t3 atcc 11303 b3  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Local Bacteriophage Delivery for Treatment and Prevention of Bacterial Infections

doi: 10.3389/fmicb.2020.538060

Figure Lengend Snippet: Overview of biopolymers used for bacteriophage embedding.

Article Snippet: Phage delivery for internal medicine , Liposomes containing: ∙ DSCP phospholipids ∙ Cholesterol , S. aureus phage K ATCC 1985-B1 and E. coli phage ATCC 11303-B3 , In vitro , Liposomes were fabricated using microfluidic hydrodynamic flow focusing, with a phage load of ∼10 8 PFU/mL , Encapsulation in liposomes offered limited protection against acidic environments, with a 3log 10 reduction of phage. , Not assessed , .

Techniques: Viscosity, In Vitro, Incubation, Control, Preserving, In Vivo, Ex Vivo, Bacteria, CRISPR, Modification, Cream

Journal: Frontiers in Microbiology

Article Title: Local Bacteriophage Delivery for Treatment and Prevention of Bacterial Infections

doi: 10.3389/fmicb.2020.538060

Figure Lengend Snippet: Overview of the use of synthetic polymers for bacteriophage embedding.

Article Snippet: Phage delivery for internal medicine , Liposomes containing: ∙ DSCP phospholipids ∙ Cholesterol , S. aureus phage K ATCC 1985-B1 and E. coli phage ATCC 11303-B3 , In vitro , Liposomes were fabricated using microfluidic hydrodynamic flow focusing, with a phage load of ∼10 8 PFU/mL , Encapsulation in liposomes offered limited protection against acidic environments, with a 3log 10 reduction of phage. , Not assessed , .

Techniques: In Vitro, Encapsulation, Preserving, Isolation, Activity Assay, In Vivo, Dispersion, Incubation, Infection, Formulation, Control, Adhesive

Journal: Frontiers in Microbiology

Article Title: Local Bacteriophage Delivery for Treatment and Prevention of Bacterial Infections

doi: 10.3389/fmicb.2020.538060

Figure Lengend Snippet: Overview of the use of liposomes for bacteriophage encapsulation.

Article Snippet: Phage delivery for internal medicine , Liposomes containing: ∙ DSCP phospholipids ∙ Cholesterol , S. aureus phage K ATCC 1985-B1 and E. coli phage ATCC 11303-B3 , In vitro , Liposomes were fabricated using microfluidic hydrodynamic flow focusing, with a phage load of ∼10 8 PFU/mL , Encapsulation in liposomes offered limited protection against acidic environments, with a 3log 10 reduction of phage. , Not assessed , .

Techniques: Liposomes, Encapsulation, In Vitro, Homogenization, In Vivo, Control, Infection, Labeling, Marker, Bacteria, Emulsion, Confocal Microscopy

Journal: Frontiers in Microbiology

Article Title: Bacteriophage Production Models: An Overview

doi: 10.3389/fmicb.2019.01187

Figure Lengend Snippet: Production data available on bacteriophage production cases evaluated experimentally.

Article Snippet: Escherichia coli ATCC 11303 – Phage T3 ATCC 11303-B3 , Production : 10 11 PFU mL –1 Working Volume : 1 L (bioreactor) , .

Techniques:

Journal: Viruses

Article Title: High Throughput Manufacturing of Bacteriophages Using Continuous Stirred Tank Bioreactors Connected in Series to Ensure Optimum Host Bacteria Physiology for Phage Production

doi: 10.3390/v10100537

Figure Lengend Snippet: Schematic showing layout of the continuous phage production process, which was a three-stage continuous stirred tank system with bioreactors connected in series: the first reactor (Bioreactor 1, R1) had a fixed working volume of 500 mL, the second reactor (Bioreactor 2, R2) had variable working volumes ranging from 33 mL to 100 mL, thereby permitting different dilution rates (D 2 ) for phage production using the same flow rate through the process, and the third reactor (Bioreactor 3, R3) was operated as a semi-batch reactor. Host E. coli were propagated in R1; the working volume was controlled using a level control system. The T3 infection of bacteria occurred in R2, which was fed with host bacteria continuously from R1. R2 was operated below the dilution rate where phage washout would occur; hence, steady-state operation allowed the phage population to be continuously maintained in R2. Phage-infected cells and free-floating phage were continuously withdrawn from R2 to R3 (flow rate synchronized with R1 using a level control system on R2) where multiple cycles of infection resulted in further phage amplification and completion of the phage production process.

Article Snippet: Host bacterium E. coli (ATCC 11303) and its lytic phage T3 (ATCC11303-B3), belonging to the Podoviridae family, were sourced from LGC standards (Teddington, Middlesex, United Kingdom (UK)).

Techniques: Control, Infection, Bacteria, Amplification