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primescript reverse transcriptase kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript reverse transcriptase kit
    Primescript Reverse Transcriptase Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase/PrimeScript+Reverse+Transcriptase/pm42883128-70-12-16
    Average 97 stars, based on 4949 article reviews
    primescript reverse transcriptase kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: CX3CL1–CX3CR1 axis promotes restraint stress-induced thymic involution through macrophage-mediated thymocyte apoptosis
    Article Snippet: .. Total RNAs were reverse transcribed to complementary DNA (cDNA) using Prime Script Reverse Transcriptase (Takara Bio, Shiga, Japan) with oligo(dT) 15 primers. .. PCR was carried out using SYBR Premix Ex Taq II (Takara Bio) with specific primer sets (Takara Bio, ).

    Article Title: lncRNA-5583 Modulates Porcine Granulosa Cell Proliferation Through a miR-193a-5p/ACVR1 -Associated Mechanism.
    Article Snippet: .. A total of 500 ng RNA was reverse-transcribed into cDNA using the PrimeScript Reverse Transcriptase Kit (Takara Bio Inc., Kusatsu, Shiga, Japan). .. RT-qPCR was performed using https://doi.org/10.3390/ani16193159 ChamQ Universal SYBR qPCR Master Mix (Nanjing Vazyme Biotech Co., Ltd., Nanjing, China) on an FQD-96A Touch Real-Time PCR System (Hangzhou Bioer Technology Co., Ltd., Hangzhou, China) in a total reaction volume of 20 μL.

    Article Title: Oxidative stress drives disuse-induced chronic pain through sex-divergent immune-neural mechanisms.
    Article Snippet: Physical disuse is a major risk factor for complex regional pain syndrome type I.. Using a rat chronic post-cast pain model, we investigated how oxidative stress modulates pain processing across the neuraxis and evaluated sex as a biological variable.. Following immobilization, both sexes developed widespread mechanical hyperalgesia, which was markedly attenuated by the antioxidant Tempol.

    Article Title: Lactiplantibacillus plantarum HOKKAIDO strain drives alveolar macrophage-dependent antiviral protection against respiratory infection.
    Article Snippet: Total RNA was extracted from the spleen cells using the Direct-zol RNA Miniprep Plus (Zyomo Research, Irvine, CA, USA) and purified using the Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturers’ instructions. .. cDNA was synthesized using PrimeScript Reverse Transcriptase (Takara Bio, Shiga, Japan) with an Oligo dT primer, following the manufacturer’s instructions. ..

    Article Title: Quiescence to proliferation transition regulates asynchronous mitochondrial DNA replication dynamics.
    Article Snippet: Total RNA was extracted from cells using the RNAiso Plus reagent (TaKaRa). .. Puried RNA was treated with DNase I at 37 °C for 30 min. cDNA was reverse- transcribed by PrimeScript reverse transcriptase (TaKaRa) and analyzed by qPCR using TB Green Premix Ex Taq II (TaKaRa) in a 25 μL reaction solution. ..

    Article Title: Repurposing approved drugs as Pendrin ( SLC26A4 ) inhibitors in allergic asthma: single-cell nomination, structure-based screening and functional validation
    Article Snippet: .. Total RNA was extracted with TRIzol reagent and reverse-transcribed with PrimeScript reverse transcriptase (Takara). ..

    Article Title: Multi-omic profiling defines subtype-specific extracellular vesicle signatures in early human pregnancy.
    Article Snippet: Small RNA libraries were prepared using a modified SMARTer smRNA-Seq Kit for Illumina (Takara Bio USA, San Jose, CA) optimized for low-input small RNA species including miRNA, small interfering RNA (siRNA), PIWI-interacting RNA (piRNA), transfer RNA (tRNA), and small nucleolar RNA (snoRNA). .. RNA from EVs was polyadenylated and reverse transcribed into cDNA using PrimeScript RT (Takara Bio USA, San Jose, CA) with template switching to incorporate adapter sequences. ..

    Synthesized:

    Article Title: Lactiplantibacillus plantarum HOKKAIDO strain drives alveolar macrophage-dependent antiviral protection against respiratory infection.
    Article Snippet: Total RNA was extracted from the spleen cells using the Direct-zol RNA Miniprep Plus (Zyomo Research, Irvine, CA, USA) and purified using the Monarch RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturers’ instructions. .. cDNA was synthesized using PrimeScript Reverse Transcriptase (Takara Bio, Shiga, Japan) with an Oligo dT primer, following the manufacturer’s instructions. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Quiescence to proliferation transition regulates asynchronous mitochondrial DNA replication dynamics.
    Article Snippet: Total RNA was extracted from cells using the RNAiso Plus reagent (TaKaRa). .. Puried RNA was treated with DNase I at 37 °C for 30 min. cDNA was reverse- transcribed by PrimeScript reverse transcriptase (TaKaRa) and analyzed by qPCR using TB Green Premix Ex Taq II (TaKaRa) in a 25 μL reaction solution. ..



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