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Beijing TransGen Biotech transscripttm reverse transcriptase
Transscripttm Reverse Transcriptase, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transscripttm+reverse+transcriptase/transscript+reverse+transcriptase/pm31257543-24-16-19
Average 90 stars, based on 1 article reviews
transscripttm reverse transcriptase - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: Aminoguanidine inhibits IL-1β-induced protein expression of iNOS and COX-2 by blocking the NF-κB signaling pathway in rat articular chondrocytes
Article Snippet: Total RNA was extracted from the chondrocyte cells using the BioSciTM Tissue/Cultured Cell Total RNA Extraction kit (Dakewe Bioengineering Co., Ltd.), according to the manufacturer's instructions. .. Total RNA (1 μg), dNTPs (cat. no. AD101-12; Beijing TransGen Biotech Co., Ltd.), primer (cat. no. AH101-02; Beijing TransGen Biotech Co., Ltd.) and TransScript ® II reverse transcriptase (cat. no. AH101-02; Beijing TransGen Biotech Co., Ltd.) were used to synthesize cDNA. .. The temperature protocol was as follows: 50 ̊C for 30 min and 85 ̊C for 5 sec. Quantitative PCR (qPCR) was performed using SuperReal PreMix Plus (SYBR Green; cat. no. FP205; Tiangen Biotech Co., Ltd.) according to the manufacturer's protocols.


Article Title: Chlorogenic acid improves food allergy through the AMPK/ACC/CPT-1 pathway.
Article Snippet: Ultrapure RNA extraction reagent was purchased from TaKaRa Bio Inc (Kyoto, Japan). .. Reverse transcription TransScript II Reverse, qPCR kit was purchased from Beijing TransGen Biotech Co, Ltd (Haidian District, Beijing, China). .. Real- time PCR primers were synthesized by Beijing Liuhe Huada Gene Technology Co, Ltd (Daxing District, Beijing, China).

Article Title: Ginsenosides regulate adventitious root formation in Panax ginseng via a CLE45-WOX11 regulatory module.
Article Snippet: Adventitious root branching is vital to plant growth and regeneration, but the regulation of this process remains unclear.. We therefore investigated how ginsenosides regulate adventitious root branching in Panax ginseng.. Cell proliferation and adventitious root branching were decreased in the presence of ginsenoside Rb1 and a high concentration of ginsenoside Re, but increased when treating with a low concentration of Re.

Article Title: Identification and 3D gene expression patterns of WUSCEHEL-related homeobox (WOX) genes from Panax ginseng.
Article Snippet: Wild ginseng (Panax ginseng) can survive in their natural habitat for hundreds of years, reflecting a remarkable plasticity.. Plant stem cells (SCs) play a key role in the regenerative capacity and lifelong activity of these plants.. WUSCHEL-RELATED HOMEOBOX (WOX) genes are master regulators of plant SC pluripotency, but their functions in medicinal plants have not been previously reported.

Synthesized:


Quantitative RT-PCR:


other:

Article Title: Aminoguanidine inhibits IL-1β-induced protein expression of iNOS and COX-2 by blocking the NF-κB signaling pathway in rat articular chondrocytes.
Article Snippet: Total RNA (1 μg), dNTPs (cat. no. AD101-12; Beijing TransGen Biotech Co., Ltd.), primer (cat. no. AH101-02; Beijing TransGen Biotech Co., Ltd.) and TransScript®II reverse transcriptase (cat. no. AH101-02; Beijing TransGen Biotech Co., Ltd.) were used to synthesize cDNA.

Article Title: Thiram induces myocardial oxidative damage and apoptosis in broilers via interfering their cardiac metabolism.
Article Snippet: The reverse transcriptase process (Beijing TransGen Biotech) was used to produce single-strand cDNAs for real-time PCR (qRT-PCR) detection.

Real-time Polymerase Chain Reaction:

Article Title: Chlorogenic acid improves food allergy through the AMPK/ACC/CPT-1 pathway.
Article Snippet: Ultrapure RNA extraction reagent was purchased from TaKaRa Bio Inc (Kyoto, Japan). .. Reverse transcription TransScript II Reverse, qPCR kit was purchased from Beijing TransGen Biotech Co, Ltd (Haidian District, Beijing, China). .. Real- time PCR primers were synthesized by Beijing Liuhe Huada Gene Technology Co, Ltd (Daxing District, Beijing, China).

Concentration Assay:

Article Title: Identification and 3D gene expression patterns of WUSCEHEL-related homeobox (WOX) genes from Panax ginseng.
Article Snippet: Wild ginseng (Panax ginseng) can survive in their natural habitat for hundreds of years, reflecting a remarkable plasticity.. Plant stem cells (SCs) play a key role in the regenerative capacity and lifelong activity of these plants.. WUSCHEL-RELATED HOMEOBOX (WOX) genes are master regulators of plant SC pluripotency, but their functions in medicinal plants have not been previously reported.

Spectrophotometry:

Article Title: Identification and 3D gene expression patterns of WUSCEHEL-related homeobox (WOX) genes from Panax ginseng.
Article Snippet: Wild ginseng (Panax ginseng) can survive in their natural habitat for hundreds of years, reflecting a remarkable plasticity.. Plant stem cells (SCs) play a key role in the regenerative capacity and lifelong activity of these plants.. WUSCHEL-RELATED HOMEOBOX (WOX) genes are master regulators of plant SC pluripotency, but their functions in medicinal plants have not been previously reported.



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