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Amersham Life Sciences Inc microarray hybridization buffer version 2
Microarray Hybridization Buffer Version 2, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+2+microarray+hybridization+buffer/microarray+hybridization+buffer/pm21130852-95-17-23
Average 90 stars, based on 1 article reviews
microarray hybridization buffer version 2 - by Bioz Stars, 2026-09
90/100 stars

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Labeling:

Article Title: Gene relating to estimation of postoperative prognosis for breast cancer
Article Snippet: For removing an unbound dye, a labeled probe was purified by QIA quick PCR purification kit (QIAGEN, Valencia, Calif.). .. Each 10 pmol of fluorescent labeled probes from tumor and normal RNA were mixed with 4× microarray hybridization buffer (Amersham (UK)) and de-ionized formamide. ..

Article Title: The involvement of N-G,N-G-dimethyarginine dimethylhydrolase 1 in the proliferative effect of Astragali radix on cardiac cells.
Article Snippet: Aim of the study: Astragali radix (AR) is a widely used traditional medicine in oriental countries for treating various diseases including cardiovascular disease (CVD).. We investigated the effects of AR extracts on rat cardiomyocytes and H9C2 cardiac cells as well as identified many target genes that mediate the effect of AR.. Materials and methods: The effect of AR extracts on cell proliferation was assessed and cDNA microarray technique was used to analyse the differential gene expressions upon AR treatment in cardiac cells.

Microarray:

Article Title: Gene relating to estimation of postoperative prognosis for breast cancer
Article Snippet: For removing an unbound dye, a labeled probe was purified by QIA quick PCR purification kit (QIAGEN, Valencia, Calif.). .. Each 10 pmol of fluorescent labeled probes from tumor and normal RNA were mixed with 4× microarray hybridization buffer (Amersham (UK)) and de-ionized formamide. ..

Article Title: The Transcriptional Repressor ARR1-SRDX Suppresses Pleiotropic Cytokinin Activities in Arabidopsis
Article Snippet: .. Forty picomoles of incorporated Cy5 and 1 pmol Cy3-labeled oligonucleotide reference were dissolved in a mixture of 25% Microarray Hybridization Buffer (Amersham), 50% formamide, and 33.3 ng μ L −1 poly(dT 35 ) DNA, applied onto CATMA V2.4 microarrays (ArrayExpress accession no. A-MEXP-274 ; http://www.ebi.ac.uk/aerep ), covered with a coverslip, and incubated in hybridization chambers (Scienion) in a water bath at 42°C for 15 h. The arrays were washed once with 1× SSC, 0.2% SDS for 10 min at 55°C, twice with 0.1× SSC, 0.2% SDS for 10 min at 55°C, and once with 0.1× SSC at 37°C and finally dipped five times into distilled water at room temperature. ..

Article Title: Molecular basis of Tropheryma whipplei doxycycline susceptibility examined by transcriptional profiling.
Article Snippet: .. The mixture was heated at 958C for 2 min and cooled on ice for 30 s before addition of 7.5 mL of microarray hybridization buffer (supplied in the CyScribe kit; Amersham) and 15 mL of 100% (v/v) formamide. ..

Article Title: Androgen regulated prostate specific nucleic acids
Article Snippet: .. One μg of dA/dT 12-18 (Pharmacia, Kalamazoo, Mich.) and 1 μg of Cot1 DNA (Life Technologies, Germantown, Md.) were added to the probe, heat denatured at 94° C. for minutes, combined with an equal volume of 2× microarray hybridization solution (Amersham, Piscataway, N.J.) and placed onto the microarray slide with a coverslip. ..

Article Title: The involvement of N-G,N-G-dimethyarginine dimethylhydrolase 1 in the proliferative effect of Astragali radix on cardiac cells.
Article Snippet: Aim of the study: Astragali radix (AR) is a widely used traditional medicine in oriental countries for treating various diseases including cardiovascular disease (CVD).. We investigated the effects of AR extracts on rat cardiomyocytes and H9C2 cardiac cells as well as identified many target genes that mediate the effect of AR.. Materials and methods: The effect of AR extracts on cell proliferation was assessed and cDNA microarray technique was used to analyse the differential gene expressions upon AR treatment in cardiac cells.

Article Title: Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays.
Article Snippet: Ellestad, Laura E., Wilfrid Carre, Michael Muchow, Sultan A. Jenkins, Xiaofei Wang, Larry A. Cogburn, and Tom E. Porter.. Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays.. Physiol Genomics 25: 414–425, 2006.

Article Title: Development of a method for recovering rickettsial RNA from infected cells to analyze gene expression profiling of obligate intracellular bacteria.
Article Snippet: The Rickettsia genus is composed of Gram-negative bacteria responsible for Typhus and spotted fevers.. Because of the limitations imposed by their obligate intracellular location, the molecular mechanisms responsible for their pathogenicity remain poorly understood.. Several rickettsial genomes are now available, thus providing the foundation for a new era of post-genomic research.

Article Title: SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function.
Article Snippet: Fontijn RD, Volger OL, Fledderus JO, Reijerkerk A, de Vries HE, Horrevoets AJ.. SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function.. Am J Physiol Heart Circ Physiol 294: H891–H900, 2008.

Hybridization:

Article Title: Gene relating to estimation of postoperative prognosis for breast cancer
Article Snippet: For removing an unbound dye, a labeled probe was purified by QIA quick PCR purification kit (QIAGEN, Valencia, Calif.). .. Each 10 pmol of fluorescent labeled probes from tumor and normal RNA were mixed with 4× microarray hybridization buffer (Amersham (UK)) and de-ionized formamide. ..

Article Title: The Transcriptional Repressor ARR1-SRDX Suppresses Pleiotropic Cytokinin Activities in Arabidopsis
Article Snippet: .. Forty picomoles of incorporated Cy5 and 1 pmol Cy3-labeled oligonucleotide reference were dissolved in a mixture of 25% Microarray Hybridization Buffer (Amersham), 50% formamide, and 33.3 ng μ L −1 poly(dT 35 ) DNA, applied onto CATMA V2.4 microarrays (ArrayExpress accession no. A-MEXP-274 ; http://www.ebi.ac.uk/aerep ), covered with a coverslip, and incubated in hybridization chambers (Scienion) in a water bath at 42°C for 15 h. The arrays were washed once with 1× SSC, 0.2% SDS for 10 min at 55°C, twice with 0.1× SSC, 0.2% SDS for 10 min at 55°C, and once with 0.1× SSC at 37°C and finally dipped five times into distilled water at room temperature. ..

Article Title: Molecular basis of Tropheryma whipplei doxycycline susceptibility examined by transcriptional profiling.
Article Snippet: .. The mixture was heated at 958C for 2 min and cooled on ice for 30 s before addition of 7.5 mL of microarray hybridization buffer (supplied in the CyScribe kit; Amersham) and 15 mL of 100% (v/v) formamide. ..

Article Title: Androgen regulated prostate specific nucleic acids
Article Snippet: .. One μg of dA/dT 12-18 (Pharmacia, Kalamazoo, Mich.) and 1 μg of Cot1 DNA (Life Technologies, Germantown, Md.) were added to the probe, heat denatured at 94° C. for minutes, combined with an equal volume of 2× microarray hybridization solution (Amersham, Piscataway, N.J.) and placed onto the microarray slide with a coverslip. ..

Article Title: The involvement of N-G,N-G-dimethyarginine dimethylhydrolase 1 in the proliferative effect of Astragali radix on cardiac cells.
Article Snippet: Aim of the study: Astragali radix (AR) is a widely used traditional medicine in oriental countries for treating various diseases including cardiovascular disease (CVD).. We investigated the effects of AR extracts on rat cardiomyocytes and H9C2 cardiac cells as well as identified many target genes that mediate the effect of AR.. Materials and methods: The effect of AR extracts on cell proliferation was assessed and cDNA microarray technique was used to analyse the differential gene expressions upon AR treatment in cardiac cells.

Article Title: Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays.
Article Snippet: Ellestad, Laura E., Wilfrid Carre, Michael Muchow, Sultan A. Jenkins, Xiaofei Wang, Larry A. Cogburn, and Tom E. Porter.. Gene expression profiling during cellular differentiation in the embryonic pituitary gland using cDNA microarrays.. Physiol Genomics 25: 414–425, 2006.

Article Title: Development of a method for recovering rickettsial RNA from infected cells to analyze gene expression profiling of obligate intracellular bacteria.
Article Snippet: The Rickettsia genus is composed of Gram-negative bacteria responsible for Typhus and spotted fevers.. Because of the limitations imposed by their obligate intracellular location, the molecular mechanisms responsible for their pathogenicity remain poorly understood.. Several rickettsial genomes are now available, thus providing the foundation for a new era of post-genomic research.

Article Title: SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function.
Article Snippet: Fontijn RD, Volger OL, Fledderus JO, Reijerkerk A, de Vries HE, Horrevoets AJ.. SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function.. Am J Physiol Heart Circ Physiol 294: H891–H900, 2008.

Incubation:

Article Title: The Transcriptional Repressor ARR1-SRDX Suppresses Pleiotropic Cytokinin Activities in Arabidopsis
Article Snippet: .. Forty picomoles of incorporated Cy5 and 1 pmol Cy3-labeled oligonucleotide reference were dissolved in a mixture of 25% Microarray Hybridization Buffer (Amersham), 50% formamide, and 33.3 ng μ L −1 poly(dT 35 ) DNA, applied onto CATMA V2.4 microarrays (ArrayExpress accession no. A-MEXP-274 ; http://www.ebi.ac.uk/aerep ), covered with a coverslip, and incubated in hybridization chambers (Scienion) in a water bath at 42°C for 15 h. The arrays were washed once with 1× SSC, 0.2% SDS for 10 min at 55°C, twice with 0.1× SSC, 0.2% SDS for 10 min at 55°C, and once with 0.1× SSC at 37°C and finally dipped five times into distilled water at room temperature. ..

Fluorescence:

Article Title: The involvement of N-G,N-G-dimethyarginine dimethylhydrolase 1 in the proliferative effect of Astragali radix on cardiac cells.
Article Snippet: Aim of the study: Astragali radix (AR) is a widely used traditional medicine in oriental countries for treating various diseases including cardiovascular disease (CVD).. We investigated the effects of AR extracts on rat cardiomyocytes and H9C2 cardiac cells as well as identified many target genes that mediate the effect of AR.. Materials and methods: The effect of AR extracts on cell proliferation was assessed and cDNA microarray technique was used to analyse the differential gene expressions upon AR treatment in cardiac cells.

Purification:

Article Title: SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function.
Article Snippet: Fontijn RD, Volger OL, Fledderus JO, Reijerkerk A, de Vries HE, Horrevoets AJ.. SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function.. Am J Physiol Heart Circ Physiol 294: H891–H900, 2008.



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