bacteriophage vii (ATCC)
Structured Review

Bacteriophage Vii, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bacteriophage vii/product/ATCC
Average 93 stars, based on 5 article reviews
Images
1) Product Images from "Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses"
Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.1714812115
Figure Legend Snippet: Genomic maps of the regions surrounding the 5-HMUDK gene of bacteriophages listed in . GenBank accession numbers as well as the name and host of the bacteriophages are indicated. Notably, 5-HMUDK coassociates with dUMP hydroxymethyltransferase (dU hmt), a member of the thymidylate synthase superfamily.
Techniques Used:
Figure Legend Snippet: Restriction digests of modified and unmodified bacteriophage genomic DNAs. Genomic DNA extracted from the indicated bacteriophages was incubated with restriction enzymes AccI, EcoRI, HinfI, and NdeI. The predicted number of cut sites in each bacteriophage sequence is shown in parentheses next to the given enzyme; λ contains canonical DNA bases only. Phage SP8 DNA contains 5-hmdU, replacing thymidine. Phages SP10 and ΦW-14 contain hypermodified thymidines.
Techniques Used: Modification, Incubation, Sequencing
Figure Legend Snippet: HPLC traces and MS analysis of bacteriophage M6 and ViI nucleosides. The trace in Top was obtained from bacteriophage λ to show the retention of canonical nucleosides. M6 and ViI show a fifth major peak corresponding to the hypermodified base. The protonated molecular ion detected for each hypermodified base is indicated as well as a hypothetical combination of atoms to account for the observed masses. dA, 2′-deoxyadenosine; dG, 2′-deoxyguanosine; dC, 2′-deoxycytidine; dT, thymidine.
Techniques Used:
Figure Legend Snippet: Proposed structures of phage M6 ( A ) and ViI ( B ) modifications. 5- N edU and 5- N e O mdU are shown to be the actual modifications in this work.
Techniques Used: