32006 Search Results


apodemus spp  (ATCC)
94
ATCC apodemus spp
Reactivity of MAbs with Bartonella antigens
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phosphoric acid  (Chem Impex International)
96
Chem Impex International phosphoric acid
Reactivity of MAbs with Bartonella antigens
Phosphoric Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a solomon islands 3 2006  (Addgene inc)
90
Addgene inc a solomon islands 3 2006
Reactivity of MAbs with Bartonella antigens
A Solomon Islands 3 2006, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lightswitch positive control rpl10 promoter #32006  (Active Motif)
90
Active Motif lightswitch positive control rpl10 promoter #32006
Reactivity of MAbs with Bartonella antigens
Lightswitch Positive Control Rpl10 Promoter #32006, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glucose oxidase-coated strips abbott #32006-6  (Abbott Laboratories)
90
Abbott Laboratories glucose oxidase-coated strips abbott #32006-6
Reactivity of MAbs with Bartonella antigens
Glucose Oxidase Coated Strips Abbott #32006 6, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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negative control lightswitch random promoter #32006  (Active Motif)
90
Active Motif negative control lightswitch random promoter #32006
( A ) A673 ( n = 4) and CHLA-10 ( n = 4) cells were treated with control or EWS-FLI1 siRNA for 48 hours, followed by treatment with 500 U/mL IFN-γ for a subsequent 24 hours. Ewing cells were analyzed by RT-PCR analysis for ICAM-1 and RPLP0 (control) expression. Graphs represent relative increase in ICAM1 mRNA expression (values normalized to untreated control). Bars represent SD. Differences in IFN-γ treatment response between ctsi and EWFsi groups for each cell line was compared using an unpaired t -test. *** p < 0.001. ( B ) Cells were treated with IFN-γ as in (A) followed by analysis of ICAM-1 surface expression by flow cytometry. MFI (median fluorescence intensity) values for both control (ct) and EWS-FLI1 (EWF) siRNA treatment conditions in both the A673 and CHLA10 cell lines are listed. ( C ) shA673-1c cells (harboring shRNA mediated tet-repressible EWS-FLI1 vector) were treated with DMSO vehicle control or 1 microgram/mL doxycycline for 48 hours ( n = 3). RT-PCR analysis of EWS-FLI1 and RPLP0 (control) expression was performed. *** p < 0.001. ( D ) An ICAM1 <t>LightSwitch</t> promoter reporter assay was used to examine differences in promoter activity between shA673-1c cells + DOX (doxycycline) or DMSO control cells in the absence or presence of IFN-γ. In this system, +Dox= EWS-FLI1 ‘low’ expression. Differences in promoter activity between conditions were determined using an unpaired t -test. * p < 0.05.
Negative Control Lightswitch Random Promoter #32006, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell-culture plate 32006  (SPL Life Sciences)
90
SPL Life Sciences cell-culture plate 32006
( A ) A673 ( n = 4) and CHLA-10 ( n = 4) cells were treated with control or EWS-FLI1 siRNA for 48 hours, followed by treatment with 500 U/mL IFN-γ for a subsequent 24 hours. Ewing cells were analyzed by RT-PCR analysis for ICAM-1 and RPLP0 (control) expression. Graphs represent relative increase in ICAM1 mRNA expression (values normalized to untreated control). Bars represent SD. Differences in IFN-γ treatment response between ctsi and EWFsi groups for each cell line was compared using an unpaired t -test. *** p < 0.001. ( B ) Cells were treated with IFN-γ as in (A) followed by analysis of ICAM-1 surface expression by flow cytometry. MFI (median fluorescence intensity) values for both control (ct) and EWS-FLI1 (EWF) siRNA treatment conditions in both the A673 and CHLA10 cell lines are listed. ( C ) shA673-1c cells (harboring shRNA mediated tet-repressible EWS-FLI1 vector) were treated with DMSO vehicle control or 1 microgram/mL doxycycline for 48 hours ( n = 3). RT-PCR analysis of EWS-FLI1 and RPLP0 (control) expression was performed. *** p < 0.001. ( D ) An ICAM1 <t>LightSwitch</t> promoter reporter assay was used to examine differences in promoter activity between shA673-1c cells + DOX (doxycycline) or DMSO control cells in the absence or presence of IFN-γ. In this system, +Dox= EWS-FLI1 ‘low’ expression. Differences in promoter activity between conditions were determined using an unpaired t -test. * p < 0.05.
Cell Culture Plate 32006, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Reactivity of MAbs with Bartonella antigens

Journal:

Article Title: Production of Bartonella Genus-Specific Monoclonal Antibodies

doi: 10.1128/CDLI.8.4.847-849.2001

Figure Lengend Snippet: Reactivity of MAbs with Bartonella antigens

Article Snippet: After being crushed and smeared onto microscope slides the lice were tested for Bartonella by MIF as described above with ascitic fluid of hybridoma B2D3 diluted 1:1,000. table ft1 table-wrap mode="anchored" t5 caption a7 Species Strain Source a Titer of MAb: B2D3 B3D4 B. henselae Houston-1 (ATCC 4988) Bacteremia (27) 6,400 12,800 B. henselae San Ant 2 (SA2) Cat scratch disease (6) 25,600 25,600 B. henselae CAL-1 Septicemia, United States 6,400 3,200 B. henselae URBHLLY8 (CIP 104756) Cat scratch disease (7) 6,400 3,200 B. henselae URBHLIE9 Endocarditis (7) 6,400 6,400 B. quintana URBQMLY15 Chronic lymphadenopathy (9, 21) 6,400 12,800 B. quintana Fuller (ATCC VR-358) Trench fever (30) 6,400 12,800 B. quintana SH-PERM Trench fever, Russia 6,400 12,800 B. clarridgeiae URBCMNHC26 Blood of cat, France 6,400 12,800 B. elizabethae F9251 (ATCC 49927) Endocarditis (5) 6,400 3,200 B. grahamii V2 (NTCC 12860) Blood of Clethrionomys glareolus (2) 6,400 3,200 B. taylorii M6 (NTCC 12861) Blood of Apodemus spp. (2) 3,200 6,400 B. doshiae R18 (NTCC 12862) Blood of Microtus agrestis (2) 6,400 12,800 B. vinsonii subsp. vinsonii Baker (ATCC VR-152) Spleen of Microtus pennsylvanicus (31) 3,200 3,200 B. vinsonii subsp. arupensis OK 94-513 (ATCC 700727) Bacteremia (32) 6,400 12,800 B. vinsonii subsp. berkhoffii NCSV93-CO1 (ATCC 51672) Blood of dog (18) 1,600 3,200 B. alsatica IBS 383 (CIP 105477) Blood of rabbit (13) 6,400 12,800 B. koehlerae C-29 (ATCC 700693) Blood of cat (10) 6,400 12,800 B. tribocorum IBS 506 (CIP 105476) Blood of rat (14) 1,600 6,400 B. bacilliformis Monzon 812 Blood of bartonellosis patient, Peru 3,200 12,800 C. psittaci 50 800 C. trachomatis <25 400 C. pneumoniae <25 <25 23 species b <25 <25 Open in a separate window a Geographic origin is given if the isolation of the strain is not detailed elsewhere. b Includes E. coli, Klebsiella pneumoniae, Enterobacter aerogenes, Yersinia enterocolitica, Shigella dysenteriae, Salmonella enterica, Campylobacter jejuni, Brucella melitensis, Ochrobactrum anthropi, Haemophilus influenzae, Kingella kingae, Neisseria meningitidis, Bacteroides fragilis, Desulfovibrio fairfieldensis, Fusobacterium necrophorum, Enterococcus faecalis, Afipia clevelandensis, Afipia felis, Pseudomonas aeruginosa, Pseudomonas putida, Burkholderia cepacia, Stenotrophomonas maltophilia, and Coxiella burnetii .

Techniques:

( A ) A673 ( n = 4) and CHLA-10 ( n = 4) cells were treated with control or EWS-FLI1 siRNA for 48 hours, followed by treatment with 500 U/mL IFN-γ for a subsequent 24 hours. Ewing cells were analyzed by RT-PCR analysis for ICAM-1 and RPLP0 (control) expression. Graphs represent relative increase in ICAM1 mRNA expression (values normalized to untreated control). Bars represent SD. Differences in IFN-γ treatment response between ctsi and EWFsi groups for each cell line was compared using an unpaired t -test. *** p < 0.001. ( B ) Cells were treated with IFN-γ as in (A) followed by analysis of ICAM-1 surface expression by flow cytometry. MFI (median fluorescence intensity) values for both control (ct) and EWS-FLI1 (EWF) siRNA treatment conditions in both the A673 and CHLA10 cell lines are listed. ( C ) shA673-1c cells (harboring shRNA mediated tet-repressible EWS-FLI1 vector) were treated with DMSO vehicle control or 1 microgram/mL doxycycline for 48 hours ( n = 3). RT-PCR analysis of EWS-FLI1 and RPLP0 (control) expression was performed. *** p < 0.001. ( D ) An ICAM1 LightSwitch promoter reporter assay was used to examine differences in promoter activity between shA673-1c cells + DOX (doxycycline) or DMSO control cells in the absence or presence of IFN-γ. In this system, +Dox= EWS-FLI1 ‘low’ expression. Differences in promoter activity between conditions were determined using an unpaired t -test. * p < 0.05.

Journal: Oncotarget

Article Title: EWS-FLI1 low Ewing sarcoma cells demonstrate decreased susceptibility to T-cell-mediated tumor cell apoptosis

doi: 10.18632/oncotarget.26939

Figure Lengend Snippet: ( A ) A673 ( n = 4) and CHLA-10 ( n = 4) cells were treated with control or EWS-FLI1 siRNA for 48 hours, followed by treatment with 500 U/mL IFN-γ for a subsequent 24 hours. Ewing cells were analyzed by RT-PCR analysis for ICAM-1 and RPLP0 (control) expression. Graphs represent relative increase in ICAM1 mRNA expression (values normalized to untreated control). Bars represent SD. Differences in IFN-γ treatment response between ctsi and EWFsi groups for each cell line was compared using an unpaired t -test. *** p < 0.001. ( B ) Cells were treated with IFN-γ as in (A) followed by analysis of ICAM-1 surface expression by flow cytometry. MFI (median fluorescence intensity) values for both control (ct) and EWS-FLI1 (EWF) siRNA treatment conditions in both the A673 and CHLA10 cell lines are listed. ( C ) shA673-1c cells (harboring shRNA mediated tet-repressible EWS-FLI1 vector) were treated with DMSO vehicle control or 1 microgram/mL doxycycline for 48 hours ( n = 3). RT-PCR analysis of EWS-FLI1 and RPLP0 (control) expression was performed. *** p < 0.001. ( D ) An ICAM1 LightSwitch promoter reporter assay was used to examine differences in promoter activity between shA673-1c cells + DOX (doxycycline) or DMSO control cells in the absence or presence of IFN-γ. In this system, +Dox= EWS-FLI1 ‘low’ expression. Differences in promoter activity between conditions were determined using an unpaired t -test. * p < 0.05.

Article Snippet: The LightSwitch Luciferase Assay Kit (#32031), GoClone human ICAM1 promoter reporter (S708388), LightSwitch positive control RPL10 promoter (#32006) and negative control LightSwitch random promoter (#32006) were purchased from Active Motif (Carlsbad, CA, USA) and used in experiments according to the manufacturer’s instructions.

Techniques: Control, Reverse Transcription Polymerase Chain Reaction, Expressing, Flow Cytometry, Fluorescence, shRNA, Plasmid Preparation, Reporter Assay, Activity Assay