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TransGen biotech co transscripttm reverse transcriptase
Transscripttm Reverse Transcriptase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transscripttm+reverse+transcriptase/transscript+reverse+transcriptase/pm23816956-75-38-41
Average 90 stars, based on 1 article reviews
transscripttm reverse transcriptase - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Quantitative RT-PCR:

Article Title: Long Non-Coding RNA HOTAIR Promotes Human Osteosarcoma Proliferation, Migration through Activation of the Wnt/b-Catenin Signaling Pathway
Article Snippet: The TRIzol reagent was used to extract complete RNA (Sangon Biotech, Shanghai, China). .. For the qRT-PCR experiment, 2 μ g of complete RNA for reverse transcript and cDNA synthesis is used with TransScript ® II Reverse Transcriptase (TransGen Biotech Co,, Beijing, China). .. Quantitative, real-time PCR experiments have been carried out with PerfectStartTM Green qPCR SuperMix (TransGen Biotech Co., Beijing, China).

cDNA Synthesis:

Article Title: Long Non-Coding RNA HOTAIR Promotes Human Osteosarcoma Proliferation, Migration through Activation of the Wnt/b-Catenin Signaling Pathway
Article Snippet: The TRIzol reagent was used to extract complete RNA (Sangon Biotech, Shanghai, China). .. For the qRT-PCR experiment, 2 μ g of complete RNA for reverse transcript and cDNA synthesis is used with TransScript ® II Reverse Transcriptase (TransGen Biotech Co,, Beijing, China). .. Quantitative, real-time PCR experiments have been carried out with PerfectStartTM Green qPCR SuperMix (TransGen Biotech Co., Beijing, China).

Article Title: Enolase, a cadmium resistance related protein from hyperaccumulator plant Phytolacca americana , increase the tolerance of Escherichia coli to cadmium stress.
Article Snippet: Phytolacca americana is a Cd hyperaccumulator plant that accumulates significant amounts of Cd in leaves, making it a valuable phytoremediation plant species.. Our previous research found enolase (ENO) may play an important part in P. americana to cope with Cd stress.. As a multifunctional enzyme, ENO was involved not only in glycolysis but also in the response of plants to various environmental stresses.

Article Title: Phenotype and molecular characterizations of a family with dentinogenesis imperfecta shields type II with a novel DSPP mutation
Article Snippet: .. Total RNA (100 ng) from each sample was extracted using TRIzol (TransGen ® , China) and was used for cDNA synthesis using the TransScript Reverse Transcription System (TransGen Biotech ® , Beijing, China). .. We then used qRT-PCR to measure the messenger RNA (mRNA) levels of DSPP , ALP , and RUNX2 using ChamQTM Universal SYBR Qpcr Master Mix (Vazyme ® , Nanjing, China).

Reverse Transcription:

Article Title: Long Non-Coding RNA HOTAIR Promotes Human Osteosarcoma Proliferation, Migration through Activation of the Wnt/b-Catenin Signaling Pathway
Article Snippet: The TRIzol reagent was used to extract complete RNA (Sangon Biotech, Shanghai, China). .. For the qRT-PCR experiment, 2 μ g of complete RNA for reverse transcript and cDNA synthesis is used with TransScript ® II Reverse Transcriptase (TransGen Biotech Co,, Beijing, China). .. Quantitative, real-time PCR experiments have been carried out with PerfectStartTM Green qPCR SuperMix (TransGen Biotech Co., Beijing, China).

Article Title: Small kernel 501 (smk501) encodes the RUBylation activating enzyme E1 subunit ECR1 (E1 C-TERMINAL RELATED 1) and is essential for multiple aspects of cellular events during kernel development in maize.
Article Snippet: RUBylation plays essential roles in plant growth and development through regulating Cullin-RING ubiquitin E3 ligase (CRL) activities and the CRL-mediated protein degradations.. However, the function of RUBylation in regulating kernel development remains unclear.. Through genetic and molecular analyses of a small kernel 501 (smk501) mutant in maize (Zea mays), we cloned the smk501 gene, revealed its molecular function, and defined its roles in RUBylation pathway and seed development.

Article Title: iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis.
Article Snippet: Currently, there remains a great need to elucidate the molecular mechanism of acute myocardial infarction in order to facilitate the development of novel therapy.. Inhibitor of apoptosis-stimulating protein of p53 (iASPP) is a member of the ASPP family proteins and an evolutionarily preserved inhibitor of p53 that is involved in many cellular processes, including apoptosis of cancer cells.. The purpose of this study was to investigate the possible role of iASPP in acute myocardial infarction.

Article Title: Enolase, a cadmium resistance related protein from hyperaccumulator plant Phytolacca americana , increase the tolerance of Escherichia coli to cadmium stress.
Article Snippet: Phytolacca americana is a Cd hyperaccumulator plant that accumulates significant amounts of Cd in leaves, making it a valuable phytoremediation plant species.. Our previous research found enolase (ENO) may play an important part in P. americana to cope with Cd stress.. As a multifunctional enzyme, ENO was involved not only in glycolysis but also in the response of plants to various environmental stresses.

Article Title: Phenotype and molecular characterizations of a family with dentinogenesis imperfecta shields type II with a novel DSPP mutation
Article Snippet: .. Total RNA (100 ng) from each sample was extracted using TRIzol (TransGen ® , China) and was used for cDNA synthesis using the TransScript Reverse Transcription System (TransGen Biotech ® , Beijing, China). .. We then used qRT-PCR to measure the messenger RNA (mRNA) levels of DSPP , ALP , and RUNX2 using ChamQTM Universal SYBR Qpcr Master Mix (Vazyme ® , Nanjing, China).

Article Title: Sequence-specific nanoparticle barcode strategy for multiplex human enterovirus typing
Article Snippet: .. PCR was performed in a 25 μL reaction system, which contained 50 mM Tris-HCl (pH 8.5), 2 mM MgSO4, 70 mM CH3COOK, 200 μM dNTPs, 2 U Taq HS DNA polymerase (TaKaRa), 4 U TransScript Reverse Transcriptase (TransGen Biotech), 0.4 μM of universal primers, and 5 μL of template. .. Thermal cycling consisted of the reverse transcription step at 50 °C for 10 min, an enzyme activation step at 95 °C for 5 min, followed by thirty cycles of 95 °C for 15 s, and 55 °C for 45 s. PCR was performed on a T100 thermal cycler (Bio-Rad).

Article Title: Block and Boost DNA Transfer: Opposite Roles of OmpA in Natural and Artificial Transformation of Escherichia coli
Article Snippet: .. RNA from the FS20-pSU (carrying rpoS null mutation), FS20-pSURpoS (RpoS over-expressing strain) was isolated and reverse transcribed to cDNA with TransScript Reverse Transcriptase (TransGen Biotech). ..

Real-time Polymerase Chain Reaction:

Article Title: Small kernel 501 (smk501) encodes the RUBylation activating enzyme E1 subunit ECR1 (E1 C-TERMINAL RELATED 1) and is essential for multiple aspects of cellular events during kernel development in maize.
Article Snippet: RUBylation plays essential roles in plant growth and development through regulating Cullin-RING ubiquitin E3 ligase (CRL) activities and the CRL-mediated protein degradations.. However, the function of RUBylation in regulating kernel development remains unclear.. Through genetic and molecular analyses of a small kernel 501 (smk501) mutant in maize (Zea mays), we cloned the smk501 gene, revealed its molecular function, and defined its roles in RUBylation pathway and seed development.

In Situ Hybridization:

Article Title: Small kernel 501 (smk501) encodes the RUBylation activating enzyme E1 subunit ECR1 (E1 C-TERMINAL RELATED 1) and is essential for multiple aspects of cellular events during kernel development in maize.
Article Snippet: RUBylation plays essential roles in plant growth and development through regulating Cullin-RING ubiquitin E3 ligase (CRL) activities and the CRL-mediated protein degradations.. However, the function of RUBylation in regulating kernel development remains unclear.. Through genetic and molecular analyses of a small kernel 501 (smk501) mutant in maize (Zea mays), we cloned the smk501 gene, revealed its molecular function, and defined its roles in RUBylation pathway and seed development.

RNA Extraction:

Article Title: Enolase, a cadmium resistance related protein from hyperaccumulator plant Phytolacca americana , increase the tolerance of Escherichia coli to cadmium stress.
Article Snippet: Phytolacca americana is a Cd hyperaccumulator plant that accumulates significant amounts of Cd in leaves, making it a valuable phytoremediation plant species.. Our previous research found enolase (ENO) may play an important part in P. americana to cope with Cd stress.. As a multifunctional enzyme, ENO was involved not only in glycolysis but also in the response of plants to various environmental stresses.

Isolation:

Article Title: Enolase, a cadmium resistance related protein from hyperaccumulator plant Phytolacca americana , increase the tolerance of Escherichia coli to cadmium stress.
Article Snippet: Phytolacca americana is a Cd hyperaccumulator plant that accumulates significant amounts of Cd in leaves, making it a valuable phytoremediation plant species.. Our previous research found enolase (ENO) may play an important part in P. americana to cope with Cd stress.. As a multifunctional enzyme, ENO was involved not only in glycolysis but also in the response of plants to various environmental stresses.

Article Title: Block and Boost DNA Transfer: Opposite Roles of OmpA in Natural and Artificial Transformation of Escherichia coli
Article Snippet: .. RNA from the FS20-pSU (carrying rpoS null mutation), FS20-pSURpoS (RpoS over-expressing strain) was isolated and reverse transcribed to cDNA with TransScript Reverse Transcriptase (TransGen Biotech). ..

Synthesized:

Article Title: Enolase, a cadmium resistance related protein from hyperaccumulator plant Phytolacca americana , increase the tolerance of Escherichia coli to cadmium stress.
Article Snippet: Phytolacca americana is a Cd hyperaccumulator plant that accumulates significant amounts of Cd in leaves, making it a valuable phytoremediation plant species.. Our previous research found enolase (ENO) may play an important part in P. americana to cope with Cd stress.. As a multifunctional enzyme, ENO was involved not only in glycolysis but also in the response of plants to various environmental stresses.

Polymerase Chain Reaction:

Article Title: Sequence-specific nanoparticle barcode strategy for multiplex human enterovirus typing
Article Snippet: .. PCR was performed in a 25 μL reaction system, which contained 50 mM Tris-HCl (pH 8.5), 2 mM MgSO4, 70 mM CH3COOK, 200 μM dNTPs, 2 U Taq HS DNA polymerase (TaKaRa), 4 U TransScript Reverse Transcriptase (TransGen Biotech), 0.4 μM of universal primers, and 5 μL of template. .. Thermal cycling consisted of the reverse transcription step at 50 °C for 10 min, an enzyme activation step at 95 °C for 5 min, followed by thirty cycles of 95 °C for 15 s, and 55 °C for 45 s. PCR was performed on a T100 thermal cycler (Bio-Rad).

Mutagenesis:

Article Title: Block and Boost DNA Transfer: Opposite Roles of OmpA in Natural and Artificial Transformation of Escherichia coli
Article Snippet: .. RNA from the FS20-pSU (carrying rpoS null mutation), FS20-pSURpoS (RpoS over-expressing strain) was isolated and reverse transcribed to cDNA with TransScript Reverse Transcriptase (TransGen Biotech). ..

other:

Article Title: Engineering for an HPV 9-valent Vaccine using Genomic Constitutive Over-expression and Low Lipopolysaccharide Levels in Escherichia Coli Cells
Article Snippet: One-step qPCR was performed using the listed primers (16sRNA F: 5’-TGATAAACTGGAGGAAGGTG-3’ R:5’- CACTTTATGAGGTCCGCTTG-3’, HPV16L1 F: 5’- GTCCCAGTATCTAAGGTTGT-3’, R 5’-GGTTTTTTAATAGGAAAATA -3’) and probes (16sRNA, 5’- TGGCCCTTACGACCAGGGCT-3’, HPV16L1, 5’-TCATGCAGGAACATCCAGAC-3’ ) with the following reaction volumes: 1 μl template, 0.5 μl F/R primers, 64 μl of 10× buffer, 2 μl of 2.5 mM dNTP, 0.4 μl HS Taq, 0.2 μl TransScript II Reverse Transcriptase (TransGen Biotech, Beijing), 10.4 μl DEPC H2O, and 1 μl probe.



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