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superscript ii preamplification kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript ii preamplification kit
    Superscript Ii Preamplification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+preamplification+kit/pm32950676-99-5-9
    Average 90 stars, based on 1 article reviews
    superscript ii preamplification kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Palmitic acid aggravates inflammation of pancreatic acinar cells by enhancing unfolded protein response induced CCAAT-enhancer-binding protein β-CCAAT-enhancer-binding protein α activation.
    Article Snippet: Hypertriglyceridemia is an independent risk factor for acute pancreatitis, in which the pathological mechanisms are not fully illustrated.. Intracellular inflammatory response is a key pathological response in acute pancreatitis and endoplasmic reticulum stress has been suggested to induce inflammation and CCAAT-enhancer-binding protein expression.. Therefore, the current study aims to elucidate the possible relationship between endoplasmic reticulum stress and inflammation in hypertriglyceridemia associated pancreatitis and the possible involvement of CCAAT-enhancer-binding protein.

    Article Title: In vitro Culture of Human Testicular Stem Cells on Feeder-Free Condition
    Article Snippet: .. Reverse transcriptase polymerase chain reaction (PCR) was carried out by first-strand cDNA synthesized by random hexamers and the Superscript II preamplification system (Invitrogen). ..

    Article Title: Substance P-regulated leukotriene B4 production promotes acute pancreatitis-associated lung injury through neutrophil reverse migration.
    Article Snippet: Leukotriene B4 (LTB4) is a potent chemoattractant and inflammatory mediator involved in multiple inflammatory diseases.. Substance P (SP) has been reported to promote production of LTB4 in itch-associated response in vivo and in some immune cells in vitro.. Here, we investigated the role of LTB4 in acute pancreatitis (AP), AP-associated acute lung injury (ALI) and the related mechanisms of LTB4 production in AP.

    Article Title: Dopamine D 2 receptor signalling controls inflammation in acute pancreatitis via a PP2A‐dependent Akt/NF‐κB signalling pathway
    Article Snippet: Total RNA was isolated using Trizol reagent (Invitrogen, CA, USA). .. RNA was subjected to reverse transcription using a commercial SuperScript II preamplification kit (Fermentas, MD, USA); the purity of RNA products was determined by 260/280 ratio to be between 1.8 and 2.0. ..

    Article Title: Pituitary Adenylate Cyclase-Activating Peptide in the Bed Nucleus of the Stria Terminalis Mediates Stress-Induced Reinstatement of Cocaine Seeking in Rats
    Article Snippet: Briefly, after euthanasia of rats by rapid decapitation, coronal rat brain sections were prepared using a rodent brain matrix (Ted Pella), and micropunched BNST tissues were frozen for subsequent total RNA extraction using STAT60 RNA/mRNA isolation reagent (Tel-Test ‘B’, Friendswood, TX). .. All RNAs were reverse transcribed using random hexamer primers with SuperScript II Preamplification System (Invitrogen, Carlsbad, CA). ..

    other:

    Article Title: Identification of cell-penetrating osteogenic peptide from copine-7 protein and its delivery system for enhanced bone formation.
    Article Snippet: Peptide and proteins are recognized as highly selective and therapeutically active biomaterials, as well as relatively safe in clinical application.. A calcium phospholipid-binding protein, copine VII (CPNE7), has been recently identified to induce hard tissue regeneration, including bone and dentin by internalizing into the cells.. However, the clinical application of the full length of CPNE7 has limited due to its large size with short half-life.

    Article Title: Cell-penetrating superoxide dismutase attenuates oxidative stress-induced senescence by regulating the p53-p21 Cip1 pathway and restores osteoblastic differentiation in human dental pulp stem cells
    Article Snippet: At the end of the indicated times, total RNA was isolated using TRIzol RNA isolation (Invitrogen; Life Technologies) and quantified by ultraviolet spectroscopy.

    Polymerase Chain Reaction:

    Article Title: In vitro Culture of Human Testicular Stem Cells on Feeder-Free Condition
    Article Snippet: .. Reverse transcriptase polymerase chain reaction (PCR) was carried out by first-strand cDNA synthesized by random hexamers and the Superscript II preamplification system (Invitrogen). ..

    Synthesized:

    Article Title: In vitro Culture of Human Testicular Stem Cells on Feeder-Free Condition
    Article Snippet: .. Reverse transcriptase polymerase chain reaction (PCR) was carried out by first-strand cDNA synthesized by random hexamers and the Superscript II preamplification system (Invitrogen). ..

    Random Hexamer:

    Article Title: Pituitary Adenylate Cyclase-Activating Peptide in the Bed Nucleus of the Stria Terminalis Mediates Stress-Induced Reinstatement of Cocaine Seeking in Rats
    Article Snippet: Briefly, after euthanasia of rats by rapid decapitation, coronal rat brain sections were prepared using a rodent brain matrix (Ted Pella), and micropunched BNST tissues were frozen for subsequent total RNA extraction using STAT60 RNA/mRNA isolation reagent (Tel-Test ‘B’, Friendswood, TX). .. All RNAs were reverse transcribed using random hexamer primers with SuperScript II Preamplification System (Invitrogen, Carlsbad, CA). ..



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