Review

tetramethylbenzidine substrate (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher tetramethylbenzidine substrate
Tetramethylbenzidine Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/3%2C3'%2C5%2C5'-Tetramethylbenzidine+soln%2E%2C+Ready-to-Use%2C+high+sensitivity/pmc04894690-207-5-7
Average 95 stars, based on 1 article reviews

tetramethylbenzidine substrate - by Bioz Stars, 2026-10

95/100 stars

Images

other:

Article Title: Human anti-alpha-hemolysin scFvs mitigate toxin-mediated damage in a mouse model of MRSA pneumonia.
Article Snippet: The color reaction was developed using a 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution (Thermo Scientific, MA, USA), followed by the addition of 1 M H2SO4 (Merck) (Piri-Gavgani et al. 2022).

Article Title: Analysis of West Nile disease convalescents identifies human monoclonal antibodies protective against West Nile and related orthoflaviviruses.
Article Snippet: After the final washes, TMB (3,3 ′ ,5,5 ′ -Tetramethylbenzidine) substrate (Thermo Fisher Scientific, 34021) was added to develop the plate, 1M sulfuric acid was added to stop the reaction, and the plates read at 450nm at the luminometer (BioTek, EPOCH2 microplate reader).

Article Title: BCMA/TACI antigen-binding molecules
Article Snippet: Briefly, wells of 96-well plates (Nunc, Denmark) are coated with 6 μg/ml of untagged human CD47 protein in PBS, for 16 hrs at 4° C. After blocking for 1 h 30 min with 1% BSA in 1×PBS at room temperature, his-tagged human BCMA (6 μg/ml) is added to the wells, along with 10 μg/ml of the bispecific anti-BCMA, anti-CD47 antibodies, and the plates are incubated at room temperature for 1 h. Plates are then washed three times with 1×PBS containing 0.05% Tween 20, and incubated with a HRP-conjugated anti-His antibody (Life Technologies, Inc., USA) at a 1:5000 dilution, for 1 h at room temperature.

Article Title: Widespread circulation of Alongshan virus in Austria and serological evidence for infection in humans: a nationwide molecular and serological observational study.
Article Snippet: Tetramethylbenzidine substrate (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific) was added, and absorbance was measured at 450 or 630 nm after quenching the reaction with 1 M sulphuric acid.

Incubation:

Article Title: Proinsulin peptides for Type 1 Diabetes
Article Snippet: Autoantibody ELISA Proinsulin-2 peptides or recombinant human GAD65 were coated onto Maxisorp plates (Nunc, Roskilde, Denmark) in ELISA coating buffer (eBioscience). .. Diluted sera (in PBS 5% BSA, Sigma) or controls (mouse IgG2a anti-insulin clone ICBTACLS and isotype from ThermoFisher, mouse IgG1 anti-GAD65 clone N-GAD65 and isotype from Biolegend) were incubated for 2 hours at room temperature prior to detection with biotin-anti-mouse IgG, Streptavidin-HRP and high sensitivity TMB solution (all from eBioscience) and read at 450 nm. ..

Article Title: Co-delivered PD-L1 rescues the protective efficacy mediated by an AAV-expressed HIV-1 bNAb
Article Snippet: For ADA ELISAs, a 1:8000 dilution of an HRP-conjugated anti-human lambda light chain antibody (Millipore Sigma) was used to quantify 3BNC117 binding antibodies. .. The plates were then incubated at 37°C for 1 h, after which they were washed ten times with PBS-T. 3,3’,5,5’-Tetramethylbenzidine (TMB) Substrate Solution (Thermo Fisher Scientific) was added to the plates. ..

Enzyme-linked Immunosorbent Assay:

Article Title: 3D printing monolithic, multifunctional polymer acoustofluidic devices with tunable mixing and particle focusing
Article Snippet: .. Subsequently, 150 μL of 3,3′,5,5′-tetramethylbenzidine (TMB) (1-StepTM TMB ELISA Substrate Solution, Thermo ScientificTM) was added to the magnetically separated complex, resulting in the development of a blue color. .. Afterward, 200 μL of 2.5 M sulfuric acid (ACS reagent, 95.0–98.0%, Sigma-Aldrich) was added as a stop solution, causing the solution to turn yellow.

Binding Assay:

Article Title: Impact of Stiffness and Cell-Binding Motif Availability on the Cell-Specific Response to Collagen-Based Macromolecular Materials.
Article Snippet: .. The wells were washed with binding buffer (3 × 600 μL) and then a 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (300 μL)(Thermo Scientific) was added to each well and the reaction was stopped, after color had developed, by the addition of 2.5 M H2SO4 (300 μL) to each well. .. Solution from each well (100 μL) was pipetted into standard 96-well plates and the absorbance measured at 450 nm (A450) in a Fluostar Optima plate reader (BMG Labtech).