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Viruses characterized and associated stability values.

Journal: PLoS Pathogens

Article Title: Relationship between hemagglutinin stability and influenza virus persistence after exposure to low pH or supraphysiological heating

doi: 10.1371/journal.ppat.1009910

Figure Lengend Snippet: Viruses characterized and associated stability values.

Article Snippet: 14 , A/Florida/3/2006 (H1N1) , human , 5.30 , 5.20 , , 0.10 , , ATCC VR-1893.

Techniques: Activation Assay, Virus

TCID50 values (left y-axis, black circles) and luciferase activity (RLU, right y-axis, white squares) were measured as a function of exposure to the reported pH followed by re-neutralization. Right y-axes are scaled so that RLU maxima and minima are displayed at levels similar to those from TCID50 on the opposite axis. Error bars represent standard deviation (n = 3). Reported data are representative of two or more independent experiments. (A) rg-A/TN/1-560/2009 (H1N1). (B) A/Bethesda/55/2015 (H3N2). (C) A/Aichi/2/1968 (H3N2). (D) rg-A/swine/NC/18161/2002 (H1N1). (E) A/swine/North Carolina/0033/2011 (H3N2). (F) A/swine/North Carolina/1256/2011 (H3N2). (G) A/swine/California/T9001707/1991 (H1N1). (H) A/canine/Illinois/41915/2015 (H3N2). (I) rg-A/canine/Indiana/1177-17-1/2017 (PR8 internal genes) (H3N2). (J) A/ruddy turnstone/NJ/65/1985 (H7N3). (K) A/duck/Australia/341/1983 (H15N8). (L) A/gull/Maryland/704/1977 (H13N6).

Journal: PLoS Pathogens

Article Title: Relationship between hemagglutinin stability and influenza virus persistence after exposure to low pH or supraphysiological heating

doi: 10.1371/journal.ppat.1009910

Figure Lengend Snippet: TCID50 values (left y-axis, black circles) and luciferase activity (RLU, right y-axis, white squares) were measured as a function of exposure to the reported pH followed by re-neutralization. Right y-axes are scaled so that RLU maxima and minima are displayed at levels similar to those from TCID50 on the opposite axis. Error bars represent standard deviation (n = 3). Reported data are representative of two or more independent experiments. (A) rg-A/TN/1-560/2009 (H1N1). (B) A/Bethesda/55/2015 (H3N2). (C) A/Aichi/2/1968 (H3N2). (D) rg-A/swine/NC/18161/2002 (H1N1). (E) A/swine/North Carolina/0033/2011 (H3N2). (F) A/swine/North Carolina/1256/2011 (H3N2). (G) A/swine/California/T9001707/1991 (H1N1). (H) A/canine/Illinois/41915/2015 (H3N2). (I) rg-A/canine/Indiana/1177-17-1/2017 (PR8 internal genes) (H3N2). (J) A/ruddy turnstone/NJ/65/1985 (H7N3). (K) A/duck/Australia/341/1983 (H15N8). (L) A/gull/Maryland/704/1977 (H13N6).

Article Snippet: 14 , A/Florida/3/2006 (H1N1) , human , 5.30 , 5.20 , , 0.10 , , ATCC VR-1893.

Techniques: Luciferase, Activity Assay, Neutralization, Standard Deviation

The percent of virus inactivation by TCID50 assay was calculated at pH values ranging from 5.3 to 5.9 at 0.1-unit increments. These values are displayed to the right of associated micrographs. HA activation pH values by syncytia assay and inactivation pH values by luciferase assay are provided at the top of each column for reference. The viruses are A/TN/09 WT, A/swine/Wisconsin/11/1980 (H1N1), A/swine/California/T9001707/1991 (H1N1), and A/black-headed gull/Sweden/5/1999 (H16N2).

Journal: PLoS Pathogens

Article Title: Relationship between hemagglutinin stability and influenza virus persistence after exposure to low pH or supraphysiological heating

doi: 10.1371/journal.ppat.1009910

Figure Lengend Snippet: The percent of virus inactivation by TCID50 assay was calculated at pH values ranging from 5.3 to 5.9 at 0.1-unit increments. These values are displayed to the right of associated micrographs. HA activation pH values by syncytia assay and inactivation pH values by luciferase assay are provided at the top of each column for reference. The viruses are A/TN/09 WT, A/swine/Wisconsin/11/1980 (H1N1), A/swine/California/T9001707/1991 (H1N1), and A/black-headed gull/Sweden/5/1999 (H16N2).

Article Snippet: 14 , A/Florida/3/2006 (H1N1) , human , 5.30 , 5.20 , , 0.10 , , ATCC VR-1893.

Techniques: Virus, TCID50 Assay, Activation Assay, Luciferase