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wizard lambda prep dna purification kit  (Promega)

 
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    Promega wizard lambda prep dna purification kit
    Wizard Lambda Prep Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wizard+lambda+preps+kit/wizard+lambda+preps+dna+purification+system/us07956237-2548-21-27
    Average 90 stars, based on 1 article reviews
    wizard lambda prep dna purification kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP1 and DLP2 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [60, 61]. .. A 10 ml aliquot of filtersterilized phage lysate (propagated on D1585 with agarose medium) was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 °C to degrade the bacterial nucleic acids.

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: .. Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. φMSHR5848 DNA isolation, sequencing and analysis Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [23, 24]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 ̊C to degrade the bacterial nucleic acids.

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.

    Sequencing:

    Article Title: The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP1 and DLP2 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [60, 61]. .. A 10 ml aliquot of filtersterilized phage lysate (propagated on D1585 with agarose medium) was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 °C to degrade the bacterial nucleic acids.

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: .. Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. φMSHR5848 DNA isolation, sequencing and analysis Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [23, 24]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 ̊C to degrade the bacterial nucleic acids.

    Isolation:

    Article Title: The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP1 and DLP2 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [60, 61]. .. A 10 ml aliquot of filtersterilized phage lysate (propagated on D1585 with agarose medium) was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 °C to degrade the bacterial nucleic acids.

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6
    Article Snippet: .. DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [ , ]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37°C to degrade the bacterial nucleic acids.

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [23, 24]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 ̊C to degrade the bacterial nucleic acids.

    Article Title: The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1.
    Article Snippet: The average capsid and tail dimensions ± standard deviation was calculated using Microsoft Excel based on measurements from 10 individual virions taken using ImageJ software (NIH, Bethesda, MD, USA). .. JC1 genomic DNA (gDNA) was isolated from a high titer phage stock using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI, USA) with some modifications. .. JC1 genomic DNA (gDNA) was isolated from a high titer phage stock using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI, USA) with some modifications.

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.

    Lambda DNA Preparation:

    Article Title: The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP1 and DLP2 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [60, 61]. .. A 10 ml aliquot of filtersterilized phage lysate (propagated on D1585 with agarose medium) was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 °C to degrade the bacterial nucleic acids.

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6
    Article Snippet: .. DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [ , ]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37°C to degrade the bacterial nucleic acids.

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [23, 24]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 ̊C to degrade the bacterial nucleic acids.

    Article Title: The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1.
    Article Snippet: The average capsid and tail dimensions ± standard deviation was calculated using Microsoft Excel based on measurements from 10 individual virions taken using ImageJ software (NIH, Bethesda, MD, USA). .. JC1 genomic DNA (gDNA) was isolated from a high titer phage stock using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI, USA) with some modifications. .. JC1 genomic DNA (gDNA) was isolated from a high titer phage stock using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI, USA) with some modifications.

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.

    Purification:

    Article Title: The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP1 and DLP2 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [60, 61]. .. A 10 ml aliquot of filtersterilized phage lysate (propagated on D1585 with agarose medium) was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 °C to degrade the bacterial nucleic acids.

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: .. Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. φMSHR5848 DNA isolation, sequencing and analysis Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6
    Article Snippet: .. DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [ , ]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37°C to degrade the bacterial nucleic acids.

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. .. Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [23, 24]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 ̊C to degrade the bacterial nucleic acids.

    Article Title: The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1.
    Article Snippet: The average capsid and tail dimensions ± standard deviation was calculated using Microsoft Excel based on measurements from 10 individual virions taken using ImageJ software (NIH, Bethesda, MD, USA). .. JC1 genomic DNA (gDNA) was isolated from a high titer phage stock using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI, USA) with some modifications. .. JC1 genomic DNA (gDNA) was isolated from a high titer phage stock using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI, USA) with some modifications.

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.

    Modification:

    Article Title: The isolation and characterization of two Stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP1 and DLP2 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [60, 61]. .. A 10 ml aliquot of filtersterilized phage lysate (propagated on D1585 with agarose medium) was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 °C to degrade the bacterial nucleic acids.

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6
    Article Snippet: .. DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [ , ]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37°C to degrade the bacterial nucleic acids.

    Article Title: The isolation and characterization of Stenotrophomonas maltophilia T4-like bacteriophage DLP6.
    Article Snippet: .. Phage DNA isolation, RFLP analysis and sequencing DLP6 genomic DNA was isolated from bacteriophage lysate using the Wizard Lambda DNA purification system (Promega Corp., Madison, WI) with a modified protocol [23, 24]. .. A 10 ml aliquot of high-titre filter-sterilized phage lysate was treated with 10 μl DNase I (Thermo Scientific, Waltham, MA), 100 μl 100x DNase I buffer (1 M Tris–HCl, 0.25 M MgCl2, 10 mM CaCl2), and 6 μl RNase (Thermo Scientific) and incubated 1 h at 37 ̊C to degrade the bacterial nucleic acids.

    Transmission Assay:

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: .. Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. φMSHR5848 DNA isolation, sequencing and analysis Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    Microscopy:

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: .. Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. φMSHR5848 DNA isolation, sequencing and analysis Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    DNA Purification:

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: .. Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. φMSHR5848 DNA isolation, sequencing and analysis Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    Article Title: Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
    Article Snippet: Samples were evaluated on a JEOL 1011 transmission electron microscope at 80 kV and digital images were acquired using an AMT camera system. .. Bacteriophage DNA was purified from a B. mallei ATCC 23344 plate culture lysate using the Wizard lambda preps DNA purification system (Promega). ..

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.

    other:

    Article Title: The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1
    Article Snippet: The average capsid and tail dimensions ± standard deviation was calculated using Microsoft Excel based on measurements from 10 individual virions taken using ImageJ software (NIH, Bethesda, MD, USA).

    Plasmid Preparation:

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.

    Polymerase Chain Reaction:

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.

    Ligation:

    Article Title: Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei : temperature regulates the expression of a two-component signal transduction system
    Article Snippet: .. Plasmid DNA was isolated using the Miniprep plasmid DNA extraction kit (BioRad), lambda DNA was purified using the Wizard λ prep DNA purification kit (Promega) and DNA fragments, PCR products and ligation mixtures were recovered using the Prep-a-gene DNA purification kit (BioRad). .. Genomic DNA was extracted using the Puregene D-6000 DNA isolation kit (Gentra Systems) and quantified by comparison with lambda DNA standards (Sigma Aldrich) using digital images of 1% agarose gels stained with ethidium bromide.



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