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atcc 29750  (ATCC)


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    ATCC atcc 29750
    Atcc 29750, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atcc 29750/product/ATCC
    Average 91 stars, based on 10 article reviews
    atcc 29750 - by Bioz Stars, 2026-02
    91/100 stars

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    Image Search Results


    Figure 4. Nox4 inhibition by siRNA mitigates Ang II-induced oxidative stress. (A) Nox4 inhibition by siRNA reduces Nox4 protein as expected, and 50 nM of Nox4 siRNA is a sufficient transfection dose. Nox4 siRNA significantly reduces Nox4 protein level (B: n = 3) and the oxidative stress (C: n = 4) increased by Ang II. Data are presented as mean ± standard deviation. *p < 0.05 and **p < 0.01 vs. control and

    Journal: Kidney research and clinical practice

    Article Title: Upregulation of NADH/NADPH oxidase 4 by angiotensin II induces podocyte apoptosis.

    doi: 10.23876/j.krcp.22.198

    Figure Lengend Snippet: Figure 4. Nox4 inhibition by siRNA mitigates Ang II-induced oxidative stress. (A) Nox4 inhibition by siRNA reduces Nox4 protein as expected, and 50 nM of Nox4 siRNA is a sufficient transfection dose. Nox4 siRNA significantly reduces Nox4 protein level (B: n = 3) and the oxidative stress (C: n = 4) increased by Ang II. Data are presented as mean ± standard deviation. *p < 0.05 and **p < 0.01 vs. control and

    Article Snippet: Briefly, Nox4 siRNA (sc-41587; Santa Cruz Biotechnology), AT1R siRNA (sc-29751; Santa Cruz Biotechnology), or control scrambled siRNA (Santa Cruz Biotechnology) were diluted into each six-well plate with transfection medium (Opti-MEM; Invitrogen) and incubated for 5 minutes.

    Techniques: Inhibition, Transfection, Standard Deviation, Control

    Figure 5. Probucol and Nox4 siRNA reduce Ang II-induced podocyte apoptosis. Ang II induces podocyte apoptosis as determined using fluorescence-activated cell sorting assay, and that is improved with probucol (A) and Nox4 siRNA but not by scrambled siRNA (B). Data are expressed as mean ± standard deviation (n = 3). **p < 0.01 vs. control and

    Journal: Kidney research and clinical practice

    Article Title: Upregulation of NADH/NADPH oxidase 4 by angiotensin II induces podocyte apoptosis.

    doi: 10.23876/j.krcp.22.198

    Figure Lengend Snippet: Figure 5. Probucol and Nox4 siRNA reduce Ang II-induced podocyte apoptosis. Ang II induces podocyte apoptosis as determined using fluorescence-activated cell sorting assay, and that is improved with probucol (A) and Nox4 siRNA but not by scrambled siRNA (B). Data are expressed as mean ± standard deviation (n = 3). **p < 0.01 vs. control and

    Article Snippet: Briefly, Nox4 siRNA (sc-41587; Santa Cruz Biotechnology), AT1R siRNA (sc-29751; Santa Cruz Biotechnology), or control scrambled siRNA (Santa Cruz Biotechnology) were diluted into each six-well plate with transfection medium (Opti-MEM; Invitrogen) and incubated for 5 minutes.

    Techniques: Fluorescence, FACS, Standard Deviation, Control

    Figure 6. AT1R siRNA inhibits Ang II-induced oxidative stress and subsequent podocyte apoptosis. (A) AT1R inhibition by siRNA reduces AT1R protein, and 400 nM of AT1R siRNA is a sufficient transfection dose. (B) AT1R siRNA significantly reduces AT1R protein upregulated by Ang II (n = 3). Ang II-induced oxidative stress and podocyte apoptosis are improved by AT1R siRNA (C and D, respective ly; n = 3). Data are presented as mean ± standard deviation. *p < 0.05 and **p < 0.01 vs. control and

    Journal: Kidney research and clinical practice

    Article Title: Upregulation of NADH/NADPH oxidase 4 by angiotensin II induces podocyte apoptosis.

    doi: 10.23876/j.krcp.22.198

    Figure Lengend Snippet: Figure 6. AT1R siRNA inhibits Ang II-induced oxidative stress and subsequent podocyte apoptosis. (A) AT1R inhibition by siRNA reduces AT1R protein, and 400 nM of AT1R siRNA is a sufficient transfection dose. (B) AT1R siRNA significantly reduces AT1R protein upregulated by Ang II (n = 3). Ang II-induced oxidative stress and podocyte apoptosis are improved by AT1R siRNA (C and D, respective ly; n = 3). Data are presented as mean ± standard deviation. *p < 0.05 and **p < 0.01 vs. control and

    Article Snippet: Briefly, Nox4 siRNA (sc-41587; Santa Cruz Biotechnology), AT1R siRNA (sc-29751; Santa Cruz Biotechnology), or control scrambled siRNA (Santa Cruz Biotechnology) were diluted into each six-well plate with transfection medium (Opti-MEM; Invitrogen) and incubated for 5 minutes.

    Techniques: Inhibition, Transfection, Standard Deviation, Control

    Figure 7. Schematic view of oxidative stress and apoptosis induced by Ang II in a podocyte. Ang II induces podocyte oxidative stress and subsequent apoptosis with AT1R and via upregulation of Nox4. Ang, angiotensin; AT1R, angiotensin II type 1 receptor; Nox4, NADH/NADPH oxidase 4.

    Journal: Kidney research and clinical practice

    Article Title: Upregulation of NADH/NADPH oxidase 4 by angiotensin II induces podocyte apoptosis.

    doi: 10.23876/j.krcp.22.198

    Figure Lengend Snippet: Figure 7. Schematic view of oxidative stress and apoptosis induced by Ang II in a podocyte. Ang II induces podocyte oxidative stress and subsequent apoptosis with AT1R and via upregulation of Nox4. Ang, angiotensin; AT1R, angiotensin II type 1 receptor; Nox4, NADH/NADPH oxidase 4.

    Article Snippet: Briefly, Nox4 siRNA (sc-41587; Santa Cruz Biotechnology), AT1R siRNA (sc-29751; Santa Cruz Biotechnology), or control scrambled siRNA (Santa Cruz Biotechnology) were diluted into each six-well plate with transfection medium (Opti-MEM; Invitrogen) and incubated for 5 minutes.

    Techniques:

    The expression of IL-6 and CD73 in NPC. a. Representative images for the IHC staining of IL-6 and CD73 in NPC and normal tissues. b. The relative expression levels of IL-6 and CD73 were analyzed by pathological score (PS) in all tissues. c The expression of IL-6 and CD73 in NPC and normal tissues were analyzed by HNSC RNA expression profile datasets from TCGA. d-e The differences in IL-6 and CD73 expression in different stages of NPC sections were analyzed based on PS (d) and TCGA datasets (e) . f-g Results from the Spearman correlation analysis of IL-6 with CD73 in all tissues based on PS (f) and TCGA datasets (g) . *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Journal: Journal of Cancer

    Article Title: Mesenchymal stem/stromal cells-derived IL-6 promotes nasopharyngeal carcinoma growth and resistance to cisplatin via upregulating CD73 expression

    doi: 10.7150/jca.37932

    Figure Lengend Snippet: The expression of IL-6 and CD73 in NPC. a. Representative images for the IHC staining of IL-6 and CD73 in NPC and normal tissues. b. The relative expression levels of IL-6 and CD73 were analyzed by pathological score (PS) in all tissues. c The expression of IL-6 and CD73 in NPC and normal tissues were analyzed by HNSC RNA expression profile datasets from TCGA. d-e The differences in IL-6 and CD73 expression in different stages of NPC sections were analyzed based on PS (d) and TCGA datasets (e) . f-g Results from the Spearman correlation analysis of IL-6 with CD73 in all tissues based on PS (f) and TCGA datasets (g) . *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Article Snippet: For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).

    Techniques: Expressing, Immunohistochemistry, RNA Expression

    Comparison of gp80, gp130, p-STAT3, MMP-9, α-SMA, Ki-67, SOX-2, and vimentin expression and prognosis between NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype. a. Representative images for the IHC staining of gp80, gp130, p-STAT3, MMP-9, α-SMA, Ki-67, SOX-2, and vimentin in NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype. b - i. Bar graphic figures showing the relative expression levels, basing on pathological score (PS) of gp80 ( b ), gp130 ( c ), p-STAT3 ( d ), MMP-9 ( e ), α-SMA ( f ), Ki-67 ( g ), SOX-2 ( h ), and Vimentin ( i ) in NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype. j - k. Comparison of prognosis between NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype basing on PS ( j ) and TCGA datasets ( k ). *, P < 0.05; **, P < 0.01.

    Journal: Journal of Cancer

    Article Title: Mesenchymal stem/stromal cells-derived IL-6 promotes nasopharyngeal carcinoma growth and resistance to cisplatin via upregulating CD73 expression

    doi: 10.7150/jca.37932

    Figure Lengend Snippet: Comparison of gp80, gp130, p-STAT3, MMP-9, α-SMA, Ki-67, SOX-2, and vimentin expression and prognosis between NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype. a. Representative images for the IHC staining of gp80, gp130, p-STAT3, MMP-9, α-SMA, Ki-67, SOX-2, and vimentin in NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype. b - i. Bar graphic figures showing the relative expression levels, basing on pathological score (PS) of gp80 ( b ), gp130 ( c ), p-STAT3 ( d ), MMP-9 ( e ), α-SMA ( f ), Ki-67 ( g ), SOX-2 ( h ), and Vimentin ( i ) in NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype. j - k. Comparison of prognosis between NPC patients with IL-6 high CD73 high phenotype and IL-6 low CD73 low phenotype basing on PS ( j ) and TCGA datasets ( k ). *, P < 0.05; **, P < 0.01.

    Article Snippet: For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).

    Techniques: Expressing, Immunohistochemistry

    MSC-derived IL-6 induces CD73 expression, activates STAT3 signaling pathway and promotes tumor growth. a. The expression of CD73, IL-6, gp130, gp80, STAT3, p-STAT3, PCNA, Bax, Bcl-2, MMP-9, Vimentin, SOX-2, α-SMA were detected by western blot analysis. Luciferase activity imaging of tumor-bearing mice ( b ). Mice were euthanized and tumor volumes and weight were measured ( c ). *, P < 0.05; P =0.0675 (tumor volume: MSC IL6KO vs MSC); P =0.0960 (tumor weight: MSC IL6KO vs MSC)

    Journal: Journal of Cancer

    Article Title: Mesenchymal stem/stromal cells-derived IL-6 promotes nasopharyngeal carcinoma growth and resistance to cisplatin via upregulating CD73 expression

    doi: 10.7150/jca.37932

    Figure Lengend Snippet: MSC-derived IL-6 induces CD73 expression, activates STAT3 signaling pathway and promotes tumor growth. a. The expression of CD73, IL-6, gp130, gp80, STAT3, p-STAT3, PCNA, Bax, Bcl-2, MMP-9, Vimentin, SOX-2, α-SMA were detected by western blot analysis. Luciferase activity imaging of tumor-bearing mice ( b ). Mice were euthanized and tumor volumes and weight were measured ( c ). *, P < 0.05; P =0.0675 (tumor volume: MSC IL6KO vs MSC); P =0.0960 (tumor weight: MSC IL6KO vs MSC)

    Article Snippet: For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).

    Techniques: Derivative Assay, Expressing, Western Blot, Luciferase, Activity Assay, Imaging

    MSC-derived IL-6 promotes NPC cells resistance to cisplatin via inducing CD73 expression. a. Cell viability of the indicated cells under treatment of MSC IL6KO or MSC-conditioned medium and 20 µM DDP. b. Cell viability of the indicated cells under treatment of 10 ng/mL IL-6 and 20 µM DDP. c. Annexin V-FITC/PI staining of the indicated cells under treatment of 10 ng/mL IL-6 and 20 µM DDP. d. Western blotting analysis of CD73, Vimentin, Bax, Bcl-2, STAT3, p-STAT3 in the indicated cells under treatment of 10 ng/mL IL-6 and 20 µM DDP. *, P < 0.05; **, P < 0.01.

    Journal: Journal of Cancer

    Article Title: Mesenchymal stem/stromal cells-derived IL-6 promotes nasopharyngeal carcinoma growth and resistance to cisplatin via upregulating CD73 expression

    doi: 10.7150/jca.37932

    Figure Lengend Snippet: MSC-derived IL-6 promotes NPC cells resistance to cisplatin via inducing CD73 expression. a. Cell viability of the indicated cells under treatment of MSC IL6KO or MSC-conditioned medium and 20 µM DDP. b. Cell viability of the indicated cells under treatment of 10 ng/mL IL-6 and 20 µM DDP. c. Annexin V-FITC/PI staining of the indicated cells under treatment of 10 ng/mL IL-6 and 20 µM DDP. d. Western blotting analysis of CD73, Vimentin, Bax, Bcl-2, STAT3, p-STAT3 in the indicated cells under treatment of 10 ng/mL IL-6 and 20 µM DDP. *, P < 0.05; **, P < 0.01.

    Article Snippet: For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).

    Techniques: Derivative Assay, Expressing, Staining, Western Blot

    STAT3 transcriptionally activating CD73. a. The putative binding sites of STAT3 in NT5E (encoding CD73 protein) promoters by JASPAR ( http://jaspar.genereg.net/ ). b. Schematic representation of the promoter regions of NT5E with the putative STAT3 binding sites through UCSC table browser ( https://genome.ucsc.edu/cgi-bin/hgTables ). c. Analysis of NT5E promoters physically associated with STAT3 by using ChIP assay in the indicated CNE-2 cells. d Relative luciferase activity of the indicated promoter vectors in the indicated CNE-2 cells.

    Journal: Journal of Cancer

    Article Title: Mesenchymal stem/stromal cells-derived IL-6 promotes nasopharyngeal carcinoma growth and resistance to cisplatin via upregulating CD73 expression

    doi: 10.7150/jca.37932

    Figure Lengend Snippet: STAT3 transcriptionally activating CD73. a. The putative binding sites of STAT3 in NT5E (encoding CD73 protein) promoters by JASPAR ( http://jaspar.genereg.net/ ). b. Schematic representation of the promoter regions of NT5E with the putative STAT3 binding sites through UCSC table browser ( https://genome.ucsc.edu/cgi-bin/hgTables ). c. Analysis of NT5E promoters physically associated with STAT3 by using ChIP assay in the indicated CNE-2 cells. d Relative luciferase activity of the indicated promoter vectors in the indicated CNE-2 cells.

    Article Snippet: For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).

    Techniques: Binding Assay, Luciferase, Activity Assay

    Hypothetical model illustrating that MSC-derived IL-6 upregulates CD73, promotes tumor growth and chemoresistance in NPC.

    Journal: Journal of Cancer

    Article Title: Mesenchymal stem/stromal cells-derived IL-6 promotes nasopharyngeal carcinoma growth and resistance to cisplatin via upregulating CD73 expression

    doi: 10.7150/jca.37932

    Figure Lengend Snippet: Hypothetical model illustrating that MSC-derived IL-6 upregulates CD73, promotes tumor growth and chemoresistance in NPC.

    Article Snippet: For immunostaining, primary antibodies against IL-6 (sc-28343, Santa Cruz), CD73 (PA5-29750, Thermo-Fisher), gp80 (IL-6Rα, sc-373708, Santa Cruz), gp130 (sc-655, Santa Cruz), p-STAT3 (sc-8001-R, Santa Cruz), SOX-2 (66411-1-Ig, Proteintech), MMP-9 (sc-393859, Santa Cruz), Ki-67 (27309-1-AP, Proteintech), Vimentin (sc-373717, Santa Cruz) and α-SMA (55135-1-AP, Proteintech) were incubated for 30 min at room temperature, followed by HRP-conjugated secondary detection antibody and diaminobenzidine (DAB).

    Techniques: Derivative Assay