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nm 011337 2 hsa mir 3653  (ATCC)


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    ATCC nm 011337 2 hsa mir 3653
    Nm 011337 2 Hsa Mir 3653, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/28860/Fusarium+oxysporum+f%2E+sp%2E+luffae+Kawai+et+al/10__1016_slash_j__isci__2024__111388-422-231-265
    Average 90 stars, based on 4 article reviews
    nm 011337 2 hsa mir 3653 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Virus:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Clinical Proteomics:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Recombinant:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Isolation:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Knock-Out:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Quantitative RT-PCR:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Software:

    Article Title: Blocking CCL3-mediated neutrophil recruitment into the brain alleviates immunopathology following severe enterovirus 71 infection
    Article Snippet: .. 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 Jo urn al Pr e-p roo f 27 621 622 STAR METHODS 623 KEY RESOURCES TABLE 624 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Ly6G MAb, 1A8 BioLegend Cat#orb1672083 anti-CCL3 MAb R&D Systems Inc. Cat# MAB66252 anti-CCL2 MAb R&D Systems Inc. Cat# #123616 anti-CD19 BD Pharmingen of BD Biosciences Cat# #555412 anti-CD3 BD Pharmingen of BD Biosciences Cat# #570821 anti-CD4 BD Pharmingen of BD Biosciences Cat# #556615 anti-CD8 BD Pharmingen of BD Biosciences Cat# #555367 Bacterial and virus strains EV71 strain TS Zhao,Z.P et al. GenBank No. JQ708210.1 Biological samples the peripheral blood and the CSF of EV71infected patients Ditan Hospital and Fengtai Maternal & Child Health Care Hospital licensing number: Jing You Ke Lun[2018]045 Chemicals, peptides, and recombinant proteins N-formyl-methionyl-leucyl-phenylalanine Sigma-Aldrich Cat#F3506 Critical commercial assays TRIzol Reagent Invitrogen Cat#15596018CN Neutrophil Separation Kit Hao Yang Biological Manufacture Co., Ltd Cat#LZS11131 murine CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0449 murine CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0568 human CCL3 ELISA kit Boster Biological Engineering Co.,Ltd EK0448 human CCL2 ELISA kit Boster Biological Engineering Co.,Ltd EK0441 Bio-Plex Mouse Cytokine Group I 23-Plex Bio-Rad M60009RDPD Bio-Plex Human Cytokine Group I 27-Plex Bio-Rad M500KCAF0Y mirVana miRNA isolation kit ThermoFisher Scientific AM1561 Deposited data CCL-3 in human NCBI GenBank No. NM_002983.2 CCL-3 in C57BL/6 mouse NCBI GenBank No. NM_011337.2 Hsa-mir-3653 https://www.mirbase.org/ Accession:MI0016053 Hsa-mir-Chr12-2974 NCBI ID number:2886035 Mmu-mir-chr7-17772 NCBI ID number:2886035 Mmu-mir-chr19-40377 NCBI ID number:2886035 Mmu-mir-chr18-39664 NCBI ID number:2886035 Experimental models: Cell lines Jo urn al Pr e-p roo f 28 293T cells ATCC CL-0005 Experimental models: Organisms/strains CCL3 knock-out (KO) mice The Jackson Laboratory Stock No. 002687 CCL2 knock-out (KO) mice The Jackson Laboratory Stock No. 004434 Oligonucleotides qRT-PCR primers Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 program GraphPad Software, Inc. https:www.graphpad.c om/scientificsoftware/prism CellQuestTM analysis software BD Biosciences https://www.bdbioscien ces.com/ EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS 625 Ethics statement 626 All the animal experiments performed in this study were approved and licensed by the Medical 627 Ethics Branch Committee of the Supervisory Committee of Science and Technology of the 628 Academy of Military Medical Science (AMMS), Beijing, China (Licensing number: IACUC 629 of AMMS-2009-02). ..

    Binding Assay:

    Article Title: Complete nucleotide sequences of plasmids pACK2 and pACK5 from Staphylococcus simulans biovar staphylolyticus.
    Article Snippet: Article history: Received 11 August 2012 Accepted 24 January 2013 Available online 6 February 2013 Communicated by Dr. Neville Firth

    Article Title: The genomes and comparative genomics of Lactobacillus delbrueckii phages.
    Article Snippet: Lactobacillus delbrueckii phages are a great source of genetic diversity.. Here, the genome sequences of Lb. delbrueckii phages LL-Ku, c5 and JCL1032 were analyzed in detail, and the genetic diversity of Lb. delbrueckii phages belonging to different taxonomic groups was explored.. The lytic isometric group b phages LL-Ku (31,080 bp) and c5 (31,841 bp) showed a minimum nucleotide sequence identity of 90% over about threefourths of their genomes.

    Mutagenesis:

    Article Title: Complete nucleotide sequences of plasmids pACK2 and pACK5 from Staphylococcus simulans biovar staphylolyticus.
    Article Snippet: Article history: Received 11 August 2012 Accepted 24 January 2013 Available online 6 February 2013 Communicated by Dr. Neville Firth



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    ( A ) Intersection between downregulated genes after stable overexpression of miR-210 in T20 SCs (white circle) and genes with at least one binding site for miR- 210- 3p in their 3′UTR (grey circle). Differentially expressed genes were determined by microarray experiments with T20 SCs after lentiviral transduction of either miR-210 or respective control vectors; significance cutoff was set at FDR < 0.05. Binding site information was retrieved from TargetScan ( www.targetscan.org ). ( B ) Relative expression of miR-210-3p and <t>ISCU</t> for SCs derived from primary tumors T6, T18 and T20, after 72 h of normoxic or hypoxic culture conditions, respectively. Data presented as mean ± SEM of three independent experiments. ( C ) Relative ISCU expression after lentiviral transduction of miR-210, after 72 h normoxia or hypoxia, respectively. Representative figure of 3 independent experiments; data are presented as mean ± SD. ( D ) ISCU protein expression in T20 cells after overexpression of miR-210 (lane 2) or shRNA-mediated knockdown of ISCU (lane 4), compared to respective controls (lanes 1 and 3); representative picture of 3 independent experiments. ( E ) Self-renewal capacity, determined with limiting dilution assays, after stable knockdown of ISCU in T6- and T20-derived SCs. Representative figure of 3 independent experiments; data presented as mean with 95% confidence interval. Statistical significance was assessed with unpaired Student's t -tests for (B) and (C) and with a Chi-square test for (E); * p < 0.05, ** p < 0.01, and *** p < 0.001; N–normoxia, H–hypoxia, shRNA–short hairpin RNA.
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    Image Search Results


    ( A ) Intersection between downregulated genes after stable overexpression of miR-210 in T20 SCs (white circle) and genes with at least one binding site for miR- 210- 3p in their 3′UTR (grey circle). Differentially expressed genes were determined by microarray experiments with T20 SCs after lentiviral transduction of either miR-210 or respective control vectors; significance cutoff was set at FDR < 0.05. Binding site information was retrieved from TargetScan ( www.targetscan.org ). ( B ) Relative expression of miR-210-3p and ISCU for SCs derived from primary tumors T6, T18 and T20, after 72 h of normoxic or hypoxic culture conditions, respectively. Data presented as mean ± SEM of three independent experiments. ( C ) Relative ISCU expression after lentiviral transduction of miR-210, after 72 h normoxia or hypoxia, respectively. Representative figure of 3 independent experiments; data are presented as mean ± SD. ( D ) ISCU protein expression in T20 cells after overexpression of miR-210 (lane 2) or shRNA-mediated knockdown of ISCU (lane 4), compared to respective controls (lanes 1 and 3); representative picture of 3 independent experiments. ( E ) Self-renewal capacity, determined with limiting dilution assays, after stable knockdown of ISCU in T6- and T20-derived SCs. Representative figure of 3 independent experiments; data presented as mean with 95% confidence interval. Statistical significance was assessed with unpaired Student's t -tests for (B) and (C) and with a Chi-square test for (E); * p < 0.05, ** p < 0.01, and *** p < 0.001; N–normoxia, H–hypoxia, shRNA–short hairpin RNA.

    Journal: Oncotarget

    Article Title: Hypoxia-responsive miR-210 promotes self-renewal capacity of colon tumor-initiating cells by repressing ISCU and by inducing lactate production

    doi: 10.18632/oncotarget.11772

    Figure Lengend Snippet: ( A ) Intersection between downregulated genes after stable overexpression of miR-210 in T20 SCs (white circle) and genes with at least one binding site for miR- 210- 3p in their 3′UTR (grey circle). Differentially expressed genes were determined by microarray experiments with T20 SCs after lentiviral transduction of either miR-210 or respective control vectors; significance cutoff was set at FDR < 0.05. Binding site information was retrieved from TargetScan ( www.targetscan.org ). ( B ) Relative expression of miR-210-3p and ISCU for SCs derived from primary tumors T6, T18 and T20, after 72 h of normoxic or hypoxic culture conditions, respectively. Data presented as mean ± SEM of three independent experiments. ( C ) Relative ISCU expression after lentiviral transduction of miR-210, after 72 h normoxia or hypoxia, respectively. Representative figure of 3 independent experiments; data are presented as mean ± SD. ( D ) ISCU protein expression in T20 cells after overexpression of miR-210 (lane 2) or shRNA-mediated knockdown of ISCU (lane 4), compared to respective controls (lanes 1 and 3); representative picture of 3 independent experiments. ( E ) Self-renewal capacity, determined with limiting dilution assays, after stable knockdown of ISCU in T6- and T20-derived SCs. Representative figure of 3 independent experiments; data presented as mean with 95% confidence interval. Statistical significance was assessed with unpaired Student's t -tests for (B) and (C) and with a Chi-square test for (E); * p < 0.05, ** p < 0.01, and *** p < 0.001; N–normoxia, H–hypoxia, shRNA–short hairpin RNA.

    Article Snippet: Primary antibodies against ISCU 1/2 (SC- 28860) and actin (MAB1501) were purchased from Santa Cruz or Merck Millipore, respectively.

    Techniques: Over Expression, Binding Assay, Microarray, Transduction, Control, Expressing, Derivative Assay, shRNA, Knockdown

    ( A ) miR-210-3p and ISCU expression in 47 CRC tumor tissue samples relative to their matching normal counterparts; data are depicted as shaded dots overlaid with their respective distribution as violin plots, with means highlighted in black. ( B ) miR-210-3p expression in paired CRC and matching normal colon tissue samples of publicly available dataset GSE49246. Log 2 expression intensities; data presented as mean ± SD. ( C ) ISCU expression in CRC and normal colon tissue samples in publicly available dataset GSE23878. Log 2 expression intensities; data presented as mean ± SD. Kaplan-Meier curve showing overall ( D ) and relapse-free ( E ) CRC patient survival according to their ISCU expression level. Classification into “high” and “low” expression groups (with 95% confidence intervals) separated using their respective median ISCU expression value. Paired Student's t -test for (A) and (B), unpaired Student's t -test for (C), Cox proportional hazard model for (D) and (E); * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: Oncotarget

    Article Title: Hypoxia-responsive miR-210 promotes self-renewal capacity of colon tumor-initiating cells by repressing ISCU and by inducing lactate production

    doi: 10.18632/oncotarget.11772

    Figure Lengend Snippet: ( A ) miR-210-3p and ISCU expression in 47 CRC tumor tissue samples relative to their matching normal counterparts; data are depicted as shaded dots overlaid with their respective distribution as violin plots, with means highlighted in black. ( B ) miR-210-3p expression in paired CRC and matching normal colon tissue samples of publicly available dataset GSE49246. Log 2 expression intensities; data presented as mean ± SD. ( C ) ISCU expression in CRC and normal colon tissue samples in publicly available dataset GSE23878. Log 2 expression intensities; data presented as mean ± SD. Kaplan-Meier curve showing overall ( D ) and relapse-free ( E ) CRC patient survival according to their ISCU expression level. Classification into “high” and “low” expression groups (with 95% confidence intervals) separated using their respective median ISCU expression value. Paired Student's t -test for (A) and (B), unpaired Student's t -test for (C), Cox proportional hazard model for (D) and (E); * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: Primary antibodies against ISCU 1/2 (SC- 28860) and actin (MAB1501) were purchased from Santa Cruz or Merck Millipore, respectively.

    Techniques: Expressing

    ( A ) Intracellular L-lactate levels after stable overexpression of miR-210 or knockdown of ISCU in T20-derived SCs. Representative figure of 2 independent experiments; data are presented as mean ± SD. ( B ) Ratio of produced lactate per consumed amount of glucose over 72 h for T20 miR-210-overexpressing and respective control cells. Representative experiment of 5 biological replicates, data presented as mean ± SD. ( C ) Schematic representation of central carbon metabolism. U- 13 C gln enters the TCA cycle as 5-times labeled (M5) α-ketoglutarate. One round in the TCA cycle leads to the generation of the M3 α-ketoglutarate isotopologue. ( D ) Ratio of M3 and M5 α-ketoglutarate isotopologues for miR-210-overexpressing T20 and corresponding control cells. Representative figure of 2 independent experiments; data presented as mean ± SD. ( E ) Sphere-forming capacity of T6 and T20 SCs, determined with 1000 cell assays, after stimulation with 2.5 mM L-lactate. Representative figure of 3 independent experiments; data presented as mean ± SD. ( F ) Sphere formation of T20 SCs after inhibition of LDHA with 10 μM NHI-2. Representative figure of 3 independent 1000 cell assays; data presented as mean ± SD. Statistical significance was assessed with unpaired Student's t -tests for (A), (B), (D), (E) and (F); ns – not significant, * p < 0.05, ** p < 0.01, and *** p < 0.001; N–normoxia, H–hypoxia, Lac–lactate.

    Journal: Oncotarget

    Article Title: Hypoxia-responsive miR-210 promotes self-renewal capacity of colon tumor-initiating cells by repressing ISCU and by inducing lactate production

    doi: 10.18632/oncotarget.11772

    Figure Lengend Snippet: ( A ) Intracellular L-lactate levels after stable overexpression of miR-210 or knockdown of ISCU in T20-derived SCs. Representative figure of 2 independent experiments; data are presented as mean ± SD. ( B ) Ratio of produced lactate per consumed amount of glucose over 72 h for T20 miR-210-overexpressing and respective control cells. Representative experiment of 5 biological replicates, data presented as mean ± SD. ( C ) Schematic representation of central carbon metabolism. U- 13 C gln enters the TCA cycle as 5-times labeled (M5) α-ketoglutarate. One round in the TCA cycle leads to the generation of the M3 α-ketoglutarate isotopologue. ( D ) Ratio of M3 and M5 α-ketoglutarate isotopologues for miR-210-overexpressing T20 and corresponding control cells. Representative figure of 2 independent experiments; data presented as mean ± SD. ( E ) Sphere-forming capacity of T6 and T20 SCs, determined with 1000 cell assays, after stimulation with 2.5 mM L-lactate. Representative figure of 3 independent experiments; data presented as mean ± SD. ( F ) Sphere formation of T20 SCs after inhibition of LDHA with 10 μM NHI-2. Representative figure of 3 independent 1000 cell assays; data presented as mean ± SD. Statistical significance was assessed with unpaired Student's t -tests for (A), (B), (D), (E) and (F); ns – not significant, * p < 0.05, ** p < 0.01, and *** p < 0.001; N–normoxia, H–hypoxia, Lac–lactate.

    Article Snippet: Primary antibodies against ISCU 1/2 (SC- 28860) and actin (MAB1501) were purchased from Santa Cruz or Merck Millipore, respectively.

    Techniques: Over Expression, Knockdown, Derivative Assay, Produced, Control, Labeling, Inhibition

    ( A ) Under normoxic conditions, ISCU and oxidative phosphorylation remain unaffected and TICs display a certain degree of self-renewal potential. ( B ) Hypoxia upregulates miR-210, which in turn represses ISCU, thereby leading to an altered TCA cycle activity. The resulting high L-lactate levels suggest that TICs display increased miR-210-induced glycolytic activity under hypoxic conditions, which might provide proliferation benefits and lead to enhanced self-renewal potential of colon TICs.

    Journal: Oncotarget

    Article Title: Hypoxia-responsive miR-210 promotes self-renewal capacity of colon tumor-initiating cells by repressing ISCU and by inducing lactate production

    doi: 10.18632/oncotarget.11772

    Figure Lengend Snippet: ( A ) Under normoxic conditions, ISCU and oxidative phosphorylation remain unaffected and TICs display a certain degree of self-renewal potential. ( B ) Hypoxia upregulates miR-210, which in turn represses ISCU, thereby leading to an altered TCA cycle activity. The resulting high L-lactate levels suggest that TICs display increased miR-210-induced glycolytic activity under hypoxic conditions, which might provide proliferation benefits and lead to enhanced self-renewal potential of colon TICs.

    Article Snippet: Primary antibodies against ISCU 1/2 (SC- 28860) and actin (MAB1501) were purchased from Santa Cruz or Merck Millipore, respectively.

    Techniques: Phospho-proteomics, Activity Assay