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data rnaseq data arrayexpress database arrayexpress e mtab 12645 experimental models
Data Rnaseq Data Arrayexpress Database Arrayexpress E Mtab 12645 Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+arrayexpress+database/Streptomyces+sp%2E+Waksman+and+Henrici/pm36898381-949-181-202
Average 93 stars, based on 20 article reviews
Data Rnaseq Data Arrayexpress Database Arrayexpress E Mtab 12645 Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+arrayexpress+database/Streptomyces+sp%2E+Waksman+and+Henrici/pm36898381-949-181-202
Average 93 stars, based on 20 article reviews
data rnaseq data arrayexpress database arrayexpress e mtab 12645 experimental models - by Bioz Stars,
2026-09
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other:Article Title: Soybean variety 01073059 Article Snippet: The date of deposit is Dec. 3, 2019 and the accession number for those deposited seeds of soybean variety 01073059 is Article Title: Proposal to reclassify the proteobacterial classes Deltaproteobacteria and Oligoflexia , and the phylum Thermodesulfobacteria into four phyla reflecting major functional capabilities. Article Snippet: The type strain is Plasmid Preparation:Article Title: gEL DNA: A Cloning- and Polymerase Chain Reaction–Free Method for CRISPR-Based Multiplexed Genome Editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Fn Cas12a-crRNAs, was constructed by Golden Gate assembly of pre-annealed primers 12647–12648 with the following PCR generated fragments: the pGGKd018 backbone with primers 12799–12800; the SNR52 promoter amplified from the pMEL13 template 4 using Construct:Article Title: gEL DNA: A Cloning- and Polymerase Chain Reaction–Free Method for CRISPR-Based Multiplexed Genome Editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Fn Cas12a-crRNAs, was constructed by Golden Gate assembly of pre-annealed primers 12647–12648 with the following PCR generated fragments: the pGGKd018 backbone with primers 12799–12800; the SNR52 promoter amplified from the pMEL13 template 4 using Article Title: gEL DNA, a cloning- and PCR-free method for CRISPR-based multiplexed genome editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Cas12a-crRNAs, was constructed by Golden Gate assembly of Polymerase Chain Reaction:Article Title: gEL DNA: A Cloning- and Polymerase Chain Reaction–Free Method for CRISPR-Based Multiplexed Genome Editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Fn Cas12a-crRNAs, was constructed by Golden Gate assembly of pre-annealed primers 12647–12648 with the following PCR generated fragments: the pGGKd018 backbone with primers 12799–12800; the SNR52 promoter amplified from the pMEL13 template 4 using Article Title: gEL DNA, a cloning- and PCR-free method for CRISPR-based multiplexed genome editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Cas12a-crRNAs, was constructed by Golden Gate assembly of Generated:Article Title: gEL DNA: A Cloning- and Polymerase Chain Reaction–Free Method for CRISPR-Based Multiplexed Genome Editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Fn Cas12a-crRNAs, was constructed by Golden Gate assembly of pre-annealed primers 12647–12648 with the following PCR generated fragments: the pGGKd018 backbone with primers 12799–12800; the SNR52 promoter amplified from the pMEL13 template 4 using Article Title: gEL DNA, a cloning- and PCR-free method for CRISPR-based multiplexed genome editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Cas12a-crRNAs, was constructed by Golden Gate assembly of Amplification:Article Title: gEL DNA: A Cloning- and Polymerase Chain Reaction–Free Method for CRISPR-Based Multiplexed Genome Editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Fn Cas12a-crRNAs, was constructed by Golden Gate assembly of pre-annealed primers 12647–12648 with the following PCR generated fragments: the pGGKd018 backbone with primers 12799–12800; the SNR52 promoter amplified from the pMEL13 template 4 using Article Title: gEL DNA, a cloning- and PCR-free method for CRISPR-based multiplexed genome editing Article Snippet: .. The GFPdo pUDE810, an entry vector for Cas12a-crRNAs, was constructed by Golden Gate assembly of Recombinant:Article Title: Linoleic acid potentiates CD8 + T cell metabolic fitness and antitumor immunity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-mouse TCF1 - APC BD Biosciences CAT#566693, RRID: AB_2869823 anti-mouse TCR Vb5.2, 5.2 - PE Biolegend CAT#139504, RRID: AB_10613279 anti-mouse TCR Vb5.2, 5.2 - FITC Biolegend CAT#139514, RRID: AB_2750133 anti-mouse TIM3 - BV786 BD Biosciences CAT#747621, RRID: AB_2744187 anti-mouse TNF - BV650 BD Biosciences CAT#563943, RRID: AB_2738498 anti-mouse/human TOX - APC Miltenyi Biotec CAT#130-118-335, RRID: AB_2751485 CFSE BD Biosciences CAT#565082, RRID: AB_2869649 CD3 Monoclonal Antibody (OKT3) Invitrogen CAT#16-0037-81, RRID: AB_468854 Cleaved Caspase-3 (Asp175) Cell signaling CAT#9661, RRID: AB_2069869 Far-Red Fluorescent Live-Cell Impermeant DNA Dye DRAQ7 BioStatus Limited DR71000 Fixable Viability Dye 640/662 Biotum 32007A Fixable viability Stain 510 BD Biosciences CAT#564406, RRID: AB_2869572 GRP78 Invitrogen CAT#PA1014A, RRID: AB_559362 Histone H3 Abcam CAT#ab1791, RRID: AB_302613 OXPHOS Abcam CAT#ab110413, RRID: AB_2629281 Phospho-s6 Ser240/244 Cell signaling CAT#5364, RRID: AB_10694233 Purified anti-human Cd28 BD Biosciences CAT#556620, RRID: AB_396492 Purified anti-human Cd3 BD Biosciences CAT#551916, RRID: AB_394293 Purified anti-mouse Cd28 BD Biosciences CAT#553294, RRID: AB_394763 Purified anti-mouse Cd3 BD Biosciences CAT#553057, RRID: AB_394590 Alexa488-affini Donkey Anti-rabbit IgG Invitrogen CAT#A-21206, RRID: AB_2535792 VDAC (D73D12) Cell signaling CAT#4661, RRID: AB_10557420 Vinculin Sigma CAT#V9131, RRID: AB_477629 APC Goat anti-Mouse IgG Invitrogen CAT#A-865, RRID: AB_141364 Chemical, peptides, and recombinant proteins 2-mercaptoethanol Invitrogen 31350010-50mM 4 Diamidino 2 Phenylindole Sigma 32670-25MG-F 4-phenylbutyric Acid Sigma P21005 ACK Lysing buffer Invitrogen A10492 Albumin Bovine Fraction Sigma A8806 Approved Fbs South American EuroClone SpA ECS0165L Arachidonic Acid- BSA conjugated Aurogene OOCD00263 Calcein AM Life technologies C3100MP Colegenase from C. histolyticum Sigma C2674-1G CTS OpTmizer T Cell medium Invitrogen A1048501 D-( )-glucose Solution Sigma G8769 Deoxyribonuclease I Sigma D5025-375KU Etomoxir Sigma E1905-5MG Ficoll Paque Plus Sigma GE17-1440-03 GolgiPlug BD Biosciences 555029 Hcs Lipidtox Red Neutral Lipid Stain Life technologies H34476 Hoechst No. 33342 Sigma B2261 human AB serum AnProtec ACSM0071 Ionomycin Sigma I0634 Linoleic Acid - BSA conjugated Sigma L9530 MitoTracker Green FM Invitrogen M7514 MitoTracker Orange CMTMRos Invitrogen M7510 Nocodazole Sigma M1404 (Continued on next page) e2 Cell Metabolism 35, 633–650.e1–e9, April 4, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oleic Acid - BSA conjugated Sigma O3008 OVA peptide (SIINFEKL) SB-Peptide N/A Paraformaldehyde Invitrogen 043368.9L Penicillin/Streptomicin 100X EuroClone SpA ECB3001D PMA Sigma P1585 Recombinant human Interleukin 15 Miltenyi 130-095-762 Recombinant human Interleukin 2 Novartis CLB-P-476-800-13980 IT Recombinant human Interleukin 7 Miltenyi 130-095-361 Rpmi 1640 With Stable L-glutamine EuroClone SpA ECM2001L Seahorse Xf Ph 7.4 Rpmi Agilent 103681-100 Sodium Pyruvate Solution 100 Nm EuroClone SpA LOBE13115E Thapsigargin Sigma T9033 Triton X100 Vwr International 1.08603.1000 U-13C18 Linoleic acid Cambridge Isotopes CLM-6855 Critical commercial assays Acetyl-Coenzyme A Assay Kit Sigma MAK039 Atp 1 Determination Kit Invitrogen A22066 BCA Protein Assay kit ThermoScientific 23227 CD8+ T cell isolation Kit Human Miltenyi 130-096-495 CD8+ T cell isolation Kit Mouse Miltenyi 130-104-075 Clarity Western ECL substrate Biorad 170-5061 Foxp3 Fix/perm Buffer Set BD Biosciences 560409 Lactate Assay Kit Sigma MAK064 LS columns Miltenyi 130-042-401 RetroNectin Takara-Bio T110A Rneasy Minielute Cleanup Kit Qiagen 74204 Seahorse Xf Cell Mito Stress Test Kit Agilent 103015-100 Seahorse Xf Glycolytic Rate Test Kit Agilent 103344-100 Seahorse Xfe96 Fluxpak Mini Agilent 102601-100 Ultracomp Ebeads Affymetrix Invitrogen 01-2222-42 Deposited Bicinchoninic Acid Protein Assay:Article Title: Linoleic acid potentiates CD8 + T cell metabolic fitness and antitumor immunity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-mouse TCF1 - APC BD Biosciences CAT#566693, RRID: AB_2869823 anti-mouse TCR Vb5.2, 5.2 - PE Biolegend CAT#139504, RRID: AB_10613279 anti-mouse TCR Vb5.2, 5.2 - FITC Biolegend CAT#139514, RRID: AB_2750133 anti-mouse TIM3 - BV786 BD Biosciences CAT#747621, RRID: AB_2744187 anti-mouse TNF - BV650 BD Biosciences CAT#563943, RRID: AB_2738498 anti-mouse/human TOX - APC Miltenyi Biotec CAT#130-118-335, RRID: AB_2751485 CFSE BD Biosciences CAT#565082, RRID: AB_2869649 CD3 Monoclonal Antibody (OKT3) Invitrogen CAT#16-0037-81, RRID: AB_468854 Cleaved Caspase-3 (Asp175) Cell signaling CAT#9661, RRID: AB_2069869 Far-Red Fluorescent Live-Cell Impermeant DNA Dye DRAQ7 BioStatus Limited DR71000 Fixable Viability Dye 640/662 Biotum 32007A Fixable viability Stain 510 BD Biosciences CAT#564406, RRID: AB_2869572 GRP78 Invitrogen CAT#PA1014A, RRID: AB_559362 Histone H3 Abcam CAT#ab1791, RRID: AB_302613 OXPHOS Abcam CAT#ab110413, RRID: AB_2629281 Phospho-s6 Ser240/244 Cell signaling CAT#5364, RRID: AB_10694233 Purified anti-human Cd28 BD Biosciences CAT#556620, RRID: AB_396492 Purified anti-human Cd3 BD Biosciences CAT#551916, RRID: AB_394293 Purified anti-mouse Cd28 BD Biosciences CAT#553294, RRID: AB_394763 Purified anti-mouse Cd3 BD Biosciences CAT#553057, RRID: AB_394590 Alexa488-affini Donkey Anti-rabbit IgG Invitrogen CAT#A-21206, RRID: AB_2535792 VDAC (D73D12) Cell signaling CAT#4661, RRID: AB_10557420 Vinculin Sigma CAT#V9131, RRID: AB_477629 APC Goat anti-Mouse IgG Invitrogen CAT#A-865, RRID: AB_141364 Chemical, peptides, and recombinant proteins 2-mercaptoethanol Invitrogen 31350010-50mM 4 Diamidino 2 Phenylindole Sigma 32670-25MG-F 4-phenylbutyric Acid Sigma P21005 ACK Lysing buffer Invitrogen A10492 Albumin Bovine Fraction Sigma A8806 Approved Fbs South American EuroClone SpA ECS0165L Arachidonic Acid- BSA conjugated Aurogene OOCD00263 Calcein AM Life technologies C3100MP Colegenase from C. histolyticum Sigma C2674-1G CTS OpTmizer T Cell medium Invitrogen A1048501 D-( )-glucose Solution Sigma G8769 Deoxyribonuclease I Sigma D5025-375KU Etomoxir Sigma E1905-5MG Ficoll Paque Plus Sigma GE17-1440-03 GolgiPlug BD Biosciences 555029 Hcs Lipidtox Red Neutral Lipid Stain Life technologies H34476 Hoechst No. 33342 Sigma B2261 human AB serum AnProtec ACSM0071 Ionomycin Sigma I0634 Linoleic Acid - BSA conjugated Sigma L9530 MitoTracker Green FM Invitrogen M7514 MitoTracker Orange CMTMRos Invitrogen M7510 Nocodazole Sigma M1404 (Continued on next page) e2 Cell Metabolism 35, 633–650.e1–e9, April 4, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oleic Acid - BSA conjugated Sigma O3008 OVA peptide (SIINFEKL) SB-Peptide N/A Paraformaldehyde Invitrogen 043368.9L Penicillin/Streptomicin 100X EuroClone SpA ECB3001D PMA Sigma P1585 Recombinant human Interleukin 15 Miltenyi 130-095-762 Recombinant human Interleukin 2 Novartis CLB-P-476-800-13980 IT Recombinant human Interleukin 7 Miltenyi 130-095-361 Rpmi 1640 With Stable L-glutamine EuroClone SpA ECM2001L Seahorse Xf Ph 7.4 Rpmi Agilent 103681-100 Sodium Pyruvate Solution 100 Nm EuroClone SpA LOBE13115E Thapsigargin Sigma T9033 Triton X100 Vwr International 1.08603.1000 U-13C18 Linoleic acid Cambridge Isotopes CLM-6855 Critical commercial assays Acetyl-Coenzyme A Assay Kit Sigma MAK039 Atp 1 Determination Kit Invitrogen A22066 BCA Protein Assay kit ThermoScientific 23227 CD8+ T cell isolation Kit Human Miltenyi 130-096-495 CD8+ T cell isolation Kit Mouse Miltenyi 130-104-075 Clarity Western ECL substrate Biorad 170-5061 Foxp3 Fix/perm Buffer Set BD Biosciences 560409 Lactate Assay Kit Sigma MAK064 LS columns Miltenyi 130-042-401 RetroNectin Takara-Bio T110A Rneasy Minielute Cleanup Kit Qiagen 74204 Seahorse Xf Cell Mito Stress Test Kit Agilent 103015-100 Seahorse Xf Glycolytic Rate Test Kit Agilent 103344-100 Seahorse Xfe96 Fluxpak Mini Agilent 102601-100 Ultracomp Ebeads Affymetrix Invitrogen 01-2222-42 Deposited Cell Isolation:Article Title: Linoleic acid potentiates CD8 + T cell metabolic fitness and antitumor immunity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-mouse TCF1 - APC BD Biosciences CAT#566693, RRID: AB_2869823 anti-mouse TCR Vb5.2, 5.2 - PE Biolegend CAT#139504, RRID: AB_10613279 anti-mouse TCR Vb5.2, 5.2 - FITC Biolegend CAT#139514, RRID: AB_2750133 anti-mouse TIM3 - BV786 BD Biosciences CAT#747621, RRID: AB_2744187 anti-mouse TNF - BV650 BD Biosciences CAT#563943, RRID: AB_2738498 anti-mouse/human TOX - APC Miltenyi Biotec CAT#130-118-335, RRID: AB_2751485 CFSE BD Biosciences CAT#565082, RRID: AB_2869649 CD3 Monoclonal Antibody (OKT3) Invitrogen CAT#16-0037-81, RRID: AB_468854 Cleaved Caspase-3 (Asp175) Cell signaling CAT#9661, RRID: AB_2069869 Far-Red Fluorescent Live-Cell Impermeant DNA Dye DRAQ7 BioStatus Limited DR71000 Fixable Viability Dye 640/662 Biotum 32007A Fixable viability Stain 510 BD Biosciences CAT#564406, RRID: AB_2869572 GRP78 Invitrogen CAT#PA1014A, RRID: AB_559362 Histone H3 Abcam CAT#ab1791, RRID: AB_302613 OXPHOS Abcam CAT#ab110413, RRID: AB_2629281 Phospho-s6 Ser240/244 Cell signaling CAT#5364, RRID: AB_10694233 Purified anti-human Cd28 BD Biosciences CAT#556620, RRID: AB_396492 Purified anti-human Cd3 BD Biosciences CAT#551916, RRID: AB_394293 Purified anti-mouse Cd28 BD Biosciences CAT#553294, RRID: AB_394763 Purified anti-mouse Cd3 BD Biosciences CAT#553057, RRID: AB_394590 Alexa488-affini Donkey Anti-rabbit IgG Invitrogen CAT#A-21206, RRID: AB_2535792 VDAC (D73D12) Cell signaling CAT#4661, RRID: AB_10557420 Vinculin Sigma CAT#V9131, RRID: AB_477629 APC Goat anti-Mouse IgG Invitrogen CAT#A-865, RRID: AB_141364 Chemical, peptides, and recombinant proteins 2-mercaptoethanol Invitrogen 31350010-50mM 4 Diamidino 2 Phenylindole Sigma 32670-25MG-F 4-phenylbutyric Acid Sigma P21005 ACK Lysing buffer Invitrogen A10492 Albumin Bovine Fraction Sigma A8806 Approved Fbs South American EuroClone SpA ECS0165L Arachidonic Acid- BSA conjugated Aurogene OOCD00263 Calcein AM Life technologies C3100MP Colegenase from C. histolyticum Sigma C2674-1G CTS OpTmizer T Cell medium Invitrogen A1048501 D-( )-glucose Solution Sigma G8769 Deoxyribonuclease I Sigma D5025-375KU Etomoxir Sigma E1905-5MG Ficoll Paque Plus Sigma GE17-1440-03 GolgiPlug BD Biosciences 555029 Hcs Lipidtox Red Neutral Lipid Stain Life technologies H34476 Hoechst No. 33342 Sigma B2261 human AB serum AnProtec ACSM0071 Ionomycin Sigma I0634 Linoleic Acid - BSA conjugated Sigma L9530 MitoTracker Green FM Invitrogen M7514 MitoTracker Orange CMTMRos Invitrogen M7510 Nocodazole Sigma M1404 (Continued on next page) e2 Cell Metabolism 35, 633–650.e1–e9, April 4, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oleic Acid - BSA conjugated Sigma O3008 OVA peptide (SIINFEKL) SB-Peptide N/A Paraformaldehyde Invitrogen 043368.9L Penicillin/Streptomicin 100X EuroClone SpA ECB3001D PMA Sigma P1585 Recombinant human Interleukin 15 Miltenyi 130-095-762 Recombinant human Interleukin 2 Novartis CLB-P-476-800-13980 IT Recombinant human Interleukin 7 Miltenyi 130-095-361 Rpmi 1640 With Stable L-glutamine EuroClone SpA ECM2001L Seahorse Xf Ph 7.4 Rpmi Agilent 103681-100 Sodium Pyruvate Solution 100 Nm EuroClone SpA LOBE13115E Thapsigargin Sigma T9033 Triton X100 Vwr International 1.08603.1000 U-13C18 Linoleic acid Cambridge Isotopes CLM-6855 Critical commercial assays Acetyl-Coenzyme A Assay Kit Sigma MAK039 Atp 1 Determination Kit Invitrogen A22066 BCA Protein Assay kit ThermoScientific 23227 CD8+ T cell isolation Kit Human Miltenyi 130-096-495 CD8+ T cell isolation Kit Mouse Miltenyi 130-104-075 Clarity Western ECL substrate Biorad 170-5061 Foxp3 Fix/perm Buffer Set BD Biosciences 560409 Lactate Assay Kit Sigma MAK064 LS columns Miltenyi 130-042-401 RetroNectin Takara-Bio T110A Rneasy Minielute Cleanup Kit Qiagen 74204 Seahorse Xf Cell Mito Stress Test Kit Agilent 103015-100 Seahorse Xf Glycolytic Rate Test Kit Agilent 103344-100 Seahorse Xfe96 Fluxpak Mini Agilent 102601-100 Ultracomp Ebeads Affymetrix Invitrogen 01-2222-42 Deposited Western Blot:Article Title: Linoleic acid potentiates CD8 + T cell metabolic fitness and antitumor immunity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-mouse TCF1 - APC BD Biosciences CAT#566693, RRID: AB_2869823 anti-mouse TCR Vb5.2, 5.2 - PE Biolegend CAT#139504, RRID: AB_10613279 anti-mouse TCR Vb5.2, 5.2 - FITC Biolegend CAT#139514, RRID: AB_2750133 anti-mouse TIM3 - BV786 BD Biosciences CAT#747621, RRID: AB_2744187 anti-mouse TNF - BV650 BD Biosciences CAT#563943, RRID: AB_2738498 anti-mouse/human TOX - APC Miltenyi Biotec CAT#130-118-335, RRID: AB_2751485 CFSE BD Biosciences CAT#565082, RRID: AB_2869649 CD3 Monoclonal Antibody (OKT3) Invitrogen CAT#16-0037-81, RRID: AB_468854 Cleaved Caspase-3 (Asp175) Cell signaling CAT#9661, RRID: AB_2069869 Far-Red Fluorescent Live-Cell Impermeant DNA Dye DRAQ7 BioStatus Limited DR71000 Fixable Viability Dye 640/662 Biotum 32007A Fixable viability Stain 510 BD Biosciences CAT#564406, RRID: AB_2869572 GRP78 Invitrogen CAT#PA1014A, RRID: AB_559362 Histone H3 Abcam CAT#ab1791, RRID: AB_302613 OXPHOS Abcam CAT#ab110413, RRID: AB_2629281 Phospho-s6 Ser240/244 Cell signaling CAT#5364, RRID: AB_10694233 Purified anti-human Cd28 BD Biosciences CAT#556620, RRID: AB_396492 Purified anti-human Cd3 BD Biosciences CAT#551916, RRID: AB_394293 Purified anti-mouse Cd28 BD Biosciences CAT#553294, RRID: AB_394763 Purified anti-mouse Cd3 BD Biosciences CAT#553057, RRID: AB_394590 Alexa488-affini Donkey Anti-rabbit IgG Invitrogen CAT#A-21206, RRID: AB_2535792 VDAC (D73D12) Cell signaling CAT#4661, RRID: AB_10557420 Vinculin Sigma CAT#V9131, RRID: AB_477629 APC Goat anti-Mouse IgG Invitrogen CAT#A-865, RRID: AB_141364 Chemical, peptides, and recombinant proteins 2-mercaptoethanol Invitrogen 31350010-50mM 4 Diamidino 2 Phenylindole Sigma 32670-25MG-F 4-phenylbutyric Acid Sigma P21005 ACK Lysing buffer Invitrogen A10492 Albumin Bovine Fraction Sigma A8806 Approved Fbs South American EuroClone SpA ECS0165L Arachidonic Acid- BSA conjugated Aurogene OOCD00263 Calcein AM Life technologies C3100MP Colegenase from C. histolyticum Sigma C2674-1G CTS OpTmizer T Cell medium Invitrogen A1048501 D-( )-glucose Solution Sigma G8769 Deoxyribonuclease I Sigma D5025-375KU Etomoxir Sigma E1905-5MG Ficoll Paque Plus Sigma GE17-1440-03 GolgiPlug BD Biosciences 555029 Hcs Lipidtox Red Neutral Lipid Stain Life technologies H34476 Hoechst No. 33342 Sigma B2261 human AB serum AnProtec ACSM0071 Ionomycin Sigma I0634 Linoleic Acid - BSA conjugated Sigma L9530 MitoTracker Green FM Invitrogen M7514 MitoTracker Orange CMTMRos Invitrogen M7510 Nocodazole Sigma M1404 (Continued on next page) e2 Cell Metabolism 35, 633–650.e1–e9, April 4, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oleic Acid - BSA conjugated Sigma O3008 OVA peptide (SIINFEKL) SB-Peptide N/A Paraformaldehyde Invitrogen 043368.9L Penicillin/Streptomicin 100X EuroClone SpA ECB3001D PMA Sigma P1585 Recombinant human Interleukin 15 Miltenyi 130-095-762 Recombinant human Interleukin 2 Novartis CLB-P-476-800-13980 IT Recombinant human Interleukin 7 Miltenyi 130-095-361 Rpmi 1640 With Stable L-glutamine EuroClone SpA ECM2001L Seahorse Xf Ph 7.4 Rpmi Agilent 103681-100 Sodium Pyruvate Solution 100 Nm EuroClone SpA LOBE13115E Thapsigargin Sigma T9033 Triton X100 Vwr International 1.08603.1000 U-13C18 Linoleic acid Cambridge Isotopes CLM-6855 Critical commercial assays Acetyl-Coenzyme A Assay Kit Sigma MAK039 Atp 1 Determination Kit Invitrogen A22066 BCA Protein Assay kit ThermoScientific 23227 CD8+ T cell isolation Kit Human Miltenyi 130-096-495 CD8+ T cell isolation Kit Mouse Miltenyi 130-104-075 Clarity Western ECL substrate Biorad 170-5061 Foxp3 Fix/perm Buffer Set BD Biosciences 560409 Lactate Assay Kit Sigma MAK064 LS columns Miltenyi 130-042-401 RetroNectin Takara-Bio T110A Rneasy Minielute Cleanup Kit Qiagen 74204 Seahorse Xf Cell Mito Stress Test Kit Agilent 103015-100 Seahorse Xf Glycolytic Rate Test Kit Agilent 103344-100 Seahorse Xfe96 Fluxpak Mini Agilent 102601-100 Ultracomp Ebeads Affymetrix Invitrogen 01-2222-42 Deposited Lactate Assay:Article Title: Linoleic acid potentiates CD8 + T cell metabolic fitness and antitumor immunity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-mouse TCF1 - APC BD Biosciences CAT#566693, RRID: AB_2869823 anti-mouse TCR Vb5.2, 5.2 - PE Biolegend CAT#139504, RRID: AB_10613279 anti-mouse TCR Vb5.2, 5.2 - FITC Biolegend CAT#139514, RRID: AB_2750133 anti-mouse TIM3 - BV786 BD Biosciences CAT#747621, RRID: AB_2744187 anti-mouse TNF - BV650 BD Biosciences CAT#563943, RRID: AB_2738498 anti-mouse/human TOX - APC Miltenyi Biotec CAT#130-118-335, RRID: AB_2751485 CFSE BD Biosciences CAT#565082, RRID: AB_2869649 CD3 Monoclonal Antibody (OKT3) Invitrogen CAT#16-0037-81, RRID: AB_468854 Cleaved Caspase-3 (Asp175) Cell signaling CAT#9661, RRID: AB_2069869 Far-Red Fluorescent Live-Cell Impermeant DNA Dye DRAQ7 BioStatus Limited DR71000 Fixable Viability Dye 640/662 Biotum 32007A Fixable viability Stain 510 BD Biosciences CAT#564406, RRID: AB_2869572 GRP78 Invitrogen CAT#PA1014A, RRID: AB_559362 Histone H3 Abcam CAT#ab1791, RRID: AB_302613 OXPHOS Abcam CAT#ab110413, RRID: AB_2629281 Phospho-s6 Ser240/244 Cell signaling CAT#5364, RRID: AB_10694233 Purified anti-human Cd28 BD Biosciences CAT#556620, RRID: AB_396492 Purified anti-human Cd3 BD Biosciences CAT#551916, RRID: AB_394293 Purified anti-mouse Cd28 BD Biosciences CAT#553294, RRID: AB_394763 Purified anti-mouse Cd3 BD Biosciences CAT#553057, RRID: AB_394590 Alexa488-affini Donkey Anti-rabbit IgG Invitrogen CAT#A-21206, RRID: AB_2535792 VDAC (D73D12) Cell signaling CAT#4661, RRID: AB_10557420 Vinculin Sigma CAT#V9131, RRID: AB_477629 APC Goat anti-Mouse IgG Invitrogen CAT#A-865, RRID: AB_141364 Chemical, peptides, and recombinant proteins 2-mercaptoethanol Invitrogen 31350010-50mM 4 Diamidino 2 Phenylindole Sigma 32670-25MG-F 4-phenylbutyric Acid Sigma P21005 ACK Lysing buffer Invitrogen A10492 Albumin Bovine Fraction Sigma A8806 Approved Fbs South American EuroClone SpA ECS0165L Arachidonic Acid- BSA conjugated Aurogene OOCD00263 Calcein AM Life technologies C3100MP Colegenase from C. histolyticum Sigma C2674-1G CTS OpTmizer T Cell medium Invitrogen A1048501 D-( )-glucose Solution Sigma G8769 Deoxyribonuclease I Sigma D5025-375KU Etomoxir Sigma E1905-5MG Ficoll Paque Plus Sigma GE17-1440-03 GolgiPlug BD Biosciences 555029 Hcs Lipidtox Red Neutral Lipid Stain Life technologies H34476 Hoechst No. 33342 Sigma B2261 human AB serum AnProtec ACSM0071 Ionomycin Sigma I0634 Linoleic Acid - BSA conjugated Sigma L9530 MitoTracker Green FM Invitrogen M7514 MitoTracker Orange CMTMRos Invitrogen M7510 Nocodazole Sigma M1404 (Continued on next page) e2 Cell Metabolism 35, 633–650.e1–e9, April 4, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oleic Acid - BSA conjugated Sigma O3008 OVA peptide (SIINFEKL) SB-Peptide N/A Paraformaldehyde Invitrogen 043368.9L Penicillin/Streptomicin 100X EuroClone SpA ECB3001D PMA Sigma P1585 Recombinant human Interleukin 15 Miltenyi 130-095-762 Recombinant human Interleukin 2 Novartis CLB-P-476-800-13980 IT Recombinant human Interleukin 7 Miltenyi 130-095-361 Rpmi 1640 With Stable L-glutamine EuroClone SpA ECM2001L Seahorse Xf Ph 7.4 Rpmi Agilent 103681-100 Sodium Pyruvate Solution 100 Nm EuroClone SpA LOBE13115E Thapsigargin Sigma T9033 Triton X100 Vwr International 1.08603.1000 U-13C18 Linoleic acid Cambridge Isotopes CLM-6855 Critical commercial assays Acetyl-Coenzyme A Assay Kit Sigma MAK039 Atp 1 Determination Kit Invitrogen A22066 BCA Protein Assay kit ThermoScientific 23227 CD8+ T cell isolation Kit Human Miltenyi 130-096-495 CD8+ T cell isolation Kit Mouse Miltenyi 130-104-075 Clarity Western ECL substrate Biorad 170-5061 Foxp3 Fix/perm Buffer Set BD Biosciences 560409 Lactate Assay Kit Sigma MAK064 LS columns Miltenyi 130-042-401 RetroNectin Takara-Bio T110A Rneasy Minielute Cleanup Kit Qiagen 74204 Seahorse Xf Cell Mito Stress Test Kit Agilent 103015-100 Seahorse Xf Glycolytic Rate Test Kit Agilent 103344-100 Seahorse Xfe96 Fluxpak Mini Agilent 102601-100 Ultracomp Ebeads Affymetrix Invitrogen 01-2222-42 Deposited RNA sequencing:Article Title: Linoleic acid potentiates CD8 + T cell metabolic fitness and antitumor immunity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-mouse TCF1 - APC BD Biosciences CAT#566693, RRID: AB_2869823 anti-mouse TCR Vb5.2, 5.2 - PE Biolegend CAT#139504, RRID: AB_10613279 anti-mouse TCR Vb5.2, 5.2 - FITC Biolegend CAT#139514, RRID: AB_2750133 anti-mouse TIM3 - BV786 BD Biosciences CAT#747621, RRID: AB_2744187 anti-mouse TNF - BV650 BD Biosciences CAT#563943, RRID: AB_2738498 anti-mouse/human TOX - APC Miltenyi Biotec CAT#130-118-335, RRID: AB_2751485 CFSE BD Biosciences CAT#565082, RRID: AB_2869649 CD3 Monoclonal Antibody (OKT3) Invitrogen CAT#16-0037-81, RRID: AB_468854 Cleaved Caspase-3 (Asp175) Cell signaling CAT#9661, RRID: AB_2069869 Far-Red Fluorescent Live-Cell Impermeant DNA Dye DRAQ7 BioStatus Limited DR71000 Fixable Viability Dye 640/662 Biotum 32007A Fixable viability Stain 510 BD Biosciences CAT#564406, RRID: AB_2869572 GRP78 Invitrogen CAT#PA1014A, RRID: AB_559362 Histone H3 Abcam CAT#ab1791, RRID: AB_302613 OXPHOS Abcam CAT#ab110413, RRID: AB_2629281 Phospho-s6 Ser240/244 Cell signaling CAT#5364, RRID: AB_10694233 Purified anti-human Cd28 BD Biosciences CAT#556620, RRID: AB_396492 Purified anti-human Cd3 BD Biosciences CAT#551916, RRID: AB_394293 Purified anti-mouse Cd28 BD Biosciences CAT#553294, RRID: AB_394763 Purified anti-mouse Cd3 BD Biosciences CAT#553057, RRID: AB_394590 Alexa488-affini Donkey Anti-rabbit IgG Invitrogen CAT#A-21206, RRID: AB_2535792 VDAC (D73D12) Cell signaling CAT#4661, RRID: AB_10557420 Vinculin Sigma CAT#V9131, RRID: AB_477629 APC Goat anti-Mouse IgG Invitrogen CAT#A-865, RRID: AB_141364 Chemical, peptides, and recombinant proteins 2-mercaptoethanol Invitrogen 31350010-50mM 4 Diamidino 2 Phenylindole Sigma 32670-25MG-F 4-phenylbutyric Acid Sigma P21005 ACK Lysing buffer Invitrogen A10492 Albumin Bovine Fraction Sigma A8806 Approved Fbs South American EuroClone SpA ECS0165L Arachidonic Acid- BSA conjugated Aurogene OOCD00263 Calcein AM Life technologies C3100MP Colegenase from C. histolyticum Sigma C2674-1G CTS OpTmizer T Cell medium Invitrogen A1048501 D-( )-glucose Solution Sigma G8769 Deoxyribonuclease I Sigma D5025-375KU Etomoxir Sigma E1905-5MG Ficoll Paque Plus Sigma GE17-1440-03 GolgiPlug BD Biosciences 555029 Hcs Lipidtox Red Neutral Lipid Stain Life technologies H34476 Hoechst No. 33342 Sigma B2261 human AB serum AnProtec ACSM0071 Ionomycin Sigma I0634 Linoleic Acid - BSA conjugated Sigma L9530 MitoTracker Green FM Invitrogen M7514 MitoTracker Orange CMTMRos Invitrogen M7510 Nocodazole Sigma M1404 (Continued on next page) e2 Cell Metabolism 35, 633–650.e1–e9, April 4, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oleic Acid - BSA conjugated Sigma O3008 OVA peptide (SIINFEKL) SB-Peptide N/A Paraformaldehyde Invitrogen 043368.9L Penicillin/Streptomicin 100X EuroClone SpA ECB3001D PMA Sigma P1585 Recombinant human Interleukin 15 Miltenyi 130-095-762 Recombinant human Interleukin 2 Novartis CLB-P-476-800-13980 IT Recombinant human Interleukin 7 Miltenyi 130-095-361 Rpmi 1640 With Stable L-glutamine EuroClone SpA ECM2001L Seahorse Xf Ph 7.4 Rpmi Agilent 103681-100 Sodium Pyruvate Solution 100 Nm EuroClone SpA LOBE13115E Thapsigargin Sigma T9033 Triton X100 Vwr International 1.08603.1000 U-13C18 Linoleic acid Cambridge Isotopes CLM-6855 Critical commercial assays Acetyl-Coenzyme A Assay Kit Sigma MAK039 Atp 1 Determination Kit Invitrogen A22066 BCA Protein Assay kit ThermoScientific 23227 CD8+ T cell isolation Kit Human Miltenyi 130-096-495 CD8+ T cell isolation Kit Mouse Miltenyi 130-104-075 Clarity Western ECL substrate Biorad 170-5061 Foxp3 Fix/perm Buffer Set BD Biosciences 560409 Lactate Assay Kit Sigma MAK064 LS columns Miltenyi 130-042-401 RetroNectin Takara-Bio T110A Rneasy Minielute Cleanup Kit Qiagen 74204 Seahorse Xf Cell Mito Stress Test Kit Agilent 103015-100 Seahorse Xf Glycolytic Rate Test Kit Agilent 103344-100 Seahorse Xfe96 Fluxpak Mini Agilent 102601-100 Ultracomp Ebeads Affymetrix Invitrogen 01-2222-42 Deposited Software:Article Title: Linoleic acid potentiates CD8 + T cell metabolic fitness and antitumor immunity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER anti-mouse TCF1 - APC BD Biosciences CAT#566693, RRID: AB_2869823 anti-mouse TCR Vb5.2, 5.2 - PE Biolegend CAT#139504, RRID: AB_10613279 anti-mouse TCR Vb5.2, 5.2 - FITC Biolegend CAT#139514, RRID: AB_2750133 anti-mouse TIM3 - BV786 BD Biosciences CAT#747621, RRID: AB_2744187 anti-mouse TNF - BV650 BD Biosciences CAT#563943, RRID: AB_2738498 anti-mouse/human TOX - APC Miltenyi Biotec CAT#130-118-335, RRID: AB_2751485 CFSE BD Biosciences CAT#565082, RRID: AB_2869649 CD3 Monoclonal Antibody (OKT3) Invitrogen CAT#16-0037-81, RRID: AB_468854 Cleaved Caspase-3 (Asp175) Cell signaling CAT#9661, RRID: AB_2069869 Far-Red Fluorescent Live-Cell Impermeant DNA Dye DRAQ7 BioStatus Limited DR71000 Fixable Viability Dye 640/662 Biotum 32007A Fixable viability Stain 510 BD Biosciences CAT#564406, RRID: AB_2869572 GRP78 Invitrogen CAT#PA1014A, RRID: AB_559362 Histone H3 Abcam CAT#ab1791, RRID: AB_302613 OXPHOS Abcam CAT#ab110413, RRID: AB_2629281 Phospho-s6 Ser240/244 Cell signaling CAT#5364, RRID: AB_10694233 Purified anti-human Cd28 BD Biosciences CAT#556620, RRID: AB_396492 Purified anti-human Cd3 BD Biosciences CAT#551916, RRID: AB_394293 Purified anti-mouse Cd28 BD Biosciences CAT#553294, RRID: AB_394763 Purified anti-mouse Cd3 BD Biosciences CAT#553057, RRID: AB_394590 Alexa488-affini Donkey Anti-rabbit IgG Invitrogen CAT#A-21206, RRID: AB_2535792 VDAC (D73D12) Cell signaling CAT#4661, RRID: AB_10557420 Vinculin Sigma CAT#V9131, RRID: AB_477629 APC Goat anti-Mouse IgG Invitrogen CAT#A-865, RRID: AB_141364 Chemical, peptides, and recombinant proteins 2-mercaptoethanol Invitrogen 31350010-50mM 4 Diamidino 2 Phenylindole Sigma 32670-25MG-F 4-phenylbutyric Acid Sigma P21005 ACK Lysing buffer Invitrogen A10492 Albumin Bovine Fraction Sigma A8806 Approved Fbs South American EuroClone SpA ECS0165L Arachidonic Acid- BSA conjugated Aurogene OOCD00263 Calcein AM Life technologies C3100MP Colegenase from C. histolyticum Sigma C2674-1G CTS OpTmizer T Cell medium Invitrogen A1048501 D-( )-glucose Solution Sigma G8769 Deoxyribonuclease I Sigma D5025-375KU Etomoxir Sigma E1905-5MG Ficoll Paque Plus Sigma GE17-1440-03 GolgiPlug BD Biosciences 555029 Hcs Lipidtox Red Neutral Lipid Stain Life technologies H34476 Hoechst No. 33342 Sigma B2261 human AB serum AnProtec ACSM0071 Ionomycin Sigma I0634 Linoleic Acid - BSA conjugated Sigma L9530 MitoTracker Green FM Invitrogen M7514 MitoTracker Orange CMTMRos Invitrogen M7510 Nocodazole Sigma M1404 (Continued on next page) e2 Cell Metabolism 35, 633–650.e1–e9, April 4, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oleic Acid - BSA conjugated Sigma O3008 OVA peptide (SIINFEKL) SB-Peptide N/A Paraformaldehyde Invitrogen 043368.9L Penicillin/Streptomicin 100X EuroClone SpA ECB3001D PMA Sigma P1585 Recombinant human Interleukin 15 Miltenyi 130-095-762 Recombinant human Interleukin 2 Novartis CLB-P-476-800-13980 IT Recombinant human Interleukin 7 Miltenyi 130-095-361 Rpmi 1640 With Stable L-glutamine EuroClone SpA ECM2001L Seahorse Xf Ph 7.4 Rpmi Agilent 103681-100 Sodium Pyruvate Solution 100 Nm EuroClone SpA LOBE13115E Thapsigargin Sigma T9033 Triton X100 Vwr International 1.08603.1000 U-13C18 Linoleic acid Cambridge Isotopes CLM-6855 Critical commercial assays Acetyl-Coenzyme A Assay Kit Sigma MAK039 Atp 1 Determination Kit Invitrogen A22066 BCA Protein Assay kit ThermoScientific 23227 CD8+ T cell isolation Kit Human Miltenyi 130-096-495 CD8+ T cell isolation Kit Mouse Miltenyi 130-104-075 Clarity Western ECL substrate Biorad 170-5061 Foxp3 Fix/perm Buffer Set BD Biosciences 560409 Lactate Assay Kit Sigma MAK064 LS columns Miltenyi 130-042-401 RetroNectin Takara-Bio T110A Rneasy Minielute Cleanup Kit Qiagen 74204 Seahorse Xf Cell Mito Stress Test Kit Agilent 103015-100 Seahorse Xf Glycolytic Rate Test Kit Agilent 103344-100 Seahorse Xfe96 Fluxpak Mini Agilent 102601-100 Ultracomp Ebeads Affymetrix Invitrogen 01-2222-42 Deposited |
