Journal: mBio
Article Title: Terminal complement complexes with or without C9 potentiate antimicrobial activity against Neisseria gonorrhoeae
doi: 10.1128/mbio.00141-25
Figure Lengend Snippet: MAC-dependent increase in sensitivity and susceptibility of multidrug-resistant Gc to frontline antibiotics. (A–C) H041 Gc was pre-incubated with anti-Gc IgM followed by incubation with 2% (A, C) or indicated concentration (B) of IgG/M-depleted human serum, with or without heat inactivation (HI). Gc was then incubated with the indicated antibiotic, and CFUs were enumerated. Where indicated, serum was first incubated with the C5 inhibitor OMCI (20 µg/mL) or vehicle. Error bars are standard error of the mean. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons on log 10 -transformed data. ** P < 0.01 and **** P < 0.0001. Dotted line represents minimum reportable CFUs. (D, E) FA19 Gc (D) or H041 Gc (E) were assayed via 16 h minimum inhibitory concentration (MIC) broth microdilution assays over a range of azithromycin or ceftriaxone concentrations in GCBL alone, or supplemented with 2.5% IgG/M-depleted human serum.
Article Snippet: Ceftriaxone , Cayman , 18866 , , , , .
Techniques: Incubation, Concentration Assay, Transformation Assay