tetramethylbenzidine Search Results


3 3 5 5 tetramethylbenzidine  (Rockland Immunochemicals)
93
Rockland Immunochemicals 3 3 5 5 tetramethylbenzidine
3 3 5 5 Tetramethylbenzidine, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/pmc10697721-196-23-25?v=Rockland+Immunochemicals
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5 5  (Thermo Fisher)
97
Thermo Fisher 5 5
5 5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/pmc13006313-172-6-9?v=Thermo+Fisher
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5 5 - by Bioz Stars, 2026-07
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3 30 5 50 tetramethylbenzidine  (Thermo Fisher)
96
Thermo Fisher 3 30 5 50 tetramethylbenzidine
3 30 5 50 Tetramethylbenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/10__1158_slash_1535___7163__mct___19___0515-68-9-11?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
3 30 5 50 tetramethylbenzidine - by Bioz Stars, 2026-07
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3 30 5 50 tetramethylbenzidine tmb  (Thermo Fisher)
94
Thermo Fisher 3 30 5 50 tetramethylbenzidine tmb
3 30 5 50 Tetramethylbenzidine Tmb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/bio_rxiv__64898__2026__05__04__722690-183-7-9?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
3 30 5 50 tetramethylbenzidine tmb - by Bioz Stars, 2026-07
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tetramethylbenzidine  (Thermo Fisher)
95
Thermo Fisher tetramethylbenzidine
Tetramethylbenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/10__3390_slash_antib13040104-81-50-51?v=Thermo+Fisher
Average 95 stars, based on 1 article reviews
tetramethylbenzidine - by Bioz Stars, 2026-07
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chromogenic substrate tetramethylbenzidine tmb  (Thermo Fisher)
94
Thermo Fisher chromogenic substrate tetramethylbenzidine tmb
( A ) Sequence alignment of the 18 AIM2 nanobody candidates. Complementarity-determining regions (CDRs) are indicated. ( B , C ) Binding of the indicated recombinant HA-tagged nanobody candidates (1 µM VHH AIM2-(1–6) -HA-His) and (0.1 µM VHH AIM2-(7-18) -HA-His) to immobilized MBP-AIM2 PYD or control protein MBP was quantified by ELISA with anti-HA HRP antibodies and the <t>chromogenic</t> substrate <t>TMB.</t> Nanobodies identified by phage display with full-length eukaryotically expressed AIM2-SH ( B ) or bacterially expressed MBP-AIM2 PYD ( C ) were analyzed, and absorption at 450 nm (A450) is displayed. ( D , E ) Binding of indicated nanobodies in the cytosol was quantified by LUMIER assay. HEK293T cells were co-transfected with expression vectors for the specified HA-tagged nanobodies and the indicated proteins fused to Renilla luciferase (mAIM2 indicates murine AIM2). 24 h post transfection, cells were lysed and VHH-HA was immunoprecipitated with immobilized anti-HA antibody. Coelenterazine-h was added and luminescence of co-purified Renilla luciferase was measured. Binding to full-length mouse/human AIM2 ( D ), or to different AIM2 domains ( E ) was analyzed. ( F ) Competition ELISA: MBP-AIM2 PYD was immobilized to ELISA plates, and binding of 1 µM of the indicated HA-His tagged nanobodies in the presence of an increasing concentration of VHH AIM2-1 -LPETG-His, VHH AIM2-2 -LPETG-His or control nanobody VHH NP-1 -LPETG-His was quantified by ELISA with anti-HA HRP antibodies and TMB as in ( A ). Values were normalized to binding in the absence of VHH-LPETG-His. Data represent average values from N = 3 independent experiments ± SEM. Individual data points are shown for ( B – E ). .
Chromogenic Substrate Tetramethylbenzidine Tmb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/pmc12953764-548-12-16?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
chromogenic substrate tetramethylbenzidine tmb - by Bioz Stars, 2026-07
94/100 stars
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tetramethylbenzidine substrate  (Thermo Fisher)
95
Thermo Fisher tetramethylbenzidine substrate
( A ) Sequence alignment of the 18 AIM2 nanobody candidates. Complementarity-determining regions (CDRs) are indicated. ( B , C ) Binding of the indicated recombinant HA-tagged nanobody candidates (1 µM VHH AIM2-(1–6) -HA-His) and (0.1 µM VHH AIM2-(7-18) -HA-His) to immobilized MBP-AIM2 PYD or control protein MBP was quantified by ELISA with anti-HA HRP antibodies and the <t>chromogenic</t> substrate <t>TMB.</t> Nanobodies identified by phage display with full-length eukaryotically expressed AIM2-SH ( B ) or bacterially expressed MBP-AIM2 PYD ( C ) were analyzed, and absorption at 450 nm (A450) is displayed. ( D , E ) Binding of indicated nanobodies in the cytosol was quantified by LUMIER assay. HEK293T cells were co-transfected with expression vectors for the specified HA-tagged nanobodies and the indicated proteins fused to Renilla luciferase (mAIM2 indicates murine AIM2). 24 h post transfection, cells were lysed and VHH-HA was immunoprecipitated with immobilized anti-HA antibody. Coelenterazine-h was added and luminescence of co-purified Renilla luciferase was measured. Binding to full-length mouse/human AIM2 ( D ), or to different AIM2 domains ( E ) was analyzed. ( F ) Competition ELISA: MBP-AIM2 PYD was immobilized to ELISA plates, and binding of 1 µM of the indicated HA-His tagged nanobodies in the presence of an increasing concentration of VHH AIM2-1 -LPETG-His, VHH AIM2-2 -LPETG-His or control nanobody VHH NP-1 -LPETG-His was quantified by ELISA with anti-HA HRP antibodies and TMB as in ( A ). Values were normalized to binding in the absence of VHH-LPETG-His. Data represent average values from N = 3 independent experiments ± SEM. Individual data points are shown for ( B – E ). .
Tetramethylbenzidine Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/pmc04894690-207-5-7?v=Thermo+Fisher
Average 95 stars, based on 1 article reviews
tetramethylbenzidine substrate - by Bioz Stars, 2026-07
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3 3 5 5 tetramethylbenzidine  (Thermo Fisher)
94
Thermo Fisher 3 3 5 5 tetramethylbenzidine
( A ) Sequence alignment of the 18 AIM2 nanobody candidates. Complementarity-determining regions (CDRs) are indicated. ( B , C ) Binding of the indicated recombinant HA-tagged nanobody candidates (1 µM VHH AIM2-(1–6) -HA-His) and (0.1 µM VHH AIM2-(7-18) -HA-His) to immobilized MBP-AIM2 PYD or control protein MBP was quantified by ELISA with anti-HA HRP antibodies and the <t>chromogenic</t> substrate <t>TMB.</t> Nanobodies identified by phage display with full-length eukaryotically expressed AIM2-SH ( B ) or bacterially expressed MBP-AIM2 PYD ( C ) were analyzed, and absorption at 450 nm (A450) is displayed. ( D , E ) Binding of indicated nanobodies in the cytosol was quantified by LUMIER assay. HEK293T cells were co-transfected with expression vectors for the specified HA-tagged nanobodies and the indicated proteins fused to Renilla luciferase (mAIM2 indicates murine AIM2). 24 h post transfection, cells were lysed and VHH-HA was immunoprecipitated with immobilized anti-HA antibody. Coelenterazine-h was added and luminescence of co-purified Renilla luciferase was measured. Binding to full-length mouse/human AIM2 ( D ), or to different AIM2 domains ( E ) was analyzed. ( F ) Competition ELISA: MBP-AIM2 PYD was immobilized to ELISA plates, and binding of 1 µM of the indicated HA-His tagged nanobodies in the presence of an increasing concentration of VHH AIM2-1 -LPETG-His, VHH AIM2-2 -LPETG-His or control nanobody VHH NP-1 -LPETG-His was quantified by ELISA with anti-HA HRP antibodies and TMB as in ( A ). Values were normalized to binding in the absence of VHH-LPETG-His. Data represent average values from N = 3 independent experiments ± SEM. Individual data points are shown for ( B – E ). .
3 3 5 5 Tetramethylbenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/pm37845505-41-97-100?v=Thermo+Fisher
Average 94 stars, based on 1 article reviews
3 3 5 5 tetramethylbenzidine - by Bioz Stars, 2026-07
94/100 stars
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tmbe  (Rockland Immunochemicals)
94
Rockland Immunochemicals tmbe
( A ) Sequence alignment of the 18 AIM2 nanobody candidates. Complementarity-determining regions (CDRs) are indicated. ( B , C ) Binding of the indicated recombinant HA-tagged nanobody candidates (1 µM VHH AIM2-(1–6) -HA-His) and (0.1 µM VHH AIM2-(7-18) -HA-His) to immobilized MBP-AIM2 PYD or control protein MBP was quantified by ELISA with anti-HA HRP antibodies and the <t>chromogenic</t> substrate <t>TMB.</t> Nanobodies identified by phage display with full-length eukaryotically expressed AIM2-SH ( B ) or bacterially expressed MBP-AIM2 PYD ( C ) were analyzed, and absorption at 450 nm (A450) is displayed. ( D , E ) Binding of indicated nanobodies in the cytosol was quantified by LUMIER assay. HEK293T cells were co-transfected with expression vectors for the specified HA-tagged nanobodies and the indicated proteins fused to Renilla luciferase (mAIM2 indicates murine AIM2). 24 h post transfection, cells were lysed and VHH-HA was immunoprecipitated with immobilized anti-HA antibody. Coelenterazine-h was added and luminescence of co-purified Renilla luciferase was measured. Binding to full-length mouse/human AIM2 ( D ), or to different AIM2 domains ( E ) was analyzed. ( F ) Competition ELISA: MBP-AIM2 PYD was immobilized to ELISA plates, and binding of 1 µM of the indicated HA-His tagged nanobodies in the presence of an increasing concentration of VHH AIM2-1 -LPETG-His, VHH AIM2-2 -LPETG-His or control nanobody VHH NP-1 -LPETG-His was quantified by ELISA with anti-HA HRP antibodies and TMB as in ( A ). Values were normalized to binding in the absence of VHH-LPETG-His. Data represent average values from N = 3 independent experiments ± SEM. Individual data points are shown for ( B – E ). .
Tmbe, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tetramethylbenzidine/pm41406942-309-18-19?v=Rockland+Immunochemicals
Average 94 stars, based on 1 article reviews
tmbe - by Bioz Stars, 2026-07
94/100 stars
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Image Search Results


( A ) Sequence alignment of the 18 AIM2 nanobody candidates. Complementarity-determining regions (CDRs) are indicated. ( B , C ) Binding of the indicated recombinant HA-tagged nanobody candidates (1 µM VHH AIM2-(1–6) -HA-His) and (0.1 µM VHH AIM2-(7-18) -HA-His) to immobilized MBP-AIM2 PYD or control protein MBP was quantified by ELISA with anti-HA HRP antibodies and the chromogenic substrate TMB. Nanobodies identified by phage display with full-length eukaryotically expressed AIM2-SH ( B ) or bacterially expressed MBP-AIM2 PYD ( C ) were analyzed, and absorption at 450 nm (A450) is displayed. ( D , E ) Binding of indicated nanobodies in the cytosol was quantified by LUMIER assay. HEK293T cells were co-transfected with expression vectors for the specified HA-tagged nanobodies and the indicated proteins fused to Renilla luciferase (mAIM2 indicates murine AIM2). 24 h post transfection, cells were lysed and VHH-HA was immunoprecipitated with immobilized anti-HA antibody. Coelenterazine-h was added and luminescence of co-purified Renilla luciferase was measured. Binding to full-length mouse/human AIM2 ( D ), or to different AIM2 domains ( E ) was analyzed. ( F ) Competition ELISA: MBP-AIM2 PYD was immobilized to ELISA plates, and binding of 1 µM of the indicated HA-His tagged nanobodies in the presence of an increasing concentration of VHH AIM2-1 -LPETG-His, VHH AIM2-2 -LPETG-His or control nanobody VHH NP-1 -LPETG-His was quantified by ELISA with anti-HA HRP antibodies and TMB as in ( A ). Values were normalized to binding in the absence of VHH-LPETG-His. Data represent average values from N = 3 independent experiments ± SEM. Individual data points are shown for ( B – E ). .

Journal: The EMBO Journal

Article Title: Poxvirus dsDNA genomes differentially activate AIM2 or NLRP3 inflammasomes in human primary cells

doi: 10.1038/s44318-025-00690-z

Figure Lengend Snippet: ( A ) Sequence alignment of the 18 AIM2 nanobody candidates. Complementarity-determining regions (CDRs) are indicated. ( B , C ) Binding of the indicated recombinant HA-tagged nanobody candidates (1 µM VHH AIM2-(1–6) -HA-His) and (0.1 µM VHH AIM2-(7-18) -HA-His) to immobilized MBP-AIM2 PYD or control protein MBP was quantified by ELISA with anti-HA HRP antibodies and the chromogenic substrate TMB. Nanobodies identified by phage display with full-length eukaryotically expressed AIM2-SH ( B ) or bacterially expressed MBP-AIM2 PYD ( C ) were analyzed, and absorption at 450 nm (A450) is displayed. ( D , E ) Binding of indicated nanobodies in the cytosol was quantified by LUMIER assay. HEK293T cells were co-transfected with expression vectors for the specified HA-tagged nanobodies and the indicated proteins fused to Renilla luciferase (mAIM2 indicates murine AIM2). 24 h post transfection, cells were lysed and VHH-HA was immunoprecipitated with immobilized anti-HA antibody. Coelenterazine-h was added and luminescence of co-purified Renilla luciferase was measured. Binding to full-length mouse/human AIM2 ( D ), or to different AIM2 domains ( E ) was analyzed. ( F ) Competition ELISA: MBP-AIM2 PYD was immobilized to ELISA plates, and binding of 1 µM of the indicated HA-His tagged nanobodies in the presence of an increasing concentration of VHH AIM2-1 -LPETG-His, VHH AIM2-2 -LPETG-His or control nanobody VHH NP-1 -LPETG-His was quantified by ELISA with anti-HA HRP antibodies and TMB as in ( A ). Values were normalized to binding in the absence of VHH-LPETG-His. Data represent average values from N = 3 independent experiments ± SEM. Individual data points are shown for ( B – E ). .

Article Snippet: VHH binding was detected with HRP-coupled rabbit anti-E-Tag antibodies (1:10,000), and the chromogenic substrate tetramethylbenzidine (TMB) (Life Technologies).

Techniques: Sequencing, Binding Assay, Recombinant, Control, Enzyme-linked Immunosorbent Assay, Transfection, Expressing, Luciferase, Immunoprecipitation, Purification, Concentration Assay