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mycobacterium bovis variant af2122  (ATCC)


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    Structured Review

    ATCC mycobacterium bovis variant af2122
    Mycobacterium Bovis Variant Af2122, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/35744/Mycobacterium+tuberculosis+variant+bovis/pm38029724-171-13-23
    Average 92 stars, based on 15 article reviews
    mycobacterium bovis variant af2122 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Variant Assay:

    Article Title: Intramuscular Boosting with hIFN-Alpha 2b Enhances BCGphipps-Induced Protection in a Murine Model of Leprosy
    Article Snippet: .. The BCG Phipps strain (ATCC® 35744TM) (BCGph) is a variant of the BCG strain that has lost the region RD1 which encodes ESAT-6 and CFP10, with the lowest number of T cell epitopes, 316, versus BCG Tokyo, 359, and BCG Pasteur, 331, (Zhang et al., 2013) [51] and was provided by Dr. Hernández-Pando (INCMNSZ, Mexico City, MX). ..

    Article Title: Association between mutations in a thyX-hsdS.1 region and para -aminosalicylic acid resistance in Mycobacterium tuberculosis clinical isolates
    Article Snippet: .. Mtb, Mycobacterium tuberculosis H37Rv; Msm, Mycobacterium smegmatis MC 2 155; Mma, Mycobacterium marinum M; Mbo, Mycobacterium bovis variant AF2122-97; Mha, Mycobacterium haemophilum DSM 44634 strain ATCC 29548; Mlpm, Mycobacterium lepromatosis strain Mx1-22A; Mlp, Mycobacterium leprae TN; Mab, Mycobacterium abscessus strain ATCC 19977. ..

    Article Title: Gamma-aminobutyric acid type A receptor alpha 4 coordinates autophagy, inflammation, and immunometabolism to promote innate immune activation
    Article Snippet: .. Mtb H37Rv was kindly provided by Dr. R. L. Friedman of the University of Arizona, M. bovis BCG was from the Korean Institute of Tuberculosis, and the smooth variant of Mabc was from ATCC (19977). .. They were grown in Middlebrook 7H9 broth (Difco, 271310) containing 0.5% glycerol, 0.005% Tween-80, and oleic albumin dextrose catalase (OADC; BD Biosciences, 212240).

    Article Title: Genome sequences of BCG Pasteur ATCC 35734 and its derivative, the vaccine candidate BCGΔBCG1419c
    Article Snippet: .. The raw sequence reads can be found as CP110223.1 - Mycobacterium tuberculosis variant bovis strain BCG delta BCG1419c mutant chromosome. https://www.ncbi.nlm.nih.gov/nuccore/CP110223 , and CP109681.1 - Mycobacterium tuberculosis variant bovis strain BCG Pasteur ATCC 35,734 chromosome https://www.ncbi.nlm.nih.gov/nuccore/CP109681 . ..

    Article Title: Association between mutations in a thyX-hsdS.1 region and para -aminosalicylic acid resistance in Mycobacterium tuberculosis clinical isolates.
    Article Snippet: .. Mtb, Mycobacterium tuberculosis H37Rv; Msm, Mycobacterium smegmatis MC2155; Mma, Mycobacterium marinum M; Mbo, Mycobacterium bovis variant AF2122-97; Mha, Mycobacterium haemophilum DSM 44634 strain ATCC 29548; Mlpm, Mycobacterium lepromatosis strain Mx1-22A; Mlp, Mycobacterium leprae TN; Mab, Mycobacterium abscessus strain ATCC 19977. ..

    Sequencing:

    Article Title: Genome sequences of BCG Pasteur ATCC 35734 and its derivative, the vaccine candidate BCGΔBCG1419c
    Article Snippet: .. The raw sequence reads can be found as CP110223.1 - Mycobacterium tuberculosis variant bovis strain BCG delta BCG1419c mutant chromosome. https://www.ncbi.nlm.nih.gov/nuccore/CP110223 , and CP109681.1 - Mycobacterium tuberculosis variant bovis strain BCG Pasteur ATCC 35,734 chromosome https://www.ncbi.nlm.nih.gov/nuccore/CP109681 . ..

    Mutagenesis:

    Article Title: Genome sequences of BCG Pasteur ATCC 35734 and its derivative, the vaccine candidate BCGΔBCG1419c
    Article Snippet: .. The raw sequence reads can be found as CP110223.1 - Mycobacterium tuberculosis variant bovis strain BCG delta BCG1419c mutant chromosome. https://www.ncbi.nlm.nih.gov/nuccore/CP110223 , and CP109681.1 - Mycobacterium tuberculosis variant bovis strain BCG Pasteur ATCC 35,734 chromosome https://www.ncbi.nlm.nih.gov/nuccore/CP109681 . ..



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    ATCC cp110223 1 mycobacterium tuberculosis variant bovis strain bcg delta bcg1419c mutant chromosome
    Schematic representarion showing the genomic region upstream and downstream the deletion of the <t>BCG1419c</t> gene. The upper panels shows the region present in wild type BCG Pasteur ATCC 35734, the middle panel, the region present in BCG?BCG1419c, and the bottom panel shows gene names when they have an annotation available.
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    ATCC bcg substrains phipps
    Analyses of the outermost surface of <t>BCG</t> <t>substrains.</t> ( A , B ) Thin-layer chromatography (TLC) corresponding to the superficial lipids extracted using petroleum ether (PE) at 40–60 °C for 5 min of BCG substrain pellicles grown on A60 and G15 Sauton media. The volume of lipid extracts applied to TLC plates was equal under all conditions and derived from the same pellicle surface. The results of one representative experiment out of at least three biological replicates. TLC elution was performed ( A ) in PE:diethyl ether at 90:10 ( v / v ) or ( B ) in chloroform:methanol at 96:4 ( v / v ). Both plates were treated with 10% molybdatophosphoric acid in ethanol. BCG-Pasteur (Lines 1 and 1′); <t>BCG-Phipps</t> (Lines 2 and 2′); BCG-Tice (Lines 3 and 3′); BCG-Glaxo (Lines 4 and 4′); and BCG-Moreau (Lines 5 and 5′). 1-5 BCG substrains grown on A60 Sauton medium and 1′-5′ BCG substrains grown on G15 Sauton medium. ( C ) Fold ratio of papA5 and ppsA expression in BCG substrains grown on A60 medium relative to that of BCG substrains grown on G15 medium. ( D ) Scheme of genes involved in PDIM and PGL synthesis. ( E ) Affinity of BCG substrains towards hexadecane, E. coli was used as a negative control. The results are expressed as a percentage of the initial absorbance at 400 nm. appearance. Data represent the mean ± standard deviation (SD) from three independent experiments. * p < 0.05, **** p <0.0001 (ANOVA test).
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    Image Search Results


    Schematic representarion showing the genomic region upstream and downstream the deletion of the BCG1419c gene. The upper panels shows the region present in wild type BCG Pasteur ATCC 35734, the middle panel, the region present in BCG?BCG1419c, and the bottom panel shows gene names when they have an annotation available.

    Journal: BMC Genomics

    Article Title: Genome sequences of BCG Pasteur ATCC 35734 and its derivative, the vaccine candidate BCGΔBCG1419c

    doi: 10.1186/s12864-023-09169-9

    Figure Lengend Snippet: Schematic representarion showing the genomic region upstream and downstream the deletion of the BCG1419c gene. The upper panels shows the region present in wild type BCG Pasteur ATCC 35734, the middle panel, the region present in BCG?BCG1419c, and the bottom panel shows gene names when they have an annotation available.

    Article Snippet: The raw sequence reads can be found as CP110223.1 - Mycobacterium tuberculosis variant bovis strain BCG delta BCG1419c mutant chromosome. https://www.ncbi.nlm.nih.gov/nuccore/CP110223 , and CP109681.1 - Mycobacterium tuberculosis variant bovis strain BCG Pasteur ATCC 35,734 chromosome https://www.ncbi.nlm.nih.gov/nuccore/CP109681 .

    Techniques:

    Analyses of the outermost surface of BCG substrains. ( A , B ) Thin-layer chromatography (TLC) corresponding to the superficial lipids extracted using petroleum ether (PE) at 40–60 °C for 5 min of BCG substrain pellicles grown on A60 and G15 Sauton media. The volume of lipid extracts applied to TLC plates was equal under all conditions and derived from the same pellicle surface. The results of one representative experiment out of at least three biological replicates. TLC elution was performed ( A ) in PE:diethyl ether at 90:10 ( v / v ) or ( B ) in chloroform:methanol at 96:4 ( v / v ). Both plates were treated with 10% molybdatophosphoric acid in ethanol. BCG-Pasteur (Lines 1 and 1′); BCG-Phipps (Lines 2 and 2′); BCG-Tice (Lines 3 and 3′); BCG-Glaxo (Lines 4 and 4′); and BCG-Moreau (Lines 5 and 5′). 1-5 BCG substrains grown on A60 Sauton medium and 1′-5′ BCG substrains grown on G15 Sauton medium. ( C ) Fold ratio of papA5 and ppsA expression in BCG substrains grown on A60 medium relative to that of BCG substrains grown on G15 medium. ( D ) Scheme of genes involved in PDIM and PGL synthesis. ( E ) Affinity of BCG substrains towards hexadecane, E. coli was used as a negative control. The results are expressed as a percentage of the initial absorbance at 400 nm. appearance. Data represent the mean ± standard deviation (SD) from three independent experiments. * p < 0.05, **** p <0.0001 (ANOVA test).

    Journal: Vaccines

    Article Title: BCG Substrains Change Their Outermost Surface as a Function of Growth Media

    doi: 10.3390/vaccines10010040

    Figure Lengend Snippet: Analyses of the outermost surface of BCG substrains. ( A , B ) Thin-layer chromatography (TLC) corresponding to the superficial lipids extracted using petroleum ether (PE) at 40–60 °C for 5 min of BCG substrain pellicles grown on A60 and G15 Sauton media. The volume of lipid extracts applied to TLC plates was equal under all conditions and derived from the same pellicle surface. The results of one representative experiment out of at least three biological replicates. TLC elution was performed ( A ) in PE:diethyl ether at 90:10 ( v / v ) or ( B ) in chloroform:methanol at 96:4 ( v / v ). Both plates were treated with 10% molybdatophosphoric acid in ethanol. BCG-Pasteur (Lines 1 and 1′); BCG-Phipps (Lines 2 and 2′); BCG-Tice (Lines 3 and 3′); BCG-Glaxo (Lines 4 and 4′); and BCG-Moreau (Lines 5 and 5′). 1-5 BCG substrains grown on A60 Sauton medium and 1′-5′ BCG substrains grown on G15 Sauton medium. ( C ) Fold ratio of papA5 and ppsA expression in BCG substrains grown on A60 medium relative to that of BCG substrains grown on G15 medium. ( D ) Scheme of genes involved in PDIM and PGL synthesis. ( E ) Affinity of BCG substrains towards hexadecane, E. coli was used as a negative control. The results are expressed as a percentage of the initial absorbance at 400 nm. appearance. Data represent the mean ± standard deviation (SD) from three independent experiments. * p < 0.05, **** p <0.0001 (ANOVA test).

    Article Snippet: The BCG substrains Phipps (ATCC 35744) and Tice (ATCC 27289) strains were purchased from the Spanish Type Culture Collection and from the German Collection of Microorganisms and Cell Cultures, respectively.

    Techniques: Thin Layer Chromatography, Derivative Assay, Expressing, Negative Control, Standard Deviation

    Superficial lipidic pattern of  BCG  substrains (Pasteur, Phipps, Tice, Glaxo, and Moreau) grown on two different Sauton compositions (A60 and G15) that differ by amino acid source, L-asparagine (A) or L-glutamate (G), and the glycerol concentration, 60 mL/L (60) or 15 mL/L (15). PDIM: phthiocerol dimycocerosate; AG: acylglycerol; PGL: phenolic glycolipid; GroMM: glycerol monomycolate. +, present; ++, highly present; +++, abundantly present; -, not present.

    Journal: Vaccines

    Article Title: BCG Substrains Change Their Outermost Surface as a Function of Growth Media

    doi: 10.3390/vaccines10010040

    Figure Lengend Snippet: Superficial lipidic pattern of BCG substrains (Pasteur, Phipps, Tice, Glaxo, and Moreau) grown on two different Sauton compositions (A60 and G15) that differ by amino acid source, L-asparagine (A) or L-glutamate (G), and the glycerol concentration, 60 mL/L (60) or 15 mL/L (15). PDIM: phthiocerol dimycocerosate; AG: acylglycerol; PGL: phenolic glycolipid; GroMM: glycerol monomycolate. +, present; ++, highly present; +++, abundantly present; -, not present.

    Article Snippet: The BCG substrains Phipps (ATCC 35744) and Tice (ATCC 27289) strains were purchased from the Spanish Type Culture Collection and from the German Collection of Microorganisms and Cell Cultures, respectively.

    Techniques: Concentration Assay

    Neutral red staining and Rv0577 gene expression of BCG substrains. ( A ) Images corresponding to neutral red staining in cells of BCG substrains grown on different culture media. ( B ) Fold change of Rv0577 expression in BCG substrains grown on A60 medium relative to that of BCG substrains grown on G15 medium. Two independent pellicles for each condition were analyzed.

    Journal: Vaccines

    Article Title: BCG Substrains Change Their Outermost Surface as a Function of Growth Media

    doi: 10.3390/vaccines10010040

    Figure Lengend Snippet: Neutral red staining and Rv0577 gene expression of BCG substrains. ( A ) Images corresponding to neutral red staining in cells of BCG substrains grown on different culture media. ( B ) Fold change of Rv0577 expression in BCG substrains grown on A60 medium relative to that of BCG substrains grown on G15 medium. Two independent pellicles for each condition were analyzed.

    Article Snippet: The BCG substrains Phipps (ATCC 35744) and Tice (ATCC 27289) strains were purchased from the Spanish Type Culture Collection and from the German Collection of Microorganisms and Cell Cultures, respectively.

    Techniques: Staining, Gene Expression, Expressing