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superscript preamplification system kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript preamplification system kit
    Superscript Preamplification System Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+preamplification+system+kit/pm38453025-107-5-9
    Average 86 stars, based on 1 article reviews
    superscript preamplification system kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: Method of stimulating TLR9-activated immune response
    Article Snippet: First strand cDNA libraries were then synthesized from the collected total RNA samples using a SuperScriptTM preamplification kit (Invitrogen), and PCR amplifications were performed using an Expand HI Fid PCR kit (Roche).

    Synthesized:

    Article Title: Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening
    Article Snippet: The mRNA was enriched by using oligo (dT) magnetic beads (Illumina, San Diego, CA, USA) and cut into short fragments (about 200 bp). .. The first-strand cDNA was synthesized using a Superscript Preamplification System Kit (Gibco-BRL, Grand Island, NY, USA) as described in the manufacturer's instructions. ..

    Article Title: Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents, β-ionone and linalool
    Article Snippet: Total RNA was extracted from flower petals using a MiniBEST universal RNA extraction kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. RNA quality and integrity were assessed using formamide denaturing gel electrophoresis, and RNA quantity was measured using spectrophotometry . cDNA was then synthesized using a Superscript preamplification kit (Invitrogen, California, USA) according to the manufacturer’s instructions. .. Forward (ERF61f1) and reverse (ERF61r1) primers were designed and used to amplify the full-length ERF61 cDNA sequence under the following PCR amplification conditions: 94 °C for 4 min followed by 33 cycles of 94 °C for 30 s, 55 °C for 45 s, and 72 °C for 60 s. The PCR product was cloned into the pMD19-T vector (TaKaRa) and sequenced.

    Article Title: NAA10 Hypomethylation is associated with particulate matter exposure and worse prognosis for patients with non-small cell lung cancer
    Article Snippet: Total RNA was extracted from cultured cells and tissue specimens, using TRIzol reagent (Invitrogen, Waltham, MA, USA). .. After removing residual DNA, first-strand cDNA was synthesized from total RNA, using SuperScript preamplification kit (Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: Autocrine Prostaglandin E 2 Signaling Promotes Tumor Cell Survival and Proliferation in Childhood Neuroblastoma
    Article Snippet: The human myelocytic cell line, U-937 (ATCC, Boras, Sweden), was grown in PRMI using the same supplements as mentioned above. .. RNA was extracted from cells using TRIZOL reagent (Life Technologies Inc.,Carlsbad, CA, USA) according to the manufacturer's protocol, and cDNA was synthesized from 2.0 μg of RNA using a SuperScript Preamplification Kit (Life Technologies Inc.). .. The PCR was performed in 50 μL of reaction mixture containing 4 μl cDNA for EP1-4, and 2 μl for β-Actin (for SK-N-SH 4 μL was used), 1 unit of DyNAzymeII Recombinant DNA (FinnzymesOy, Espoo, Finland) and 1 μM of each primer.

    Reverse Transcription:

    Article Title: A novel RON splice variant lacking exon 2 activates the PI3K/AKT pathway via PTEN phosphorylation in colorectal carcinoma cells
    Article Snippet: Total RNAs were extracted three times from colorectal cancer cell lines and specimens using TRIzol (Life Technologies). .. Reverse transcription was carried out using 2 ug of total RNAs with a Superscript Preamplification Kit (Life Technologies). ..

    Amplification:

    Article Title: Role of microbiota-derived corisin in coagulation activation during SARS-CoV-2 infection.
    Article Snippet: Total RNA was extracted from HUVEC lysates using Trizol Reagent (Invitrogen). .. The oligo-dT primers and the Superscript Preamplification System Kit (Invitrogen) were used to reverse-transcribe RNA, and then DNA was amplified by PCR. ..

    Polymerase Chain Reaction:

    Article Title: Role of microbiota-derived corisin in coagulation activation during SARS-CoV-2 infection.
    Article Snippet: Total RNA was extracted from HUVEC lysates using Trizol Reagent (Invitrogen). .. The oligo-dT primers and the Superscript Preamplification System Kit (Invitrogen) were used to reverse-transcribe RNA, and then DNA was amplified by PCR. ..

    Nucleic Acid Electrophoresis:

    Article Title: Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents, β-ionone and linalool
    Article Snippet: Total RNA was extracted from flower petals using a MiniBEST universal RNA extraction kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. RNA quality and integrity were assessed using formamide denaturing gel electrophoresis, and RNA quantity was measured using spectrophotometry . cDNA was then synthesized using a Superscript preamplification kit (Invitrogen, California, USA) according to the manufacturer’s instructions. .. Forward (ERF61f1) and reverse (ERF61r1) primers were designed and used to amplify the full-length ERF61 cDNA sequence under the following PCR amplification conditions: 94 °C for 4 min followed by 33 cycles of 94 °C for 30 s, 55 °C for 45 s, and 72 °C for 60 s. The PCR product was cloned into the pMD19-T vector (TaKaRa) and sequenced.

    Spectrophotometry:

    Article Title: Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents, β-ionone and linalool
    Article Snippet: Total RNA was extracted from flower petals using a MiniBEST universal RNA extraction kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions. .. RNA quality and integrity were assessed using formamide denaturing gel electrophoresis, and RNA quantity was measured using spectrophotometry . cDNA was then synthesized using a Superscript preamplification kit (Invitrogen, California, USA) according to the manufacturer’s instructions. .. Forward (ERF61f1) and reverse (ERF61r1) primers were designed and used to amplify the full-length ERF61 cDNA sequence under the following PCR amplification conditions: 94 °C for 4 min followed by 33 cycles of 94 °C for 30 s, 55 °C for 45 s, and 72 °C for 60 s. The PCR product was cloned into the pMD19-T vector (TaKaRa) and sequenced.



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