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antibody labelling kits  (fluidigm)


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    fluidigm antibody labelling kits
    Antibody Labelling Kits, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/201112a/Maxpar+MCP9+Antibody+Labeling+Kit/pmc12322070-32-14-12
    Average 93 stars, based on 5 article reviews
    antibody labelling kits - by Bioz Stars, 2026-10
    93/100 stars

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    Purification:

    Article Title: Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CX3CR1 (8E10.D9), Purified Biolegend Cat# 824001; RRID: AB_2564876 Anti-human HLA-DR (L243), Purified Biolegend Cat# 307602; RRID: AB_314680 Anti-human IgD (IA6-2), Purified Biolegend Cat# 348202; RRID: AB_10550095 Anti-human Siglec-8 (837535), Purified R&D Systems Cat# MAB7975; RRID: N/A Anti-human TCRgd (5A6.E9), Purified Fischer Scientific Cat# TCR1061; RRID: AB_223500 Biological Samples Patients with COVID-19 from Helsinki Hospital District of Helsinki and Uusimaa, Finland N/A Chemicals, Peptides, and Recombinant Proteins Bovine Serum Albumin Sigma-Aldrich Cat# A3059; RRID: N/A Cell-ID Cisplatin Pt194 Fluidigm Cat# 201194; RRID: N/A Cell-ID Cisplatin Pt198 Fluidigm Cat# 201198; RRID: N/A Cisplatin Pt195 BuyIsotope Customized Cisplatin Pt196 BuyIsotope Customized Cell-ID Intercalator-Ir Fluidigm Cat# 201192B; RRID: N/A Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat# 201060; RRID: N/A DMSO Sigma-Aldrich Cat# D8418; RRID: N/A EDTA Rockland Cat# MB-014; RRID: N/A EQ Four Element Calibration Beads Fluidigm Cat# 201078; RRID: N/A FBS Sigma-Aldrich Cat# 12103C; RRID: N/A Fc Receptor (FcR) blocking buffer Cytodelics Customized Maxpar Cell Acquisition Solution (CAS) Fluidigm Cat# 201240; RRID; N/A Maxpar MCP9 Antibody Labeling Kit 110Cd Fluidigm Cat# 201110A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 111Cd Fluidigm Cat# 201111A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 112Cd Fluidigm Cat# 201112A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 113Cd Fluidigm Cat# 201113A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 114Cd Fluidigm Cat# 201114A; RRID: N/A Maxpar Water Fluidigm Cat# 201069; RRID; N/A Maxpar X8 Multimetal Labeling Kit (40 rxn) Fluidigm Cat# 201300; RRID; N/A Metal isotopes as chloride salts (In-115, Gd-155, Gd-157, Dy-161, Dy-163, Yb-173) Trace Sciences International Customized Paraformaldehyde VWR Cat# 16005; RRID: N/A Penicillin-streptomycin Sigma-Aldrich Cat# P4333; RRID: N/A Protein Stabilizer PBS Candor Bioscience Cat# 131125, RRID: N/A PBS 1X Rockland Cat# MB-008; RRID: N/A RPMI 1640 medium Sigma-Aldrich Cat# R848; RRID: N/A Sodium Azide Sigma-Aldrich Cat# 71289; RRID: N/A Whole blood (human) processing kit Cytodelics Cat# hC001-500; RRID: N/A Critical Commercial Assays Immune Response panel Olink AB N/A Inflammation panel Olink AB N/A Other BenchBot robot Agilent technologies Customized Bravo liquid handling platform Agilent technologies Customized (Continued on next page) e2 Cell Reports Medicine 1, 100078, August 25, 2020 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CyTOF 2 upgraded Helios mass cytometer Fluidigm N/A EL406 Washer Dispenser BioTek Customized Helios mass cytometer Fluidigm N/A pluriStrainer Mini, 40 mm pluriSelect Cat# 43-10040-70; RRID: N/A Polypropylene tubes Sarstedt Cat# 55526; RRID: N/A TC20 automated cell counter BioRad N/A Vspin microplate centrifuge Agilent technologies Customized Deposited Data FCS files, Mass cytometry This paper https://brodinlab.com/data-repository/ Protein expression data This paper https://brodinlab.com/data-repository/ IgG data This paper Table S1 Software and Algorithms CyTOF software (v. 6.5.358) N/A https://www.fluidigm.com/ Mass Cytometry Normalizer Finck et al., 201347 https://github.com/nolanlab/ bead-normalization/releases R 3.6.0 R Core Team, 2019 https://www.r-project.org/ Python 3.7.0 https://www.python.org/ Mass Cytometry Debarcoder Zunder et al., 201548 https://github.com/nolanlab/ single-cell-debarcoder CellGrid v0.5.5 Chen et al.13 https://github.com/Brodinlab/cellgrid MOFA Argelaguet et al.27 https://github.com/bioFAM/MOFA PAGA Wolf et al.16 https://github.com/theislab/paga Article ll OPEN ACCESS

    Recombinant:

    Article Title: Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CX3CR1 (8E10.D9), Purified Biolegend Cat# 824001; RRID: AB_2564876 Anti-human HLA-DR (L243), Purified Biolegend Cat# 307602; RRID: AB_314680 Anti-human IgD (IA6-2), Purified Biolegend Cat# 348202; RRID: AB_10550095 Anti-human Siglec-8 (837535), Purified R&D Systems Cat# MAB7975; RRID: N/A Anti-human TCRgd (5A6.E9), Purified Fischer Scientific Cat# TCR1061; RRID: AB_223500 Biological Samples Patients with COVID-19 from Helsinki Hospital District of Helsinki and Uusimaa, Finland N/A Chemicals, Peptides, and Recombinant Proteins Bovine Serum Albumin Sigma-Aldrich Cat# A3059; RRID: N/A Cell-ID Cisplatin Pt194 Fluidigm Cat# 201194; RRID: N/A Cell-ID Cisplatin Pt198 Fluidigm Cat# 201198; RRID: N/A Cisplatin Pt195 BuyIsotope Customized Cisplatin Pt196 BuyIsotope Customized Cell-ID Intercalator-Ir Fluidigm Cat# 201192B; RRID: N/A Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat# 201060; RRID: N/A DMSO Sigma-Aldrich Cat# D8418; RRID: N/A EDTA Rockland Cat# MB-014; RRID: N/A EQ Four Element Calibration Beads Fluidigm Cat# 201078; RRID: N/A FBS Sigma-Aldrich Cat# 12103C; RRID: N/A Fc Receptor (FcR) blocking buffer Cytodelics Customized Maxpar Cell Acquisition Solution (CAS) Fluidigm Cat# 201240; RRID; N/A Maxpar MCP9 Antibody Labeling Kit 110Cd Fluidigm Cat# 201110A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 111Cd Fluidigm Cat# 201111A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 112Cd Fluidigm Cat# 201112A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 113Cd Fluidigm Cat# 201113A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 114Cd Fluidigm Cat# 201114A; RRID: N/A Maxpar Water Fluidigm Cat# 201069; RRID; N/A Maxpar X8 Multimetal Labeling Kit (40 rxn) Fluidigm Cat# 201300; RRID; N/A Metal isotopes as chloride salts (In-115, Gd-155, Gd-157, Dy-161, Dy-163, Yb-173) Trace Sciences International Customized Paraformaldehyde VWR Cat# 16005; RRID: N/A Penicillin-streptomycin Sigma-Aldrich Cat# P4333; RRID: N/A Protein Stabilizer PBS Candor Bioscience Cat# 131125, RRID: N/A PBS 1X Rockland Cat# MB-008; RRID: N/A RPMI 1640 medium Sigma-Aldrich Cat# R848; RRID: N/A Sodium Azide Sigma-Aldrich Cat# 71289; RRID: N/A Whole blood (human) processing kit Cytodelics Cat# hC001-500; RRID: N/A Critical Commercial Assays Immune Response panel Olink AB N/A Inflammation panel Olink AB N/A Other BenchBot robot Agilent technologies Customized Bravo liquid handling platform Agilent technologies Customized (Continued on next page) e2 Cell Reports Medicine 1, 100078, August 25, 2020 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CyTOF 2 upgraded Helios mass cytometer Fluidigm N/A EL406 Washer Dispenser BioTek Customized Helios mass cytometer Fluidigm N/A pluriStrainer Mini, 40 mm pluriSelect Cat# 43-10040-70; RRID: N/A Polypropylene tubes Sarstedt Cat# 55526; RRID: N/A TC20 automated cell counter BioRad N/A Vspin microplate centrifuge Agilent technologies Customized Deposited Data FCS files, Mass cytometry This paper https://brodinlab.com/data-repository/ Protein expression data This paper https://brodinlab.com/data-repository/ IgG data This paper Table S1 Software and Algorithms CyTOF software (v. 6.5.358) N/A https://www.fluidigm.com/ Mass Cytometry Normalizer Finck et al., 201347 https://github.com/nolanlab/ bead-normalization/releases R 3.6.0 R Core Team, 2019 https://www.r-project.org/ Python 3.7.0 https://www.python.org/ Mass Cytometry Debarcoder Zunder et al., 201548 https://github.com/nolanlab/ single-cell-debarcoder CellGrid v0.5.5 Chen et al.13 https://github.com/Brodinlab/cellgrid MOFA Argelaguet et al.27 https://github.com/bioFAM/MOFA PAGA Wolf et al.16 https://github.com/theislab/paga Article ll OPEN ACCESS

    Blocking Assay:

    Article Title: Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CX3CR1 (8E10.D9), Purified Biolegend Cat# 824001; RRID: AB_2564876 Anti-human HLA-DR (L243), Purified Biolegend Cat# 307602; RRID: AB_314680 Anti-human IgD (IA6-2), Purified Biolegend Cat# 348202; RRID: AB_10550095 Anti-human Siglec-8 (837535), Purified R&D Systems Cat# MAB7975; RRID: N/A Anti-human TCRgd (5A6.E9), Purified Fischer Scientific Cat# TCR1061; RRID: AB_223500 Biological Samples Patients with COVID-19 from Helsinki Hospital District of Helsinki and Uusimaa, Finland N/A Chemicals, Peptides, and Recombinant Proteins Bovine Serum Albumin Sigma-Aldrich Cat# A3059; RRID: N/A Cell-ID Cisplatin Pt194 Fluidigm Cat# 201194; RRID: N/A Cell-ID Cisplatin Pt198 Fluidigm Cat# 201198; RRID: N/A Cisplatin Pt195 BuyIsotope Customized Cisplatin Pt196 BuyIsotope Customized Cell-ID Intercalator-Ir Fluidigm Cat# 201192B; RRID: N/A Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat# 201060; RRID: N/A DMSO Sigma-Aldrich Cat# D8418; RRID: N/A EDTA Rockland Cat# MB-014; RRID: N/A EQ Four Element Calibration Beads Fluidigm Cat# 201078; RRID: N/A FBS Sigma-Aldrich Cat# 12103C; RRID: N/A Fc Receptor (FcR) blocking buffer Cytodelics Customized Maxpar Cell Acquisition Solution (CAS) Fluidigm Cat# 201240; RRID; N/A Maxpar MCP9 Antibody Labeling Kit 110Cd Fluidigm Cat# 201110A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 111Cd Fluidigm Cat# 201111A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 112Cd Fluidigm Cat# 201112A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 113Cd Fluidigm Cat# 201113A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 114Cd Fluidigm Cat# 201114A; RRID: N/A Maxpar Water Fluidigm Cat# 201069; RRID; N/A Maxpar X8 Multimetal Labeling Kit (40 rxn) Fluidigm Cat# 201300; RRID; N/A Metal isotopes as chloride salts (In-115, Gd-155, Gd-157, Dy-161, Dy-163, Yb-173) Trace Sciences International Customized Paraformaldehyde VWR Cat# 16005; RRID: N/A Penicillin-streptomycin Sigma-Aldrich Cat# P4333; RRID: N/A Protein Stabilizer PBS Candor Bioscience Cat# 131125, RRID: N/A PBS 1X Rockland Cat# MB-008; RRID: N/A RPMI 1640 medium Sigma-Aldrich Cat# R848; RRID: N/A Sodium Azide Sigma-Aldrich Cat# 71289; RRID: N/A Whole blood (human) processing kit Cytodelics Cat# hC001-500; RRID: N/A Critical Commercial Assays Immune Response panel Olink AB N/A Inflammation panel Olink AB N/A Other BenchBot robot Agilent technologies Customized Bravo liquid handling platform Agilent technologies Customized (Continued on next page) e2 Cell Reports Medicine 1, 100078, August 25, 2020 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CyTOF 2 upgraded Helios mass cytometer Fluidigm N/A EL406 Washer Dispenser BioTek Customized Helios mass cytometer Fluidigm N/A pluriStrainer Mini, 40 mm pluriSelect Cat# 43-10040-70; RRID: N/A Polypropylene tubes Sarstedt Cat# 55526; RRID: N/A TC20 automated cell counter BioRad N/A Vspin microplate centrifuge Agilent technologies Customized Deposited Data FCS files, Mass cytometry This paper https://brodinlab.com/data-repository/ Protein expression data This paper https://brodinlab.com/data-repository/ IgG data This paper Table S1 Software and Algorithms CyTOF software (v. 6.5.358) N/A https://www.fluidigm.com/ Mass Cytometry Normalizer Finck et al., 201347 https://github.com/nolanlab/ bead-normalization/releases R 3.6.0 R Core Team, 2019 https://www.r-project.org/ Python 3.7.0 https://www.python.org/ Mass Cytometry Debarcoder Zunder et al., 201548 https://github.com/nolanlab/ single-cell-debarcoder CellGrid v0.5.5 Chen et al.13 https://github.com/Brodinlab/cellgrid MOFA Argelaguet et al.27 https://github.com/bioFAM/MOFA PAGA Wolf et al.16 https://github.com/theislab/paga Article ll OPEN ACCESS

    Antibody Labeling:

    Article Title: Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CX3CR1 (8E10.D9), Purified Biolegend Cat# 824001; RRID: AB_2564876 Anti-human HLA-DR (L243), Purified Biolegend Cat# 307602; RRID: AB_314680 Anti-human IgD (IA6-2), Purified Biolegend Cat# 348202; RRID: AB_10550095 Anti-human Siglec-8 (837535), Purified R&D Systems Cat# MAB7975; RRID: N/A Anti-human TCRgd (5A6.E9), Purified Fischer Scientific Cat# TCR1061; RRID: AB_223500 Biological Samples Patients with COVID-19 from Helsinki Hospital District of Helsinki and Uusimaa, Finland N/A Chemicals, Peptides, and Recombinant Proteins Bovine Serum Albumin Sigma-Aldrich Cat# A3059; RRID: N/A Cell-ID Cisplatin Pt194 Fluidigm Cat# 201194; RRID: N/A Cell-ID Cisplatin Pt198 Fluidigm Cat# 201198; RRID: N/A Cisplatin Pt195 BuyIsotope Customized Cisplatin Pt196 BuyIsotope Customized Cell-ID Intercalator-Ir Fluidigm Cat# 201192B; RRID: N/A Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat# 201060; RRID: N/A DMSO Sigma-Aldrich Cat# D8418; RRID: N/A EDTA Rockland Cat# MB-014; RRID: N/A EQ Four Element Calibration Beads Fluidigm Cat# 201078; RRID: N/A FBS Sigma-Aldrich Cat# 12103C; RRID: N/A Fc Receptor (FcR) blocking buffer Cytodelics Customized Maxpar Cell Acquisition Solution (CAS) Fluidigm Cat# 201240; RRID; N/A Maxpar MCP9 Antibody Labeling Kit 110Cd Fluidigm Cat# 201110A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 111Cd Fluidigm Cat# 201111A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 112Cd Fluidigm Cat# 201112A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 113Cd Fluidigm Cat# 201113A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 114Cd Fluidigm Cat# 201114A; RRID: N/A Maxpar Water Fluidigm Cat# 201069; RRID; N/A Maxpar X8 Multimetal Labeling Kit (40 rxn) Fluidigm Cat# 201300; RRID; N/A Metal isotopes as chloride salts (In-115, Gd-155, Gd-157, Dy-161, Dy-163, Yb-173) Trace Sciences International Customized Paraformaldehyde VWR Cat# 16005; RRID: N/A Penicillin-streptomycin Sigma-Aldrich Cat# P4333; RRID: N/A Protein Stabilizer PBS Candor Bioscience Cat# 131125, RRID: N/A PBS 1X Rockland Cat# MB-008; RRID: N/A RPMI 1640 medium Sigma-Aldrich Cat# R848; RRID: N/A Sodium Azide Sigma-Aldrich Cat# 71289; RRID: N/A Whole blood (human) processing kit Cytodelics Cat# hC001-500; RRID: N/A Critical Commercial Assays Immune Response panel Olink AB N/A Inflammation panel Olink AB N/A Other BenchBot robot Agilent technologies Customized Bravo liquid handling platform Agilent technologies Customized (Continued on next page) e2 Cell Reports Medicine 1, 100078, August 25, 2020 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CyTOF 2 upgraded Helios mass cytometer Fluidigm N/A EL406 Washer Dispenser BioTek Customized Helios mass cytometer Fluidigm N/A pluriStrainer Mini, 40 mm pluriSelect Cat# 43-10040-70; RRID: N/A Polypropylene tubes Sarstedt Cat# 55526; RRID: N/A TC20 automated cell counter BioRad N/A Vspin microplate centrifuge Agilent technologies Customized Deposited Data FCS files, Mass cytometry This paper https://brodinlab.com/data-repository/ Protein expression data This paper https://brodinlab.com/data-repository/ IgG data This paper Table S1 Software and Algorithms CyTOF software (v. 6.5.358) N/A https://www.fluidigm.com/ Mass Cytometry Normalizer Finck et al., 201347 https://github.com/nolanlab/ bead-normalization/releases R 3.6.0 R Core Team, 2019 https://www.r-project.org/ Python 3.7.0 https://www.python.org/ Mass Cytometry Debarcoder Zunder et al., 201548 https://github.com/nolanlab/ single-cell-debarcoder CellGrid v0.5.5 Chen et al.13 https://github.com/Brodinlab/cellgrid MOFA Argelaguet et al.27 https://github.com/bioFAM/MOFA PAGA Wolf et al.16 https://github.com/theislab/paga Article ll OPEN ACCESS

    Article Title: CyTOF protocol for immune monitoring of solid tumors from mouse models
    Article Snippet: Cell-IDTM 20-Plex Pd Barcoding Kit , Standard BioTools , 201060. .. Maxpar MCP9 Antibody Labeling Kit, 112Cd—4 Rxn , Standard BioTools , 201112A. .. Maxpar MCP9 Antibody Labeling Kit, 113Cd—4 Rxn , Standard BioTools , 201113A.

    Labeling:

    Article Title: Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-human CX3CR1 (8E10.D9), Purified Biolegend Cat# 824001; RRID: AB_2564876 Anti-human HLA-DR (L243), Purified Biolegend Cat# 307602; RRID: AB_314680 Anti-human IgD (IA6-2), Purified Biolegend Cat# 348202; RRID: AB_10550095 Anti-human Siglec-8 (837535), Purified R&D Systems Cat# MAB7975; RRID: N/A Anti-human TCRgd (5A6.E9), Purified Fischer Scientific Cat# TCR1061; RRID: AB_223500 Biological Samples Patients with COVID-19 from Helsinki Hospital District of Helsinki and Uusimaa, Finland N/A Chemicals, Peptides, and Recombinant Proteins Bovine Serum Albumin Sigma-Aldrich Cat# A3059; RRID: N/A Cell-ID Cisplatin Pt194 Fluidigm Cat# 201194; RRID: N/A Cell-ID Cisplatin Pt198 Fluidigm Cat# 201198; RRID: N/A Cisplatin Pt195 BuyIsotope Customized Cisplatin Pt196 BuyIsotope Customized Cell-ID Intercalator-Ir Fluidigm Cat# 201192B; RRID: N/A Cell-ID 20-Plex Pd Barcoding Kit Fluidigm Cat# 201060; RRID: N/A DMSO Sigma-Aldrich Cat# D8418; RRID: N/A EDTA Rockland Cat# MB-014; RRID: N/A EQ Four Element Calibration Beads Fluidigm Cat# 201078; RRID: N/A FBS Sigma-Aldrich Cat# 12103C; RRID: N/A Fc Receptor (FcR) blocking buffer Cytodelics Customized Maxpar Cell Acquisition Solution (CAS) Fluidigm Cat# 201240; RRID; N/A Maxpar MCP9 Antibody Labeling Kit 110Cd Fluidigm Cat# 201110A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 111Cd Fluidigm Cat# 201111A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 112Cd Fluidigm Cat# 201112A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 113Cd Fluidigm Cat# 201113A; RRID: N/A Maxpar MCP9 Antibody Labeling Kit 114Cd Fluidigm Cat# 201114A; RRID: N/A Maxpar Water Fluidigm Cat# 201069; RRID; N/A Maxpar X8 Multimetal Labeling Kit (40 rxn) Fluidigm Cat# 201300; RRID; N/A Metal isotopes as chloride salts (In-115, Gd-155, Gd-157, Dy-161, Dy-163, Yb-173) Trace Sciences International Customized Paraformaldehyde VWR Cat# 16005; RRID: N/A Penicillin-streptomycin Sigma-Aldrich Cat# P4333; RRID: N/A Protein Stabilizer PBS Candor Bioscience Cat# 131125, RRID: N/A PBS 1X Rockland Cat# MB-008; RRID: N/A RPMI 1640 medium Sigma-Aldrich Cat# R848; RRID: N/A Sodium Azide Sigma-Aldrich Cat# 71289; RRID: N/A Whole blood (human) processing kit Cytodelics Cat# hC001-500; RRID: N/A Critical Commercial Assays Immune Response panel Olink AB N/A Inflammation panel Olink AB N/A Other BenchBot robot Agilent technologies Customized Bravo liquid handling platform Agilent technologies Customized (Continued on next page) e2 Cell Reports Medicine 1, 100078, August 25, 2020 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER CyTOF 2 upgraded Helios mass cytometer Fluidigm N/A EL406 Washer Dispenser BioTek Customized Helios mass cytometer Fluidigm N/A pluriStrainer Mini, 40 mm pluriSelect Cat# 43-10040-70; RRID: N/A Polypropylene tubes Sarstedt Cat# 55526; RRID: N/A TC20 automated cell counter BioRad N/A Vspin microplate centrifuge Agilent technologies Customized Deposited Data FCS files, Mass cytometry This paper https://brodinlab.com/data-repository/ Protein expression data This paper https://brodinlab.com/data-repository/ IgG data This paper Table S1 Software and Algorithms CyTOF software (v. 6.5.358) N/A https://www.fluidigm.com/ Mass Cytometry Normalizer Finck et al., 201347 https://github.com/nolanlab/ bead-normalization/releases R 3.6.0 R Core Team, 2019 https://www.r-project.org/ Python 3.7.0 https://www.python.org/ Mass Cytometry Debarcoder Zunder et al., 201548 https://github.com/nolanlab/ single-cell-debarcoder CellGrid v0.5.5 Chen et al.13 https://github.com/Brodinlab/cellgrid MOFA Argelaguet et al.27 https://github.com/bioFAM/MOFA PAGA Wolf et al.16 https://github.com/theislab/paga Article ll OPEN ACCESS



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    Optimizing antibody staining and resolution in BAL cells (A) Antibody staining was tested on fresh PBMCs followed by MaxPar Fix PERM incubation with Ir 191/193 (no fix) after fixation with 2% and 4% paraformaldehyde (PFA) or BD FACS lyse buffer followed by MaxPar Fix PERM incubation with Ir 191/193 for antibodies CD4 clone RPA-T4 (row 1), CD56 clone NCAM16.2 (row 2), CD16 clone 3G8 and CD11b clone ICRF44 (row 3). Staining after fixative treatment reduced the resolution of these antibody clones compared to fresh cells. (B) Titrations of surface marker CD206 and intra-cellular marker IL1ß on BAL cells highlights the importance of titrating markers on both stimulated and unstimulated cells. (C) BAL or PBMCs were stained with the full antibody cocktail (stained) and compared to unstained controls (Ir 191/193 staining only) to determine true positive signal for markers CD19 and CD32 (row 1), CD19 and CD40 (row 2), CD11c and CD89 (row 3).

    Journal: STAR Protocols

    Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

    doi: 10.1016/j.xpro.2024.103463

    Figure Lengend Snippet: Optimizing antibody staining and resolution in BAL cells (A) Antibody staining was tested on fresh PBMCs followed by MaxPar Fix PERM incubation with Ir 191/193 (no fix) after fixation with 2% and 4% paraformaldehyde (PFA) or BD FACS lyse buffer followed by MaxPar Fix PERM incubation with Ir 191/193 for antibodies CD4 clone RPA-T4 (row 1), CD56 clone NCAM16.2 (row 2), CD16 clone 3G8 and CD11b clone ICRF44 (row 3). Staining after fixative treatment reduced the resolution of these antibody clones compared to fresh cells. (B) Titrations of surface marker CD206 and intra-cellular marker IL1ß on BAL cells highlights the importance of titrating markers on both stimulated and unstimulated cells. (C) BAL or PBMCs were stained with the full antibody cocktail (stained) and compared to unstained controls (Ir 191/193 staining only) to determine true positive signal for markers CD19 and CD32 (row 1), CD19 and CD40 (row 2), CD11c and CD89 (row 3).

    Article Snippet: Maxpar MCP9 antibody labeling kit, 112Cd , Standard BioTools , Cat# 201112A.

    Techniques: Staining, Incubation, Clone Assay, Marker

    Journal: STAR Protocols

    Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

    doi: 10.1016/j.xpro.2024.103463

    Figure Lengend Snippet:

    Article Snippet: Maxpar MCP9 antibody labeling kit, 112Cd , Standard BioTools , Cat# 201112A.

    Techniques: Recombinant, Staining, Saline, Software, Antibody Labeling, Spectrophotometry, Hood, Microscopy, Membrane, Sterility, Transferring

    Mass cytometry chemokine receptor cocktail

    Journal: STAR Protocols

    Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

    doi: 10.1016/j.xpro.2024.103463

    Figure Lengend Snippet: Mass cytometry chemokine receptor cocktail

    Article Snippet: Maxpar MCP9 antibody labeling kit, 112Cd , Standard BioTools , Cat# 201112A.

    Techniques: Mass Cytometry, Concentration Assay, Staining

    Mass cytometry surface antibody cocktail

    Journal: STAR Protocols

    Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

    doi: 10.1016/j.xpro.2024.103463

    Figure Lengend Snippet: Mass cytometry surface antibody cocktail

    Article Snippet: Maxpar MCP9 antibody labeling kit, 112Cd , Standard BioTools , Cat# 201112A.

    Techniques: Mass Cytometry, Concentration Assay, Staining

    Mass cytometry intra-cellular antibody cocktail

    Journal: STAR Protocols

    Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

    doi: 10.1016/j.xpro.2024.103463

    Figure Lengend Snippet: Mass cytometry intra-cellular antibody cocktail

    Article Snippet: Maxpar MCP9 antibody labeling kit, 112Cd , Standard BioTools , Cat# 201112A.

    Techniques: Mass Cytometry, Concentration Assay

    Journal: STAR Protocols

    Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

    doi: 10.1016/j.xpro.2024.103463

    Figure Lengend Snippet:

    Article Snippet: Maxpar MCP9 antibody labeling kit, 112Cd , Standard BioTools , Cat# 201112A.

    Techniques: Recombinant, Staining, Saline, Software, Antibody Labeling, Spectrophotometry, Hood, Microscopy, Membrane, Sterility, Transferring

    Journal: STAR Protocols

    Article Title: CyTOF protocol for immune monitoring of solid tumors from mouse models

    doi: 10.1016/j.xpro.2022.101949

    Figure Lengend Snippet:

    Article Snippet: Maxpar MCP9 Antibody Labeling Kit, 112Cd—4 Rxn , Standard BioTools , 201112A.

    Techniques: Blocking Assay, Purification, Recombinant, Staining, Sterility, Antibody Labeling, Software, Transferring, Microscopy