: 10.1158/0008-5472.can-08-0513

Figure Lengend Snippet: Figure 6. PI3K/Akt and mTOR signaling in R-Ras–transformed cells. A, cell populations were analyzed for levels of activated and total Akt and mTOR by Western blot analysis. Serum-starved cells were stimulated with TGF-h (5 ng/mL) for the times indicated, and immunoblots were performed on 20 Ag of total cell lysate using the antibodies indicated. A representative of three experiments performed is shown. B, gene silencing was performed on cell populations using siRNA. Shown are cell populations either nontransfected (NT), transfected with siRNA targeting either PI3Kh, Akt, or mTOR (Target), or transfected with a scrambled control (Scram). Immunoblots were performed on 20 Ag of total cell lysate to determine levels of target gene expression using the antibodies indicated. C, soft agar colony formation assays were performed using knockdown R-Ras/EpH4 cells and colony formation was scored after 9 d. Knockdown of PI3K, Akt, or mTOR expression significantly reduced colony formation in R-Ras/EpH4 cells. *, P < 0.001. D, 72-h cell proliferation assays were performed using knockdown cells. Silencing PI3K, Akt, or mTOR expression significantly reduced R-Ras/EpH4 cell proliferation. *, P < 0.01. Knockdown of PI3K or Akt significantly reduced proliferation in control cells. **, P < 0.05. Columns, mean values from transformation and proliferation assays from triplicate wells of a representative experiment; bars, SD.

Article Snippet: Pooled siRNA for JNK (targeting JNK1, JNK2, and JNK3) and specific siRNA for p38 MAPK, mTOR (FRAP), TBRII, TAK1, PI3K (targeting the p110h subunit), Smad2/3, and scrambled control siRNAs were obtained from Santa Cruz Biotechnology, Inc. and transfected using the manufacturer’s reagents and protocol.

Techniques: Transformation Assay, Western Blot, Transfection, Control, Targeted Gene Expression, Knockdown, Expressing