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3156002a  (fluidigm)


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    Structured Review

    fluidigm 3156002a
    3156002a, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3156002a/Anti-p38+%5BT180%2FY182%5D+(D3F9)-156Gd/pmc12645211-60-9-6
    Average 94 stars, based on 13 article reviews
    3156002a - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Marker:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Blocking Assay:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Virus:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Recombinant:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Plasmid Preparation:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Saline:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Lysis:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Reverse Transcription:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    In Vivo:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Electroporation:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Mass Cytometry:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Conjugation Assay:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Illumina Sequencing:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Library Quantification:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    In Vitro:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Quantitative RT-PCR:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Software:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Real-time Polymerase Chain Reaction:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Cytometry:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Microscopy:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Imaging:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Spectrophotometry:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Chemotaxis Assay:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Activity Assay:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Sequencing:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Binding Assay:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Migration:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Western Blot:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.

    Isolation:

    Article Title: Unsupervised machine learning reveals risk stratifying glioblastoma tumor cells
    Article Snippet: Antibody , Anti-p-p38 (T180/Y182) (rabbit-monoclonal) , Fluidigm , RRID: AB_2661826 Cat# 3156002A Clone: D3F9 , MC (1:100).

    Article Title: Inhibition of mitochondrial complex I reverses NOTCH1 -driven metabolic reprogramming in T-cell acute lymphoblastic leukemia
    Article Snippet: 156 Gd , p-p38 (180/182) , D3F9 , DVS-Fluidigm , 3156002A , YES.

    Article Title: AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Article Snippet: Significance (P) neutrophil chemotaxis .28 21.71 3.49E-03 negative regulation of MAP kinase activity .32 21.56 3.81E-04 response to gamma radiation .24 21.11 3.83E-02 regulation of ERK1 and ERK2 cascade 1.13 9.72 1.74E-04 regulation of angiogenesis .99 9.12 6.09E-03 regulation of cysteine-type endopeptidase activity involved in apoptotic process .89 8.98 2.89E-02 aging 1.32 8.33 8.16E-04 response to lipopolysaccharide 1.43 7.67 1.85E-03 positive regulation of response to external stimulus 1.18 7.60 2.68E-02 inflammatory response 2.11 7.57 3.03E-06 response to hypoxia 1.40 7.17 1.24E-02 cellular response to growth factor stimulus 2.07 6.76 1.72E-04 regulation of sequence-specific DNA binding transcription factor activity 1.65 6.67 7.22E-03 blood vessel morphogenesis 1.67 6.58 8.20E-03 morphogenesis of an epithelium 1.84 6.51 2.84E-03 MAPK cascade 1.63 6.14 4.79E-02 positive regulation of cell migration 1.80 6.12 1.66E-02 reproductive structure development 1.86 5.91 2.28E-02 positive regulation of apoptotic process 2.59 5.80 3.83E-04 A B Antibody target Clone Metal tag Conc./Dilution Product number / Supplier Cleaved caspase 3 5A1E (cleaved at Asp175) 142 Nd 1 μg/ml 9661, CST pStat3 4 (pY705) 145 Nd 4 μg/ml 612356, BD pStat5 47 (pY694) 146 Nd 2 μg/ml 61964, BD PD-L1 29E.2A3 148 Nd 1:50 3148017B, Fluidigm pEGFR Y38 (pTyr1068) 149 Sm 0.5 μg/ml ab5644, Abcam pRb J112-906 (pSer807/811) 150 Nd 1:200 201313, Fluidigm pAkt D9E (pS473) 152 Sm 1:50 3152005A, Fluidigm pStat1 4a (pY701) 153 Eu 1:50 3153005A, Fluidigm Vimentin D21H3 154 Sm 4 μg/ml 5741, CST p38 D3F9 156 Gd 1:50 3156002A, Fluidigm E-cadherin 24E11 158 Gd 1:200 3158021A, Fluidigm pMAPKAPK2 27B7 159 Tb 1:50 3158021A, Fluidigm pErk1/2 20A (pT202/pY204) 161 Gd 1 μg/ml 612358, BD pPLCg2 K86-689.37 (pY759) 162 Dy 2 μg/ml 558490, BD TGFβ TW46H10 163 Dy 1:200 3163010B, Fluidigm pNFκB K10-895.12.50 (pS529) 166 Er 0.5 μg/ml 558393, BD YAP H9 (C-terminal 379-407) 167 Er 1 μg/ml sc-271134, Santa Cruz Axl 1H12 (total) 168 Er 2 μg/ml BerGenBio EGFR AY13 170 Er 1:400 3170009B, Fluidigm pS6 N7-548 (pS235/S236) 172 Yb 1:200 3172008A, Fluidigm pHistone H3 HTA28 (pSer28) 175 Lu 1:200 201313, Fluidigm CD44 IM7 (total, surface) 176 Yb 0.125 μg/ml 553131, BD Table S2 Target Product Number Supplier Dilution (Application) Host Species Apoptosis cocktail: actin, caspase 3, PARP ab136812 Abcam 1:250 (WB) Rabbit/ Mouse AXL 1H12-1B7-5D6 BerGenBio 1:1000 (WB) Mouse AXL MAB154 R&D Systems 1:100 (Flow) Mouse Beclin-1 3495 Cell Signaling Tech.

    Article Title: Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 17.5.

    Article Title: High-Dimensional Phospho-CyTOF Characterization of T-Cell Activation Responses in Whole Blood
    Article Snippet: 156 Gd , pP38 [T180/Y182] , Standard BioTools , 3156002A , D3F9 , 18.

    Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
    Article Snippet: Anti-human/mouse pp38 [T180/182] 156Gd clone D3F9 , Fluidigm , 3156002A.

    Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Article Snippet: 156 Gd , p38 [T180/Y182] , D3F9 , Fluidigm 3156002A.



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    Image Search Results


    The 29 antibody panels used to stain PBMCs for CyTOF analysis. PBMCs: Peripheral blood mononuclear cells, CyTOF: Time of flight mass cytometry

    Journal: Cureus

    Article Title: Acute Effects of Growth Hormone on the Cellular Immunologic Landscape in Pediatric Patients

    doi: 10.7759/cureus.57383

    Figure Lengend Snippet: The 29 antibody panels used to stain PBMCs for CyTOF analysis. PBMCs: Peripheral blood mononuclear cells, CyTOF: Time of flight mass cytometry

    Article Snippet: Pp38 , 156Gd , Fluidigm , D3F9 , 3156002A.

    Techniques: Staining

    The 29 antibody panels used to stain PBMCs for CyTOF analysis. PBMCs: Peripheral blood mononuclear cells, CyTOF: Time of flight mass cytometry

    Journal: Cureus

    Article Title: Acute Effects of Growth Hormone on the Cellular Immunologic Landscape in Pediatric Patients

    doi: 10.7759/cureus.57383

    Figure Lengend Snippet: The 29 antibody panels used to stain PBMCs for CyTOF analysis. PBMCs: Peripheral blood mononuclear cells, CyTOF: Time of flight mass cytometry

    Article Snippet: Pp38 , 156Gd , Fluidigm , D3F9 , 3156002A.

    Techniques: Staining

    ( A ) Box-and-whisker plots depicting cytokine release of monocytes (500,000 cells per well, 10 healthy donors) preincubated for 1 hour with either myriocin or PF543, and subsequently stimulated for 24 hours with lipopolysaccharide (LPS; 100 ng/mL). The y axis depicts concentration in pg/mL (±SEM, vertical bars). The colors indicate the different conditions. Significance was determined by a paired Wilcoxon’s rank-sum test. A P value below 0.05 was considered significant. * P < 0.05, ** P < 0.01. ( B ) Identical to panel A , but here for neutrophils (500,000 cells per well, 8 healthy donors), stimulated for 2 hours with LPS. ( C ) Box-and-whisker plots showing levels (in pmol/mL) of ceramide species in monocytes incubated with either vehicle or myriocin, measured by targeted MS ( n = 4 per condition). Cer, ceramide; dh-Cer, dihydroceramide; glcCer, glucosylceramide; lacCer, lactosylceramide; Gb3, globotriaosylceramide. ( D ) Box-and-whisker plots showing levels (in pmol/mL) of S1P in monocytes and neutrophils incubated with either vehicle or PF543, measured by targeted MS ( n = 4 per condition). S1P, sphingosine-1-phosphate. ( E ) Box-and-whisker plots showing intensity levels of intracellular phosphorylation markers p-ERK-171Yb (pERK), p-NF-κB-Er166 (p-NF-κB), and p-p38-Gd156 (pp38) in monocytes and neutrophils after LPS stimulation and treatment with either myriocin or PF543, measured by cytometry by time of flight (CyTOF). The y axis indicates the arsinh-normalized medium intensity levels. There were no significant differences ( n =4 per condition). ( F ) Box-and-whisker plots showing mRNA levels for key cytokines in monocytes treated with vehicle, myriocin, or PF543, measured by qRT-PCR. There were no significant differences ( n = 4 per condition).

    Journal: JCI Insight

    Article Title: The shifting lipidomic landscape of blood monocytes and neutrophils during pneumonia

    doi: 10.1172/jci.insight.164400

    Figure Lengend Snippet: ( A ) Box-and-whisker plots depicting cytokine release of monocytes (500,000 cells per well, 10 healthy donors) preincubated for 1 hour with either myriocin or PF543, and subsequently stimulated for 24 hours with lipopolysaccharide (LPS; 100 ng/mL). The y axis depicts concentration in pg/mL (±SEM, vertical bars). The colors indicate the different conditions. Significance was determined by a paired Wilcoxon’s rank-sum test. A P value below 0.05 was considered significant. * P < 0.05, ** P < 0.01. ( B ) Identical to panel A , but here for neutrophils (500,000 cells per well, 8 healthy donors), stimulated for 2 hours with LPS. ( C ) Box-and-whisker plots showing levels (in pmol/mL) of ceramide species in monocytes incubated with either vehicle or myriocin, measured by targeted MS ( n = 4 per condition). Cer, ceramide; dh-Cer, dihydroceramide; glcCer, glucosylceramide; lacCer, lactosylceramide; Gb3, globotriaosylceramide. ( D ) Box-and-whisker plots showing levels (in pmol/mL) of S1P in monocytes and neutrophils incubated with either vehicle or PF543, measured by targeted MS ( n = 4 per condition). S1P, sphingosine-1-phosphate. ( E ) Box-and-whisker plots showing intensity levels of intracellular phosphorylation markers p-ERK-171Yb (pERK), p-NF-κB-Er166 (p-NF-κB), and p-p38-Gd156 (pp38) in monocytes and neutrophils after LPS stimulation and treatment with either myriocin or PF543, measured by cytometry by time of flight (CyTOF). The y axis indicates the arsinh-normalized medium intensity levels. There were no significant differences ( n =4 per condition). ( F ) Box-and-whisker plots showing mRNA levels for key cytokines in monocytes treated with vehicle, myriocin, or PF543, measured by qRT-PCR. There were no significant differences ( n = 4 per condition).

    Article Snippet: Following this, cells were washed and stained once again for intracellular phosphorylation markers pERK-171Yb, pNfkB-Er166, and pp38-Gd156 (catalog 3171010A, 3166026D, and 3156002A, respectively; Standard Biotools).

    Techniques: Whisker Assay, Concentration Assay, Incubation, Phospho-proteomics, Cytometry, Quantitative RT-PCR