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compound 11a  (ATCC)


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    ATCC compound 11a
    Compound 11a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 38893 article reviews
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    99/100 stars

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    Image Search Results


    a, Workflow for endogenous knockout validation using SARS-CoV-2 virus infection. b, RNA-seq heat-map of gene expression in representative cell lines alongside cDNA-overexpressing reference lines. c-d , SARS-CoV-2 live virus infection of NCC-Stc-K140 (c) and SW156 (d) cells perturbed with CRISPR-based loss-of-function constructs. Three guideRNAs were used per gene and results for the three independent lines were performed via individual arrayed infections and pooled for analysis. The entire infection was repeated twice to collect replicates for all cell lines. Results are normed to the BFP control/vehicle for each respective pseudotype. e, Data showing the dosage-dependent effect on infection of SARS-CoV-2 Spike-D614G or VSV-G pseudotyped lentivirus in LGMN-expressing SW156 cells treated with RR-11a, a specific inhibitor of legumain (LGMN). f, Structure and domains of human Legumain. SP: signal peptide, NTF: N-terminal fragment, CD: catalytic domain, AP: activation peptide, LSAM: legumain stabilization and activity modulation domain. g-h, SARS-CoV-2 live virus infection of ACE2OE 293FT cells expressing LGMN or LGMN catalytic (g) and signal peptide (h) mutant cDNA. Results are normed to BFP control. Data in (g) from three independent experiments and (h) from two independent experiments. All data represent mean with SEM. Statistical analyses: for c-d,f performed via one-way ANOVA with BFP or non-target as the control condition and for e, g-h with two-way ANOVA, all with correction for multiple comparisons during hypothesis testing. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

    Journal: PLOS Pathogens

    Article Title: Membrane-wide screening identifies potential tissue-specific determinants of SARS-CoV-2 tropism

    doi: 10.1371/journal.ppat.1013157

    Figure Lengend Snippet: a, Workflow for endogenous knockout validation using SARS-CoV-2 virus infection. b, RNA-seq heat-map of gene expression in representative cell lines alongside cDNA-overexpressing reference lines. c-d , SARS-CoV-2 live virus infection of NCC-Stc-K140 (c) and SW156 (d) cells perturbed with CRISPR-based loss-of-function constructs. Three guideRNAs were used per gene and results for the three independent lines were performed via individual arrayed infections and pooled for analysis. The entire infection was repeated twice to collect replicates for all cell lines. Results are normed to the BFP control/vehicle for each respective pseudotype. e, Data showing the dosage-dependent effect on infection of SARS-CoV-2 Spike-D614G or VSV-G pseudotyped lentivirus in LGMN-expressing SW156 cells treated with RR-11a, a specific inhibitor of legumain (LGMN). f, Structure and domains of human Legumain. SP: signal peptide, NTF: N-terminal fragment, CD: catalytic domain, AP: activation peptide, LSAM: legumain stabilization and activity modulation domain. g-h, SARS-CoV-2 live virus infection of ACE2OE 293FT cells expressing LGMN or LGMN catalytic (g) and signal peptide (h) mutant cDNA. Results are normed to BFP control. Data in (g) from three independent experiments and (h) from two independent experiments. All data represent mean with SEM. Statistical analyses: for c-d,f performed via one-way ANOVA with BFP or non-target as the control condition and for e, g-h with two-way ANOVA, all with correction for multiple comparisons during hypothesis testing. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

    Article Snippet: RR-11a (MedChemExpress, HY-112205A) was diluted in DMSO from a stock concentration of 100mM.

    Techniques: Knock-Out, Biomarker Discovery, Virus, Infection, RNA Sequencing, Gene Expression, CRISPR, Construct, Control, Expressing, Activation Assay, Activity Assay, Mutagenesis