phage ef113 atcc 19950 b1  (ATCC)

Journal: Antibiotics

Article Title: Bacteriophage Therapy as a Promising Alternative for Antibiotic-Resistant Enterococcus faecium : Advances and Challenges

doi: 10.3390/antibiotics13121120

Figure Lengend Snippet: Summary of in vitro studies evaluating phage therapy against E. faecium .

Article Snippet: 23 , ATCC 19950-B1 (113) , ATCC 19950, Com12, 1.141.733 , TKA , Phage alone did not eradicate bacteria. DAP + phage reduced 3.0–3.5 log10 CFU/mL. , Synergy with DAP and prevention of phage 9184 resistance. , Phage 113 and DAP showed bactericidal activity and prevented resistance. , [ ] .

Techniques: In Vitro, Bacteria, Purification, Modification, Activity Assay, Sensitive Assay, Plaque Assay, Inhibition, Titration, Adsorption, Isolation, Mutagenesis, Lysis, Disruption, Sequencing, Viability Assay, Drug Susceptibility Assay

Journal: Nature reviews. Microbiology

Article Title: Enterococcus faecium : evolution, adaptation, pathogenesis and emerging therapeutics

doi: 10.1038/s41579-024-01058-6

Figure Lengend Snippet:

Article Snippet: Phage-antibiotic synergy , E. faecium bacteriophage 113 (ATCC 19950-B1) with daptomycin and/or β-lactams are effective in killing both daptomycin-sensitive and daptomycin-resistant E. faecium . 194 , Genomic mutations provide resistance to phage sensitize the bacteria to the antibiotics, and vice versa..

Techniques: DNA-DNA Hybridization, Sequencing, Comparison, Isolation

Journal: Antibiotics

Article Title: Phage Cocktails with Daptomycin and Ampicillin Eradicates Biofilm-Embedded Multidrug-Resistant Enterococcus faecium with Preserved Phage Susceptibility

doi: 10.3390/antibiotics11091175

Figure Lengend Snippet: Biofilm quantification (optical density (OD), compared to control) and phage susceptibility against E. faecium clinical isolates.

Article Snippet: ATCC 113 Phage (ATCC 19950-B1) and propagating organism E. faecium (ATCC 19950) were purchased from ATCC (Manassas, VA, USA).

Techniques:

Journal: Antibiotics

Article Title: Phage Cocktails with Daptomycin and Ampicillin Eradicates Biofilm-Embedded Multidrug-Resistant Enterococcus faecium with Preserved Phage Susceptibility

doi: 10.3390/antibiotics11091175

Figure Lengend Snippet: ( A , B ). Modified checkerboard minimum biofilm inhibitory concentration (MBIC) analyses of daptomycin combined with phage NV-497 ( A ) and a 4-phage cocktail of ATCC 113 + NV-497 + NV-503-01 + NV-503-02 ( B ) (each phage at MOI 0.01) against DAP-resistant R497. Additivity, defined as an FIC index >0.5 but <4, is indicated by the red outline in ( A ). Synergy, defined as an FIC index ≤0.5, is indicated by the red outline in ( B ). Comparisons are versus growth control and depicted by the purple color gradient as percent of growth.

Article Snippet: ATCC 113 Phage (ATCC 19950-B1) and propagating organism E. faecium (ATCC 19950) were purchased from ATCC (Manassas, VA, USA).

Techniques: Modification, Concentration Assay

Journal: Antibiotics

Article Title: Phage Cocktails with Daptomycin and Ampicillin Eradicates Biofilm-Embedded Multidrug-Resistant Enterococcus faecium with Preserved Phage Susceptibility

doi: 10.3390/antibiotics11091175

Figure Lengend Snippet: ( A , B ). Modified checkerboard minimum biofilm inhibitory concentration (MBIC) analyses of combination daptomycin and ampicillin without ( A ) and a phage cocktail of ATCC 113 + NV-497 + NV-503-01 ( B ) (each at MOI 0.01) against DAP-resistant R497. Synergy, defined as an FIC index ≤0.5, is indicated by the red outline in ( A , B ). Comparisons are versus growth control and depicted by the purple color gradient as percent of growth.

Article Snippet: ATCC 113 Phage (ATCC 19950-B1) and propagating organism E. faecium (ATCC 19950) were purchased from ATCC (Manassas, VA, USA).

Techniques: Modification, Concentration Assay

Journal: Antibiotics

Article Title: Phage Cocktails with Daptomycin and Ampicillin Eradicates Biofilm-Embedded Multidrug-Resistant Enterococcus faecium with Preserved Phage Susceptibility

doi: 10.3390/antibiotics11091175

Figure Lengend Snippet: Time kill analyses of DAP at 0.5x MBIC, AMP at free peak = 72 µg/mL, and DAP + AMP, alone and in combination with four bacteriophages, ATCC 113, NV-497, NV-503-01, NV-503-02, each at an MOI of 0.01 against DAP-resistant R497. Change in CFU/mL was in comparison to initial inoculum. Synergy was defined as a ≥2-log 10 -CFU/mL kill compared to the most effective agent (or double-combination regimen) at 24 h (synergistic regimens indicated by black circle). Bactericidal activity was defined as a ≥3-log 10 -CFU/mL reduction from baseline (bactericidal regimens indicated by the bars at or extending below the dashed black line).

Article Snippet: ATCC 113 Phage (ATCC 19950-B1) and propagating organism E. faecium (ATCC 19950) were purchased from ATCC (Manassas, VA, USA).

Techniques: Activity Assay

Journal: Antibiotics

Article Title: Phage Cocktails with Daptomycin and Ampicillin Eradicates Biofilm-Embedded Multidrug-Resistant Enterococcus faecium with Preserved Phage Susceptibility

doi: 10.3390/antibiotics11091175

Figure Lengend Snippet: Time kill analyses of DAP at 0.5x MBIC, AMP at free peak = 72 µg/mL, and DAP + AMP, alone and in combination with four bacteriophages, ATCC 113, NV-497, NV-503-01, NV-503-02, each at an MOI of 0.01 against DAP-susceptible dose-dependent HOU503. Change in CFU/mL was in comparison to initial inoculum. Synergy was defined as a ≥2-log 10 -CFU/mL kill compared to the most effective agent (or double-combination regimen) at 24 h (synergistic regimens indicated by black circle). Bactericidal activity was defined as a ≥3-log 10 -CFU/mL reduction from baseline (bactericidal regimens indicated by the bars at or extending below the dashed black line).

Article Snippet: ATCC 113 Phage (ATCC 19950-B1) and propagating organism E. faecium (ATCC 19950) were purchased from ATCC (Manassas, VA, USA).

Techniques: Activity Assay

Journal: Antibiotics

Article Title: Phage Cocktails with Daptomycin and Ampicillin Eradicates Biofilm-Embedded Multidrug-Resistant Enterococcus faecium with Preserved Phage Susceptibility

doi: 10.3390/antibiotics11091175

Figure Lengend Snippet: Evaluation of phage resistance in R497 and HOU503 at the end of 24 h time kill analyses. Phages are represented in the table as follows: 1, ATCC 113; 2, NV-497; 3, NV-503-01; 4, NV-503-02.

Article Snippet: ATCC 113 Phage (ATCC 19950-B1) and propagating organism E. faecium (ATCC 19950) were purchased from ATCC (Manassas, VA, USA).

Techniques: