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Lifetech Scientific Corporation superscript iv buffer
Superscript Iv Buffer, supplied by Lifetech Scientific Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iv/iii+reverse+superscript+transcriptase/pm40968292-299-6-9
Average 86 stars, based on 1 article reviews
superscript iv buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Isolation:

Article Title: Fetal and neonatal hematopoietic progenitors are functionally and transcriptionally resistant to Flt3-ITD mutations
Article Snippet: .. RNA was isolated from HSCs with RNAeasy micro plus columns (Qiagen) and converted to cDNA with Superscript III reverse transcriptase (Lifetech, Carlsbad, CA). ..

Reverse Transcription:

Article Title: Fetal and neonatal hematopoietic progenitors are functionally and transcriptionally resistant to Flt3-ITD mutations
Article Snippet: .. RNA was isolated from HSCs with RNAeasy micro plus columns (Qiagen) and converted to cDNA with Superscript III reverse transcriptase (Lifetech, Carlsbad, CA). ..

Article Title: Anti-TL1A antibodies and uses thereof
Article Snippet: RNA was then isolated from PBMC using an RNeasy mini kit with on-column DNase I digestion from Qiagen. .. RNA from each sample was converted to cDNA using the High Capacity cDNA Reverse Transcription Kit according to manufacturer's instructions (LifeTech). .. Allele-specific expression at rs3810936 was determined using TaqMan SNP genotyping assay specific for rs3810936 (LifeTech) and standard protocols on the Bio-Rad droplet digital PCR platform.

Article Title: Helicobacter bilis Infection Accelerates and H. hepaticus Infection Delays the Development of Colitis in Multiple Drug Resistance-Deficient (mdr1a−/−) Mice
Article Snippet: .. cDNA was synthesized from 5 g of DNA-free treated (AMBION, Austin, TX) total RNA using mixture of 125 ng of oligodT and 37.5 ng of random hexamers and Superscript II reverse transcriptase (Lifetech, Gaithersburg, MD) per manufacturer’s instructions. ..

Article Title: The Transmembrane Form of the CX3CL1 Chemokine Fractalkine Is Expressed Predominantly by Epithelial Cells in Vivo
Article Snippet: .. Total RNA was reverse-transcribed using oligo dT12–18 and Superscript reverse transcriptase (Lifetech). ..

Article Title: Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs
Article Snippet: .. Each 50-μl well reaction contained 1× SuperScript IV buffer (Lifetech, 18090050), 500 μM of each dNTP (New England Biolabs, N0447L), 1 μM RT primer (Integrated DNA Technologies), 0.2 mg ml −1 BSA (New England Biolabs, B9200S), 0.8 U per μl RNase inhibitor (M0314L or Thermo Fisher Scientific, EO0384) and 20 U per μl of SuperScript IV reverse transcriptase (Lifetech, 18090050) in RNase-free water. ..

Article Title: Establishment of Human Brain Organoid Models of Tuberous Sclerosis
Article Snippet: RNA levels and purity were assessed with a NanoDrop spectrophotometer. .. Reverse transcription was performed using random hexamer primers and Superscript III reverse transcriptase (Lifetech: 18080051). .. Realtime PCR was performed in triplicate on 600 ng of cDNA using an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System with SYBR FAST qPCR Master Mix (Kapa Biosystems: 07959389001).

Synthesized:

Article Title: Helicobacter bilis Infection Accelerates and H. hepaticus Infection Delays the Development of Colitis in Multiple Drug Resistance-Deficient (mdr1a−/−) Mice
Article Snippet: .. cDNA was synthesized from 5 g of DNA-free treated (AMBION, Austin, TX) total RNA using mixture of 125 ng of oligodT and 37.5 ng of random hexamers and Superscript II reverse transcriptase (Lifetech, Gaithersburg, MD) per manufacturer’s instructions. ..

other:

Article Title: Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs.
Article Snippet: The library was sequenced on an Illumina NovaSeq X Plus 10B (150-bp paired-end reads), targeting ~5 million reads per sample.

Random Hexamer:

Article Title: Establishment of Human Brain Organoid Models of Tuberous Sclerosis
Article Snippet: RNA levels and purity were assessed with a NanoDrop spectrophotometer. .. Reverse transcription was performed using random hexamer primers and Superscript III reverse transcriptase (Lifetech: 18080051). .. Realtime PCR was performed in triplicate on 600 ng of cDNA using an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System with SYBR FAST qPCR Master Mix (Kapa Biosystems: 07959389001).



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